Results 141 to 150 of about 252,695 (308)

A Visual and Rapid PCR Test Strip Method for the Authentication of Sika Deer Meat (<i>Cervus nippon</i>). [PDF]

Int J Mol Sci
Gao L, Xie Y, Zhang Y, Yang Y, Yuan G, Xia W.   +5 more
europepmc   +1 more source

Characterisation of a cold‐adapted, thermostable glucokinase from psychrophilic Pseudoalteromonas sp. AS‐131 reveals how the enzyme achieves high thermal stability without loss of cold adaptation

The FEBS Journal, EarlyView.
We investigated glucokinase from the psychrophilic Pseudoalteromonas sp. AS‐131 (PsGK), which was isolated from the Antarctic Ocean. Biochemical studies revealed that PsGK is a cold‐adapted enzyme with high thermal stability, in contrast to general cold‐adapted enzymes, which have low thermal stability.
Akane Yato, Yuki Kato, Fuyuko Hayashi, Rio Asaka, Atsuko Ogawa, Tokuro Oda, Sayaka Tsuji, Masashi Unno, Nobuaki Soh, Keiichi Watanabe, Masaki Horitani   +10 more
wiley   +1 more source

A Hanks‐type bacterial kinase, PknS, directly phosphorylates the alternative sigma factor EcfK to promote resistance to protist predation

The FEBS Journal, EarlyView.
The Xanthomonas citri Hanks‐type kinase PknS autophosphorylates and directly phosphorylates the alternative sigma factor EcfK at five residues. Besides the conserved residue T51 in the σ2 domain, phosphorylation of a residue in the linker between σ2 and σ4 is critical for EcfK activation by promoting its interaction with a positively charged pocket in ...
Lídia dos Passos Lima, Dev Sriranganadane, Daiane Laise da Silva, Natália C. Drebes Dörr, Enzo Breviglieri Sichi Mello, Caio Vinicius dos Reis, Rogério Ferreira Lourenço, José Felipe Teixeira da Silva Santos, Anita Salmazo, Brenno Wendler Miranda, Katlin B. Massirer, Rafael M. Couñago, Cristina E. Alvarez‐Martinez   +12 more
wiley   +1 more source

Making Molecular Diagnostics Faster

International Journal of Laboratory Hematology, EarlyView.
ABSTRACT Background Over the past 40 years, molecular diagnostic methods have evolved from multi‐step, time‐consuming protocols towards either rapid targeted tests or expansive, massively parallel testing. Aims Here we consider the speed limits of targeted molecular diagnostics, considering the three sequential required steps: nucleic acid preparation,
Carl T. Wittwer, Luming Zhou, Felix Ye, Adam Millington, Adrian de Cola, Noriko Kusukawa   +5 more
wiley   +1 more source

First Description and Characterisation of Lactococcus garvieae Strains Causing Septicaemic Disease in Farmed Sea Bass (Dicentrarchus labrax; Linnaeus) in Spain

Journal of Fish Diseases, EarlyView.
ABSTRACT This study presents the first description and characterisation of Lactococcus garvieae isolated from diseased European sea bass (Dicentrarchus labrax) farmed in Spain. During autumn 2023, two fish farms were affected by infectious outbreaks causing moderate cumulative fish mortality (5%–10%). Diseased fish showed clinical signs of haemorrhagic
Belén Fouz, Evelyn Carballeda‐Carrasco, Javier Barriga‐Cuartero, Yolanda Torres‐Corral, Álvaro Robles, Carlos Zarza, Ysabel Santos   +6 more
wiley   +1 more source

Development and clinical application of a rapid qPCR instrument featuring three independent temperature modules and a time-based algorithm for respiratory pathogen diagnosis. [PDF]

Virol J
Wang Y, Wang H, Zhou M, Wang M, Wu T, Zhou S, Liao X, Liu W, Zhuang X, Zhou Z, Zhou R.   +10 more
europepmc   +1 more source

A single‐MYB transcription factor GmMYB331 regulates seed oil accumulation and seed size/weight in soybean

Journal of Integrative Plant Biology, EarlyView.
The soybean transcription factor GmMYB331 regulates oil content and seed size. Moderate expression of GmMYB331 significantly enhanced seed oil accumulation, and strong expression promoted increased seed size. This research provides a genetic resource and theoretical foundation for high‐yield and quality breeding in soybean.
Zhou‐Ya Wang, Lu‐Yao Zhang, Bin Zhou, Jing‐Jing Liang, Yan‐Bao Tian, Zhi‐Hao Jiang, Jian‐Jun Tao, Cui‐Cui Yin, Shou‐Yi Chen, Wan‐Ke Zhang, Jin‐Song Zhang, Wei Wei   +11 more
wiley   +1 more source

Bridging PCR-Based Genome-Walking Protocol. [PDF]

Bio Protoc
Li M, Gu Y, Tang Q, Li H.
europepmc   +1 more source

Imaging malaria parasites across scales and time

Journal of Microscopy, EarlyView.
Abstract The idea that disease is caused at the cellular level is so fundamental to us that we might forget the critical role microscopy played in generating and developing this insight. Visually identifying diseased or infected cells lays the foundation for any effort to curb human pathology.
Julien Guizetti
wiley   +1 more source

DNA Persistent Length in Solutions of Different pH. [PDF]

Int J Mol Sci
Kasyanenko N, Khansetsen B, Baryshev A, Sokolov P.   +3 more
europepmc   +1 more source