Results 91 to 100 of about 323,426 (336)

Rapid generation of chromosome-specific alphoid DNA probes using the polymerase chain reaction [PDF]

, 1992
Non-isotopic in situ hybridization of chromosome-specific alphoid DNA probes has become a potent tool in the study of numerical aberrations of specific human chromosomes at all stages of the cell cycle. In this paper, we describe approaches for the rapid
A Gnirke, AHN Hopman, AR Mitchell, C Huxley, C Lengauer, Christoph Lengauer, D Pinkel, ED Green, EW Jabs, HF Willard, HF Willard, HF Willard, HM Rosenberg, Ian Dunham, J Anastasi, J Meyne, JE Koch, JS Waye, JS Waye, JS Waye, L Manuelidis, L Manuelidis, M Klever, P Emmerich, PJ Poddighe, PR Langer, S Popp, T Cremer, T Cremer, T Cremer, T Imai, T Maniatis, Terry Featherstone, Thomas Cremer   +33 more
core   +1 more source

DNA‑Directed Assembly of Photonic Nanomaterials for Diagnostic and Therapeutic Applications

Advanced Materials, EarlyView.
DNA‐directed assembly offers a powerful strategy for constructing structured photonic nanomaterials with precise spatial control. This review provides a comprehensive overview of recent advancements in DNA‐assembled photonic nanomaterials for diagnostics and therapeutics, highlighting key design principles, functionalization strategies, and optical ...
Longjiang Ding, Bing Liu, Andreas Peil, Sisi Fan, Jie Chao, Na Liu   +5 more
wiley   +1 more source

Functionalized graphene-oxide grids enable high-resolution cryo-EM structures of the SNF2h-nucleosome complex without crosslinking

Nature Communications
Single-particle cryo-EM is widely used to determine enzyme-nucleosome complex structures. However, cryo-EM sample preparation remains challenging and inconsistent due to complex denaturation at the air-water interface (AWI).
Un Seng Chio, Eugene Palovcak, Anton A. A. Smith, Henriette Autzen, Elise N. Muñoz, Zanlin Yu, Feng Wang, David A. Agard, Jean-Paul Armache, Geeta J. Narlikar, Yifan Cheng   +10 more
doaj   +1 more source

Mechanochemistry of nucleosides, nucleotides and related materials

Beilstein Journal of Organic Chemistry, 2018
The application of mechanical force to induce the formation and cleavage of covalent bonds is a rapidly developing field within organic chemistry which has particular value in reducing or eliminating solvent usage, enhancing reaction rates and also in ...
Olga Eguaogie, Joseph S. Vyle, Patrick F. Conlon, Manuela A. Gîlea, Yipei Liang   +4 more
doaj   +1 more source

Detection of genetic variation using dual-labeled peptide nucleic acid (PNA) probe-based melting point analysis

Biological Procedures Online, 2015
Thermal denaturation of probe-target hybrid is highly reproducible, and which makes probe melting point analysis reliable in the detection of mutations, polymorphisms and epigenetic differences in DNA.
Deokhwe Hur, M. Kim, Minsik Song, Jinwook Jung, Heekyung Park   +4 more
semanticscholar   +1 more source

Stimulation of Proteinase K Action by Denaturing Agents: Application to the Isolation of Nucleic Acids and the Degradation of ‘Masked’ Proteins [PDF]

European Journal of Biochemistry, 1975
Hydrolysis of serum albumin by proteinase K was strongly (> 7‐fold) stimulated by urea and dodecylsulfate in a dose‐dependent manner. With an oligopeptide as substrate, however, proteinase K was inactivated by dodecylsulfate. This indicates that the apparent activation of proteinase K by urea and dodecylsulfate is caused primarily by denaturation of
Peter Adamietz, Helmuth Hilz, U. Wiegers
openaire   +3 more sources

Harnessing Photo‐Energy Conversion in Nanomaterials for Precision Theranostics

Advanced Materials, EarlyView.
Harnessing photo‐energy conversion in nanomaterials enables precision theranostics through light‐driven mechanisms such as photoluminescence, photothermal, photoelectric, photoacoustic, photo‐triggered surface‐enhanced Raman scattering (SERS), and photodynamic processes. This review explores six fundamental principles of photo‐energy conversion, recent
Jingyu Shi, Yadi Fan, Qin Zhang, Yingying Huang, Mo Yang   +4 more
wiley   +1 more source

Centrifugal Microfluidic System for Nucleic Acid Amplification and Detection

Italian National Conference on Sensors, 2015
We report here the development of a rapid PCR microfluidic system comprising a double-shaft turntable and centrifugal-based disc that rapidly drives the PCR mixture between chambers set at different temperatures, and the bidirectional flow improved the ...
Baogang Miao, Niancai Peng, Lei Li, Zheng Li, F. Hu, Zengming Zhang, Chaohui Wang   +6 more
semanticscholar   +1 more source

Designing the Next Generation of Biomaterials through Nanoengineering

Advanced Materials, EarlyView.
Nanoengineering enables precise control over biomaterial interactions with living systems by tuning surface energy, defects, porosity, and crystallinity. This review highlights how these nanoscale design parameters drive advances in regenerative medicine, drug delivery, bioprinting, biosensing, and bioimaging, while outlining key translational ...
Ryan Davis Jr., Ishaan Duggal, Nicholas A. Peppas, Akhilesh K. Gaharwar   +3 more
wiley   +1 more source

Biomolecular Condensates as Emerging Biomaterials: Functional Mechanisms and Advances in Computational and Experimental Approaches

Advanced Materials, EarlyView.
Integration of computational and experimental approaches for deciphering the underlying mechanism of biomolecular condensates and potential biomaterials design (Created in Biorender. Raza, Z. (2025) https://BioRender.com/irgmeqi). Abstract Biomolecular condensates, a ubiquitous class of biomaterials found in living cells, have been shown to be ...
Qiang Zhu, Zahra Raza, Dzung Do‐Ha, Emma De Costa, Pavlina Sasheva, Luke McAlary, Hadi Mahmodi, Warwick P. Bowen, Lezanne Ooi, Irina Kabakova, Haibo Yu   +10 more
wiley   +1 more source