Studies on Nucleic Acid Reassociation Kinetics: Retarded Rate of Hybridization of RNA with Excess DNA [PDF]
, 1977 The rate of reaction of excess double-stranded bacteriophage phi X174 and plasmid RSF2124 DNA drivers with enzymatically synthesized asymmetric RNA tracers was measured. Other reactions were carried out with excess Escherichia coli DNA and E.
Britten, Roy J. +3 more
core +1 more source
Brush Effects on DNA Chips: Thermodynamics, Kinetics and Design Guidlines [PDF]
, 2005 In biology experiments, oligonucleotide microarrays are contacted with a solution of long nucleic acid (NA) targets. The hybridized probes thus carry long tails. When the surface density of the oligonucleotide probes is high enough, the progress of hybridization leads to the formation of a polyelectrolyte brush due to mutual crowding of the NA tails ...
arxiv +1 more source
DNA crosslinking and biological activity of a hairpin polyamide–chlorambucil conjugate [PDF]
, 2003 A prototype of a novel class of DNA alkylating agents, which combines the DNA crosslinking moiety chlorambucil (Chl) with a sequence-selective hairpin pyrrole-imidazole polyamide ImPy-beta-ImPy-gamma-ImPy-beta-Dp (polyamide 1), was evaluated for its ...
Beerman, Terry A. +5 more
core +2 more sources
Molecular structure and interactions of nucleic acid components in nanopaticles: ab initio calculations [PDF]
Ukrainian Journal of Physics, 2012, Vol. 57, no. 7, pp. 723-731, 2012 Self-associates of nucleic acid components (stacking trimers and tetramers of the base pairs of nucleic acids) and short fragments of nucleic acids are nanoparticles (linear sizes of these particles are more than 10 A. Modern quantum-mechanical methods and softwares allow one to perform ab initio calculations of the systems consisting of 150-200 atoms ...
arxiv
Role of Proteome Physical Chemistry in Cell Behavior. [PDF]
, 2016 We review how major cell behaviors, such as bacterial growth laws, are derived from the physical chemistry of the cell's proteins. On one hand, cell actions depend on the individual biological functionalities of their many genes and proteins.
de Graff, Adam MR +3 more
core +3 more sources
Urea destabilizes RNA by forming stacking interactions and multiple hydrogen bonds with nucleic acid bases [PDF]
J. Am. Chem. Soc. (2009) vol.131, 17759-17761, 2009 Urea titration of RNA by urea is an effective approach to investigate the forces stabilizing this biologically important molecule. We used all atom molecular dynamics simulations using two urea force fields and two RNA constructs to elucidate in atomic detail the destabilization mechanism of folded RNA in aqueous urea solutions.
arxiv
Bio-inspired fabrication of DNA-inorganic hybrid composites using synthetic DNA [PDF]
, 2019 Nucleic acid nanostructures have attracted significant interest as potential therapeutic and diagnostic platforms due to their intrinsic biocompatibility and biodegradability, structural and functional diversity, and compatibility with various ...
Argawal, S +6 more
core +3 more sources
Flexibility of nucleic acids: from DNA to RNA [PDF]
arXiv, 2015 The structural flexibility of nucleic acids plays a key role in many fundamental life processes, such as gene replication and expression, DNA-protein recognition, and gene regulation. To obtain a thorough understanding of nucleic acid flexibility, extensive studies have been performed using various experimental methods and theoretical models.
arxiv
Immobilization of single strand DNA on solid substrate
, 2008 Thin films based on Layer-by-Layer (LbL) self assembled technique are useful for immobilization of DNA onto solid support. This communication reports the immobilization of DNA onto a solid support by electrostatic interaction with a polycation Poly ...
Bhowmik +25 more
core +1 more source
Flexibility-Rigidity Index for Protein-Nucleic Acid Flexibility and Fluctuation Analysis [PDF]
arXiv, 2015 Protein-nucleic acid complexes are important for many cellular processes including the most essential function such as transcription and translation. For many protein-nucleic acid complexes, flexibility of both macromolecules has been shown to be critical for specificity and/or function. Flexibility-rigidity index (FRI) has been proposed as an accurate
arxiv