High-Resolution Detection of Loss of Heterozygosity of Dinucleotide Microsatellite Markers
BioTechniques, 2001 Dinucleotide microsatellite markers are frequently investigated to study inheritance, genetic stability, and allele frequency distribution in a wide variety of genetic disorders. Previous studies have encountered significant problems regarding resolution
Rebecca N. Hourihan +2 more
doaj +1 more source
A conformational RNA zipper promotes intron ejection during non-conventional XBP1 mRNA splicing. [PDF]
, 2015 The kinase/endonuclease IRE1 is the most conserved signal transducer of the unfolded protein response (UPR), an intracellular signaling network that monitors and regulates the protein folding capacity of the endoplasmic reticulum (ER).
Acosta-Alvear, Diego +3 more
core +1 more source
Cover Picture: Locked Nucleic Acids and Intercalating Nucleic Acids in the Design of Easily Denaturing Nucleic Acids: Thermal Stability Studies (ChemBioChem 12/2004) [PDF]
ChemBioChem, 2004 The cover picture shows fully modified locked nucleic acids (LNAs, orange) and intercalating nucleic acids (INAs®, red) with several insertions of (R)‐1‐O‐(1‐pyrenylmethyl)glycerol, both displaying higher binding affinity to native DNA (white) than to each other. In their full paper on p. 1673 ff, E. B. Pedersen et al.
B. Ravindra Babu +4 more
openaire +2 more sources
Human PrimPol mutation associated with high myopia has a DNA replication defect [PDF]
, 2014 PrimPol is a primase-polymerase found in humans, and other eukaryotes, involved in bypassing lesions encountered during DNA replication. PrimPol employs both translesion synthesis and repriming mechanisms to facilitate lesion bypass by the replisome ...
Aguilera +27 more
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Thermal Stability of Peptide Nucleic Acid Complexes.
Journal of Physical Chemistry B, 2019 Peptide nucleic acid (PNA) is a neutral nucleic acid analogue that base pairs with itself and natural nucleic acids. PNA-nucleic acid complexes are more thermally stable than the corresponding complexes of natural nucleic acids.
M. Jasiński +3 more
semanticscholar +1 more source
Development of a nucleic acid lateral flow immunoassay for simultaneous detection of Listeria spp. and Listeriamonocytogenes in food [PDF]
, 2009 We present a new nucleic acid lateral flow immunoassay (NALFIA) for the assessment of listeria contamination. The detection procedure starts with enrichment of sample in Half Fraser broth (24 h). Following isolation of DNA, a duplex PCR is performed with
Amerongen, A., van +3 more
core +2 more sources
Analytical SciencesThe escalating threat of emerging diseases, often stemming from contaminants and lethal pathogens, has precipitated a heightened demand for sophisticated diagnostic tools. Within this landscape, the functional nucleic acid (FNA) biosensor, harnessing the
David Septian Sumanto Marpaung +3 more
semanticscholar +1 more source
Atomistic simulations of RNA duplex thermal denaturation: sequence- and forcefield-dependence
bioRxiv, 2023 Double-stranded RNA is the end-product of template-based replication, and is also the functional state of some biological RNAs. Similarly to proteins and DNA, they can be denatured by temperature, with important physiological and technological ...
Aimeric Dabin, Guillaume Stirnemann
semanticscholar +1 more source
Can we model DNA at the mesoscale ? Comment on: Fluctuations in the DNA double helix: A critical review [PDF]
, 2014 Comment on "Fluctuations in the DNA double helix: A critical review" by Frank-Kamenetskii and ...
Dauxois, Thierry, Peyrard, Michel
core +4 more sources
PCR performance of a thermostable heterodimeric archaeal DNA polymerase
Frontiers in Microbiology, 2014 DNA polymerases are versatile tools used in numerous important molecular biological core technologies like the ubiquitous polymerase chain reaction (PCR), cDNA cloning, genome sequencing and nucleic acid based diagnostics.
Tom eKillelea +3 more
doaj +1 more source