Results 31 to 40 of about 323,426 (336)

Microgels and apparatus for PAGE of nucleic acids in one or two dimensions

BioTechniques, 2018
We describe a system for horizontal 1D or 2D PAGE comprising an apparatus and microgels. There is no buffer outside the gel, making handling and sample loading easy.
Hans G Thormar, Gudmundur H Gunnarsson, Bjarki Gudmundsson, Kristjan Leosson, Peter Estibeiro, Jon J Jonsson   +5 more
doaj   +1 more source

DNA Renaturation at the Water-Phenol Interface [PDF]

, 2003
We study DNA adsorption and renaturation in a water-phenol two-phase system, with or without shaking. In very dilute solutions, single-stranded DNA is adsorbed at the interface in a salt-dependent manner.
121, 125, 23, 262, 27, 276, 28, 289, 35, 376, 38, 381, 395, 41, 52, 56, 7, 740, 77, 82, 90, 90, 98, 98, Alberts, Anderson, Anderson, Belhachemi, Berg, Berg, Bernal, Brahms, Braun, Campell, Carri, Carrington, Cech, Chan, Chaperon, Chomczynski, Chow, Christiansen, Craig, Davis, Deamer, DiMarzio, Duckett, Eigen, Eigen, Eisenberg, Escara, Felsenfeld, Fleming, Gennes, Gierer, Gilbert, Gold, Grassmann, Grosberg, Hagenmuller, Hammes, Hearst, Hegner, Helmer, Herschlag, Herskovits, Hill, Jaeger, Joanicot, Joyce, Joyce, Joyce, Katcher, Kauzmann, Kirby, Kirby, Kirby, Kohne, Kowalczykowski, Lahav, Lang, Laurent, Lennard-Jones, Leo, Lerman, Levine, Lohman, Ma, Maier, Maier, Mandell, Manning, Marmur, Marmur, Massie, Meijering, Meuthen, Miller, Morimatsu, Nadassi, Nandi, Neidle, Noller, Oparin, Pace, Pace, Paecht-Horowitz, Papafil, Pelta, Perutz, Piechowska, Piechowska, Pontius, Prigogine, Pusztai, Pörschke, Rhodes, Richter, Riggs, Robertson, Rothman, Saenger, Saito, Saxinger, Schaper, Schürmann, Sevag, Sikorav, Singer, Sinsheimer, Skinner, Smith, Smoluchowski, Studier, Studier, Szostak, Tabor, Tikhonenko, Timmermans, Tor, Tsang, Tsuchihashi, Ts’o, Ts’o, Vesnaver, Wagner, Wang, Ward, Weinstock, Westhof, Wetmur, Wetmur, Widom, Wieder, Winter, Woese, Wolfe, Zaks, Zaks, Zasloff, Zhulina, Ölçer   +161 more
core   +3 more sources

Labeling of Unique Sequences in Double-Stranded DNA at Sites of Vicinal Nicks Generated by Nicking Endonucleases [PDF]

, 2008
We describe a new approach for labeling of unique sequences within dsDNA under nondenaturing conditions. The method is based on the site-specific formation of vicinal nicks, which are created by nicking endonucleases (NEases) at specified DNA sites on ...
Frank-Kamenetskii, Maxim D., Kuhn, Heiko
core   +3 more sources

C5-Azobenzene-substituted 2'-Deoxyuridine-containing Oligodeoxynucleotides for Photo-Switching Hybridization

Molecules, 2014
A new photoisomeric nucleoside dUAz bearing an azobenzene group at the C5-position of 2'-deoxyuridine was designed and synthesized. Photoisomerization of dUAz in oligodeoxynucleotides can be achieved rapidly and selectively with 365 nm (forward) and 450
Shohei Mori, Kunihiko Morihiro, Satoshi Obika   +2 more
doaj   +1 more source

A Widely Applicable Urea-based Fluorescent/Colorimetric mRNA in situ Hybridization Protocol

Bio-Protocol, 2019
In situ hybridization methods are routinely employed to detect nucleic acid sequences, allowing to localize gene expression or to study chromosomal organization in their native context. These methods rely on the pairwise binding of a labeled probe to the
Chiara Sinigaglia
doaj   +1 more source

Rubredoxin Variant Folds without Iron [PDF]

, 1999
Pyroccocus furiosus rubredoxin (PFRD), like most studied hyperthermophilic proteins, does not undergo reversible folding. The irreversibility of folding is thought to involve PFRD’s iron-binding site.
Mayo, Stephen L., Strop, Pavel
core   +1 more source

Measurement of Epstein-Barr virus DNA load using a novel quantification standard containing two EBV DNA targets and SYBR Green I dye [PDF]

, 2016
BACKGROUND Reactivation of Epstein-Barr virus (EBV) infection may cause serious, life-threatening complications in immunocompromised individuals. EBV DNA is often detected in EBV-associated disease states, with viral load believed to be a reflection of ...
Ausimmune Investigator Group (AIG), Dwyer, Dominic E, Lay, Meav-Lang J, Lucas, Robyn, Ponsonby, Anne-Louise, Ratnamohan, Mala, Taylor, Janette   +6 more
core   +1 more source

Structure, kinetic characterization and subcellular localization of the two ribulose 5-phosphate epimerase isoenzymes from Trypanosoma cruzi [PDF]

, 2017
The enzyme of the pentose phosphate pathway (PPP) ribulose-5-phosphate-epimerase (RPE) is encoded by two genes present in the genome of Trypanosoma cruzi CL Brener clone: TcRPE1 and TcRPE2. Despite high sequence similarity at the amino acid residue level,
Cazzulo, Juan Jose, Delfino, Jose Maria, Gonzalez, Soledad Natalia, Maugeri, Dante, Valsecchi, Wanda Mariela   +4 more
core   +11 more sources

Microfluidic-Based Amplification-Free Bacterial DNA Detection by Dielectrophoretic Concentration and Fluorescent Resonance Energy Transfer Assisted in Situ Hybridization (FRET-ISH)

Biosensors, 2012
Although real-time PCR (RT-PCR) has become a diagnostic standard for rapid identification of bacterial species, typical methods remain time-intensive due to sample preparation and amplification cycle times.
Maxim Shusteff, Evangelyn C. Alocilja, Michelle M. Packard   +2 more
doaj   +1 more source

Effects of Chemical Sterilization and Gamma Irradiation on the Biochemical and Biomechanical Properties of Human Tendon Allografts In Vitro Study

Orthopaedic Surgery, 2022
Objective Pre‐implantation sterilization procedures for tendons are important measures to reduce the risk of disease transmission, however these procedures may compromise tendon microarchitecture and biomechanical properties to varying degrees.
Hao‐ran Zhang, Ming‐you Xu, Lei Zhang, Hao Zhang, Li Yang, Jie Liu, Jing‐yu Zhang, Yong‐cheng Hu   +7 more
doaj   +1 more source