Forster resonance energy transfer for studying nucleic acids denaturation: A chemical and biological sciences laboratory experiment [PDF]
Biochemistry and Molecular Biology Education, 2020 AbstractThe Förster resonance energy transfer (FRET) melting assay intends to evaluate the unfolding, denaturation process of DNA secondary structures, and its stabilization using compounds known as DNA binders, some of which are highly specific for G‐quadruplex DNAs versus duplex DNAs.
Josué Carvalho, Carla Cruz
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Nucleic acid binding and other biomedical properties of artificial oligolysines
International Journal of Nanomedicine, 2016 Giovanni N Roviello,1 Caterina Vicidomini,1 Vincenzo Costanzo,1 Valentina Roviello2 1CNR Istituto di Biostrutture e Bioimmagini, Via Mezzocannone site and Headquarters, 2Centro Regionale di Competenza (CRdC) Tecnologie, Via Nuova Agnano, Napoli, Italy ...
Roviello GN +3 more
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Partial Denaturation of Double-Stranded DNA on Pristine Graphene under Physiological-like Conditions
Liquids, 2023 Interactions between DNA and graphene are paramount for a wide range of applications, such as biosensing and nanoelectronics; nonetheless, the molecular details of such interactions remain largely unexplored.
Fernando J. A. L. Cruz, José P. B. Mota
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Electrostatic surface plasmon resonance: Direct electric field-induced hybridization and denaturation in monolayer nucleic acid films and label-free discrimination of base mismatches [PDF]
Proceedings of the National Academy of Sciences of the United States of America, 2001 R. J. Heaton +2 more
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Journal of Veterinary Diagnostic Investigation, 2013 M. E. Schroeder +5 more
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Nucleic acid denaturation at an electrode surface
, 2011 This thesis describes the development and improvement of an assay for the detection and discrimination of DNA sequences based on surface enhanced Raman spectroscopy (SERS) and electrochemically driven denaturation. A DNA helix can be immobilised at a nano-structured gold electrode and then denatured into its constituent components by driving the ...
Robert P. Johnson
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Fast and non-toxic in situ hybridization without blocking of repetitive sequences. [PDF]
PLoS ONE, 2012 Formamide is the preferred solvent to lower the melting point and annealing temperature of nucleic acid strands in in situ hybridization (ISH). A key benefit of formamide is better preservation of morphology due to a lower incubation temperature. However,
Steen H Matthiesen, Charles M Hansen
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Ultrafast Microfluidic PCR Thermocycler for Nucleic Acid Amplification
Micromachines, 2023 The polymerase chain reaction (PCR) is essential in nucleic acid amplification tests and is widely used in many applications such as infectious disease detection, tumor screening, and food safety testing; however, most PCR devices have inefficient ...
Yi-Quan An +10 more
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Single-tube collection and nucleic acid analysis of clinical samples for SARS-CoV-2 saliva testing
Scientific Reports, 2022 The SARS-CoV-2 pandemic has brought to light the need for expedient diagnostic testing. Cost and availability of large-scale testing capacity has led to a lag in turnaround time and hindered contact tracing efforts, resulting in a further spread of SARS ...
K. Cole +5 more
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Journal of Advanced Veterinary and Animal Research, 2020 Objective: The study of an in vitro embryosis is crucial in genetics for breed improvement and reproduction in livestock, identifying the causes of infertility, and stem cell application.
Dae Seung Kim +2 more
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