Unlocked nucleic acid (UNA) and UNA derivatives: Thermal denaturation studies [PDF]
Nucleic Acids Symposium Series, 2008 A study on the thermal stability of duplexes formed between unlocked nucleic acid (UNA) modified DNA and RNA oligonucleotides and complementary DNA and RNA is presented. The acyclic UNA monomers are shown to induce a decrease in duplex thermal stability.
Troels B. Jensen +2 more
semanticscholar +6 more sources
Computer programs for analysis of nucleic acid hybridization, thermal denaturation, and gel electrophoresis data [PDF]
Nucleic Acids Research, 1979 Computer programs for the analysis of data from techniques frequently used in nucleic acids research are described. In addition to calculating non-linear, least-squares solutions to equations describing these systems, the programs allow for data editing, normalization, plotting and storage, and are flexible and simple to use.
Robert F. Murphy +2 more
semanticscholar +5 more sources
Cytofluorometric studies on conformation of nucleic acids in situ. II. Denaturation of deoxyribonucleic acid. [PDF]
Journal of Histochemistry & Cytochemistry, 1976 Thermal denaturation of deoxyribonucleic acid (DNA) in situ in individual unbroken cells is studied by a cytofluorometric method. This method allows us to investigate DNA denaturation in the presence of divalent cations at concentrations reported to be necessary to maintain native structure of nuclear chromatin.
Zbigniew Darżynkiewicz +4 more
semanticscholar +4 more sources
A new approach for detecting HPV DNA in cervical swabs: comparison of nucleic acid extraction with direct PCR [PDF]
Virology JournalBackground Almost all cases of cervical cancer are associated with persistent high-risk HPV infection. WHO prioritizes primary HPV testing for cervical cancer screening.
Fatih Çubuk +6 more
doaj +3 more sources
Applied Sciences, 2022 Peptide nucleic acid (PNA) is an analog of natural nucleic acids, where the sugar-phosphate backbone of DNA is replaced by an electrostatically neutral N-(2-aminoethyl)glycine backbone.
Yuichiro Aiba +2 more
doaj +2 more sources
An improved nucleic acid sequence-based amplification method mediated by T4 gene 32 protein.
PLoS ONE, 2022 The uptake of Nucleic Acid Sequence-Based Amplification (NASBA) for point of care testing may be hindered by a complexity in the workflow due the requirement of a thermal denaturation step to initiate the cyclic isothermal amplification before the ...
Yi Heng Nai +2 more
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Denaturation of proteins and nucleic acids by thermal-gradient electrophoresis [PDF]
Biochemical Journal, 1981 A polyacrylamide-gel-electrophoresis method has been developed that permits the analysis of conformational changes that occur during the thermal denaturation of macromolecules. A stable transverse temperature gradient was produced in an aluminium heating jacket clamped around a vertical polyacrylamide slab gel.
D R Thatcher, B A Hodson
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Synthesis and Evaluation of Artificial Nucleic Acid Bearing an Oxanorbornane Scaffold
Molecules, 2020 Natural oligonucleotides have many rotatable single bonds, and thus their structures are inherently flexible. Structural flexibility leads to an entropic loss when unwound oligonucleotides form a duplex with single-stranded DNA or RNA.
Hibiki Komine +8 more
doaj +2 more sources
Forster resonance energy transfer for studying nucleic acids denaturation: A chemical and biological sciences laboratory experiment [PDF]
Biochemistry and Molecular Biology Education, 2020 AbstractThe Förster resonance energy transfer (FRET) melting assay intends to evaluate the unfolding, denaturation process of DNA secondary structures, and its stabilization using compounds known as DNA binders, some of which are highly specific for G‐quadruplex DNAs versus duplex DNAs.
Josué Carvalho, Carla Cruz
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Nucleic acid denaturation at an electrode surface [PDF]
, 2011 This thesis describes the development and improvement of an assay for the detection and discrimination of DNA sequences based on surface enhanced Raman spectroscopy (SERS) and electrochemically driven denaturation. A DNA helix can be immobilised at a nano-structured gold electrode and then denatured into its constituent components by driving the ...
Robert P. Johnson
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