Results 31 to 40 of about 252,695 (308)

Individual Case Analysis of Postmortem Interval Time on Brain Tissue Preservation. [PDF]

open access: yesPLoS ONE, 2016
At autopsy, the time that has elapsed since the time of death is routinely documented and noted as the postmortem interval (PMI). The PMI of human tissue samples is a parameter often reported in research studies and comparable PMI is preferred when ...
Jeffrey A Blair   +11 more
doaj   +1 more source

Single-cell analysis reveals microbial spore responses to microwave radiation

open access: yesJournal of Innovative Optical Health Sciences, 2023
To determine the effects of microwave radiation at the molecular level as well as on the germination, growth and morphology of dry spores at the single-cell level.
Siyi Qiu, Haihua Fan, Lin He
doaj   +1 more source

Physiological and biochemical responses of Yarrowia lipolytica to dehydration induced by air-drying and freezing. [PDF]

open access: yesPLoS ONE, 2014
Organisms that can withstand anhydrobiosis possess the unique ability to temporarily and reversibly suspend their metabolism for the periods when they live in a dehydrated state.
Caroline Pénicaud   +6 more
doaj   +1 more source

Fabrication of Silver Nanoparticles Against Fungal Pathogens

open access: yesFrontiers in Nanotechnology, 2021
The use of silver nanoparticles (AgNPs) against various pathogens is now being well recognized in the agriculture and health sector. Nanoparticles have been shown to exhibit various novel properties and these properties, on other hand, rely upon the size,
Sheikh Mansoor   +6 more
doaj   +1 more source

Atomic scissors: a new method of tracking the 5-bromo-2'-deoxyuridine-labeled DNA in situ. [PDF]

open access: yesPLoS ONE, 2012
A new method of the light microscopy detection of BrdU-labeled DNA in situ is described. It is based on the oxidative attack at the deoxyribose moiety by copper(I) in the presence of oxygen, which leads to the abstraction of hydrogen atom from ...
Anna Ligasová   +4 more
doaj   +1 more source

Polymerase chain reaction in ocular and adnexal inflammation: Our experience with review of literature

open access: yesTNOA Journal of Ophthalmic Science and Research, 2021
Polymerase chain reaction (PCR) is a revolutionary innovation involving enzymatic amplification of nucleic acid sequences in repeated cycles of denaturation, oligonucleotide annealing, and DNA polymerase extension.
Arkaprava Pradhan   +2 more
doaj   +1 more source

Microgels and apparatus for PAGE of nucleic acids in one or two dimensions

open access: yesBioTechniques, 2018
We describe a system for horizontal 1D or 2D PAGE comprising an apparatus and microgels. There is no buffer outside the gel, making handling and sample loading easy.
Hans G Thormar   +5 more
doaj   +1 more source

Probing structural heterogeneities and fluctuations of nucleic acids and denatured proteins [PDF]

open access: yesProceedings of the National Academy of Sciences, 2005
We study protein and nucleic acid structure and dynamics using single-molecule FRET and alternating-laser excitation. Freely diffusing molecules are sorted into subpopulations based on single-molecule signals detected within 100 μs to 1 ms.
Laurence, T A   +3 more
openaire   +3 more sources

C5-Azobenzene-substituted 2'-Deoxyuridine-containing Oligodeoxynucleotides for Photo-Switching Hybridization

open access: yesMolecules, 2014
A new photoisomeric nucleoside dUAz bearing an azobenzene group at the C5-position of 2'-deoxyuridine was designed and synthesized. Photoisomerization of dUAz in oligodeoxynucleotides can be achieved rapidly and selectively with 365 nm (forward) and 450
Shohei Mori   +2 more
doaj   +1 more source

A Widely Applicable Urea-based Fluorescent/Colorimetric mRNA in situ Hybridization Protocol

open access: yesBio-Protocol, 2019
In situ hybridization methods are routinely employed to detect nucleic acid sequences, allowing to localize gene expression or to study chromosomal organization in their native context. These methods rely on the pairwise binding of a labeled probe to the
Chiara Sinigaglia
doaj   +1 more source

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