Results 41 to 50 of about 451,143 (385)

Microgels and apparatus for PAGE of nucleic acids in one or two dimensions

BioTechniques, 2018
We describe a system for horizontal 1D or 2D PAGE comprising an apparatus and microgels. There is no buffer outside the gel, making handling and sample loading easy.
Hans G Thormar, Gudmundur H Gunnarsson, Bjarki Gudmundsson, Kristjan Leosson, Peter Estibeiro, Jon J Jonsson   +5 more
doaj   +1 more source

Molecular Rotors: Fluorescent Sensors for Microviscosity and Conformation of Biomolecules

Angewandte Chemie, 2023
The viscosity and crowding of biological environment are considered vital for the correct cellular function, and alterations in these parameters are known to underly a number of pathologies including diabetes, malaria, cancer and neurodegenerative ...
Miguel Paez-Perez, M. Kuimova
semanticscholar   +1 more source

IFITs: Emerging Roles As Key Anti-Viral Proteins

Frontiers in Immunology, 2014
Interferon-induced protein with tetratricopeptide repeats (IFITs) are a family of proteins which are strongly induced downstream of type I interferon (IFN) signaling.
Gregory I Vladimer, Maria W Górna, Giulio eSuperti-Furga   +2 more
doaj   +1 more source

First High-Resolution Crystal Structures of DNA:2′-O-Methyl-RNA Heteroduplexes

Crystals, 2022
Heteroduplexes composed of all-DNA and all-2′-OMe RNA strands do not occur in nature, but they have found application in the development of molecular beacons and could also be used as aptamers or elements of nucleic acid-based nanostructures that will ...
Rafał Dolot, Anna Maciaszek, Barbara Mikołajczyk, Barbara Nawrot   +3 more
doaj   +1 more source

RosettaBackrub--a web server for flexible backbone protein structure modeling and design. [PDF]

, 2010
The RosettaBackrub server (http://kortemmelab.ucsf.edu/backrub) implements the Backrub method, derived from observations of alternative conformations in high-resolution protein crystal structures, for flexible backbone protein modeling.
Friedland, Gregory F, Humphris, Elisabeth L, Kortemme, Tanja, Lauck, Florian, Smith, Colin A   +4 more
core   +2 more sources

Rigid spin-labeled nucleoside Ç: a nonperturbing EPR probe of nucleic acid conformation

Nucleic Acids Research, 2008
Rigid spin-labeled nucleoside Ç, an analog of deoxycytidine that base-pairs with deoxyguanosine, was incorporated into DNA oligomers by chemical synthesis.
P. Čekan, Alyssa L. Smith, N. Barhate, B. Robinson, S. Sigurdsson   +4 more
semanticscholar   +1 more source

TFE and Spt4/5 open and close the RNA polymerase clamp during the transcription cycle [PDF]

, 2016
Transcription is an intrinsically dynamic process and requires the coordinated interplay of RNA polymerases (RNAPs) with nucleic acids and transcription factors.
Gietl, Andreas, Grohmann, Dina, Schulz, Sarah, Smollett, Katherine, Tinnefeld, Philip, Werner, Finn   +5 more
core   +1 more source

Use of Nucleic Acid Analogs for the Study of Nucleic Acid Interactions

Journal of Nucleic Acids, 2011
Unnatural nucleosides have been explored to expand the properties and the applications of oligonucleotides. This paper briefly summarizes nucleic acid analogs in which the base is modified or replaced by an unnatural stacking group for the study of ...
Shu-ichi Nakano, Masayuki Fujii, Naoki Sugimoto   +2 more
doaj   +1 more source

Lone Pair…π Contacts and Structure Signatures of r(UNCG) Tetraloops, Z-Turns, and Z-Steps: A WebFR3D Survey

Molecules, 2022
Z-DNA and Z-RNA have long appeared as oddities to nucleic acid scientists. However, their Z-step constituents are recurrently observed in all types of nucleic acid systems including ribosomes.
Craig L. Zirbel, Pascal Auffinger
doaj   +1 more source

Conformational Dynamics of Biopolymers in the Course of Their Interaction: Multifaceted Approaches to the Analysis by the Stopped-Flow Technique with Fluorescence Detection

Photonics, 2023
This review deals with modern approaches to systematic research on molecular-kinetic mechanisms of damage recognition and removal by pro- and eukaryotic enzymes of DNA base excision repair.
Nikita A. Kuznetsov
doaj   +1 more source