Results 1 to 10 of about 205,585 (348)
Nucleic acid stain-dependent single strand conformation polymorphisms [PDF]
BioTechniques, 2004 Single strand conformational poly-morphism (SSCP) is a mobility shift analysis of single-stranded DNA (ss-DNA) by nondenaturing polyacryl-amide gel electrophoresis to detect DNA sequence polymorphisms (1). Typical SSCP protocols detect single strands by autoradiography (2–4) or by treatment with a DNA stain after electrophoresis (5–12).
David R. McKee, M. Scott Thomson
doaj +4 more sources
The conformation of single‐stranded nucleic acids [PDF]
European Journal of Biochemistry, 1990 Conformational analyses using the single‐strand‐specific nuclease from mung bean and restriction endonucleases have been performed on a series of DNA fragments related to the sequence of the yeast initiator tRNAMet. Mung bean nuclease cleaves DNA fragments exclusively in some, but not all, single‐stranded regions as predicted by RNA secondary rules ...
Jean Paquette +4 more
openalex +4 more sources
Conformational Diversity Versus Nucleic Acid Triplex Stability, a Combinatorial Study [PDF]
Journal of Biological Chemistry, 2001 The stability of a triple helix formed between a DNA duplex and an incoming oligonucleotide strand strongly depends on the solvent conditions and on intrinsic chemical and conformational factors. Attempts to increase triple helix stability in the past included chemical modification of the backbone, sugar ring, and bases in the third strand.
Eloy Bernal‐Méndez +1 more
openalex +3 more sources
Electrostatics of Nucleic Acid Folding under Conformational Constraint [PDF]
Journal of the American Chemical Society, 2012 RNA folding is enabled by interactions between the nucleic acid and its ion atmosphere, the mobile sheath of aqueous ions that surrounds and stabilizes it. Understanding the ion atmosphere requires the interplay of experiment and theory. However, even an apparently simple experiment to probe the ion atmosphere, measuring the dependence of DNA duplex ...
Peter C. Anthony +5 more
openalex +4 more sources
Nucleic Acids: Conformation Contorted [PDF]
Nature, 1973 Our Molecular Biology Correspondent
openalex +2 more sources
Nucleic Acid Reactivity and Conformation
Journal of Biological Chemistry, 1973 Robert Shapiro +3 more
openalex +2 more sources
Conformations of Single-Stranded Nucleic Acids in Solution [PDF]
Biophysical Journal, 2017 Alex Plumridge +2 more
openalex +2 more sources
Modelling aptamers with nucleic acid mimics (NAM): From sequence to three-dimensional docking
PLoS ONE, 2022 Aptamers are single-stranded oligonucleotides, formerly evolved by Systematic Evolution of Ligands by EXponential enrichment (SELEX), that fold into functional three-dimensional structures.
Ricardo Oliveira +5 more
doaj +2 more sources
Developing Community Resources for Nucleic Acid Structures
Life, 2022 In this review, we describe the creation of the Nucleic Acid Database (NDB) at Rutgers University and how it became a testbed for the current infrastructure of the RCSB Protein Data Bank. We describe some of the special features of the NDB and how it has
Helen M. Berman +2 more
doaj +1 more source
Solvent interactions stabilising nucleic acid conformers [PDF]
Nucleic Acids Research, 1989 The transition of oligonucleotides from the B to the A conformation has been studied by the use of simple geometric calculations aimed at finding possible hydration sites which could stabilize these conformations. The method involves the classification of equally spaced grid points, surrounding the oligonucleotide, into groups depending on whether a ...
R J, Elliott, J M, Goodfellow
openaire +2 more sources