Results 141 to 150 of about 323,426 (336)

Hybridization Probes Featuring a Pyrenylpyridine C‐Nucleoside or Its Palladacycle as a Fluorescent Sensor Moiety

ChemBioChem, EarlyView.
When placed in the middle of a double helix as a nucleobase surrogate, pyren‐1‐ylpyridine and its palladacyclic derivative show fluorescence emission sensitive to the identity of the canonical nucleobase opposite to it. Emission of the palladacycle is weaker but the base discrimination stronger, possibly because of Pd(II)‐mediated base pairing.
Dattatraya Uttam Ukale, Tuomas Lönnberg
wiley   +1 more source

Milligram‐Scale Enzymatic Synthesis of Base‐Modified DNA through Primer Extension: Polymerase Synthesis and NMR Structures of DNA Containing Phenyl‐Substituted Nucleobases

ChemistryEurope, EarlyView.
Larger‐scale enzymatic synthesis of base‐modified DNA is developed using primer extension with KOD(exo‐) DNA polymerase allowing preparation of milligram amounts of the modified DNA sufficient for NMR structural analysis. Milligram (≈200 nmol) scale enzymatic synthesis of base‐modified DNA is developed based on primer extension using easily accessible ...
Veronika Raindlová, Klára Viktorinová, Michal Tichý, Lucie Bednárová, Pavel Srb, Miguel Riopedre‐Fernandez, Michal Hocek   +6 more
wiley   +1 more source

Thermal denaturation of double-stranded nucleic acids: prediction of temperatures critical for gradient gel electrophoresis and polymerase chain reaction [PDF]

, 1994
Gerhard Steger
openalex   +1 more source

Safety, Tolerability, and Pharmacokinetics of the Long‐Acting SARS‐CoV‐2–Neutralizing Monoclonal Antibody Combination AZD7442 (Tixagevimab/Cilgavimab) in Healthy Chinese Adults

Clinical Pharmacology in Drug Development, EarlyView.
Abstract AZD7442, a combination of extended half‐life monoclonal antibodies tixagevimab and cilgavimab, was shown to neutralize previously circulating SARS‐CoV‐2 variants. This study evaluated safety, tolerability, pharmacokinetics, and pharmacodynamics of AZD7442 in healthy Chinese adults. In this randomized, placebo‐controlled, Phase 1 study, AZD7442
Nanyang Li, Jing Zhang, Wenhong Zhang, Zhongyuan Xu, Xiangcao Yao, Anqi He, Shuyuan Liu, Xiaoyun Ge, Jinxi Liu, Yunfei Li, Cecil Chi‐Keung Chen, Huixia Zhang   +11 more
wiley   +1 more source

Intestinal spirochaetes of the genus Brachyspira share a partially conserved 26 kilobase genomic region with Enterococcus faecalis and Escherichia coli [PDF]

, 2008
Anaerobic intestinal spirochaetes of the genus Brachyspira include both pathogenic and commensal species. The two best-studied members are the pathogenic species B. hyodysenteriae (the aetiological agent of swine dysentery) and B.
Bellgard, M.I., Dunn, D.S., Hampson, D.J., La, T., Motro, Y., Phillips, N.D.   +5 more
core   +3 more sources

From One Strand Dyed/Undyed Hair With/Without Root to Fast and Successful STR Profiling and Evaluation With Principle Component Analysis

ELECTROPHORESIS, EarlyView.
ABSTRACT Fallen/shed single hair shafts with/without roots can be encountered as evidence in crime scenes. Identification from hair exhibits serious difficulties, due to the low amount of DNA, PCR inhibitors, and the existence of DNA mostly in degraded forms. Exposure of hair to chemical oxidation, sunlight, etc.
Gulten Rayimoglu, Fatma Cavus Yonar, Beril Anılanmert   +2 more
wiley   +1 more source

Allelic Frequency of Kappa-Casein Locus (Asp148/Ala) in F1: Simmental (Bos Taurus) X Ongole Grade (Bos Indicus) [PDF]

, 2012
This study was conducted to detect the genetic variants (single nucleotide polymorphism) of kappa-casein locus (Asp148/Ala) in F1: Simmental (Bos taurus) x Ongole grade (Bos indicus), SIMPO.
Mu\u27in, M. A. (M), Supriyantono, A. (A)   +1 more
core   +1 more source

Critical Evaluation of Methods for the Identification of Aneugens

Environmental and Molecular Mutagenesis, EarlyView.
ABSTRACT The genotoxic potential of chemicals must be evaluated in regulatory safety assessment settings, including but not limited to, the development of new pharmaceuticals, industrial chemicals, food and cosmetic ingredients, and agrochemicals. Initial assessment of the chromosome‐damaging potential of chemicals is often conducted in mammalian cells
Xiaowen Sun, Stephen D. Dertinger, Azeddine Elhajouji, Daniel J. Roberts, Jan van Benthem, Maik Schuler, Shambhu Roy, Alexandra Taraboletti, Connie L. Chen   +8 more
wiley   +1 more source

Recent advances in isothermal amplification techniques coupled with clustered regularly interspaced short palindromic repeat/Cas systems

Interdisciplinary Medicine, EarlyView.
Abstract Isothermal amplification (IA) techniques have emerged as promising alternatives to polymerase chain reaction (PCR), enabling rapid and efficient nucleic acid amplification at constant temperatures. Meanwhile, clustered regularly interspaced short palindromic repeat (CRISPR)/Cas‐based technology has revolutionized molecular diagnostics by ...
Miao Liu, Yajun Hou, Yuhang Cheng, Ziyun Li, Jiawei Zeng, Li Li, Jinyong Luo, Bo Shen   +7 more
wiley   +1 more source

Solid Phase Synthesis and TAR RNA-Binding Activity of Nucleopeptides Containing Nucleobases Linked to the Side Chains via 1,4-Linked-1,2,3-triazole

Biomedicines
Nucleopeptides (NPs) represent synthetic polymers created by attaching nucleobases to the side chains of amino acid residues within peptides. These compounds amalgamate the characteristics of peptides and nucleic acids, showcasing a unique ability to ...
Piotr Mucha, Małgorzata Pieszko, Irena Bylińska, Wiesław Wiczk, Jarosław Ruczyński, Katarzyna Prochera, Piotr Rekowski   +6 more
doaj   +1 more source