Hydrogel-Based Colorimetric Assay for Multiplexed MicroRNA Detection in a Microfluidic Device [PDF]
, 2020 Although microRNA (miRNA) expression levels provide important information regarding disease states owing to their unique dysregulation patterns in tissues, translation of miRNA diagnostics into point-of-care (POC) settings has been limited by practical ...
Doyle, Patrick S. +3 more
core +1 more source
Biosensors and Bioelectronics: X, 2023 The recent emergence of human coronaviruses (CoVs) causing severe acute respiratory syndrome (SARS) is posing a great threat to global public health. Therefore, the rapid and accurate identification of pathogenic viruses plays a vital role in selecting ...
Xucheng Huang +4 more
doaj +1 more source
Aptamer-based therapeutics and their potential in radiopharmaceutical design [PDF]
, 2007 Aptamers, short, single stranded oligonucleotide entities, have been developed in the past 15 years against a plethora of targets and for a variety of applications.
Ferreira, Catia S. M. +1 more
core +3 more sources
Family-specific degenerate primer design: a tool to design consensus degenerated oligonucleotides [PDF]
, 2013 Designing degenerate PCR primers for templates of unknown nucleotide sequence may be a very difficult task. In this paper, we present a new method to design degenerate primers, implemented in family-specific degenerate primer design (FAS-DPD) computer ...
Borio, Cristina Silvia +5 more
core +3 more sources
A comparison of nucleic acid amplification techniques for the assessment of bacterial viability [PDF]
Letters in Applied Microbiology, 2001 The ability to determine the presence and viability status of bacteria by molecular methods could offer significant advantages to the food, environmental and health sectors, in terms of improved speed and sensitivity of detection.In this study, we have assessed three amplification techniques, PCR, RT-PCR and NASBA, for their ability to detect nucleic ...
L, Birch +3 more
openaire +2 more sources
Novel approaches toward preferential detection of viable cells using nucleic acid amplification techniques [PDF]
FEMS Microbiology Letters, 2009 This article elaborates on possible future directions for microbial viability assessment using nucleic acid-modifying compounds in combination with DNA- (and potentially RNA-) amplification technologies. Bacteria were traditionally considered viable when they could be cultured, whereas today's viability concept is based on the presence of some form of ...
Andreas, Nocker, Anne K, Camper
openaire +2 more sources
Cost analysis of nucleic acid amplification for diagnosing pulmonary tuberculosis, within the context of the Brazilian Unified Health Care System [PDF]
Jornal Brasileiro de Pneumologia, 2015 We estimated the costs of a molecular test for Mycobacterium tuberculosis and resistance to rifampin (Xpert MTB/RIF) and of smear microscopy, within the Brazilian Sistema Único de Saúde (SUS, Unified Health Care System). In SUS laboratories in the cities
Márcia Pinto +3 more
doaj +3 more sources
Nanomaterials for Healthcare Biosensing Applications [PDF]
, 2019 In recent years, an increasing number of nanomaterials have been explored for their applications in biomedical diagnostics, making their applications in healthcare biosensing a rapidly evolving field.
Altintas, Zeynep, Pirzada, Muqsit
core +1 more source
The newfound relationship between extrachromosomal DNAs and excised signal circles
FEBS Letters, EarlyView.Extrachromosomal DNAs (ecDNAs) contribute to the progression of many human cancers. In addition, circular DNA by‐products of V(D)J recombination, excised signal circles (ESCs), have roles in cancer progression but have largely been overlooked. In this Review, we explore the roles of ecDNAs and ESCs in cancer development, and highlight why these ...
Dylan Casey, Zeqian Gao, Joan Boyes
wiley +1 more source
Nucleic Acid-Based Sensing Techniques for Diagnostics and Surveillance of Influenza
Biosensors, 2021 Influenza virus poses a threat to global health by causing seasonal outbreaks as well as three pandemics in the 20th century. In humans, disease is primarily caused by influenza A and B viruses, while influenza C virus causes mild disease mostly in ...
Samantha J. Courtney +2 more
doaj +1 more source