Results 41 to 50 of about 252,695 (308)
Orthopaedic Surgery, 2022 Objective Pre‐implantation sterilization procedures for tendons are important measures to reduce the risk of disease transmission, however these procedures may compromise tendon microarchitecture and biomechanical properties to varying degrees.
Hao‐ran Zhang +7 more
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Biosensors, 2012 Although real-time PCR (RT-PCR) has become a diagnostic standard for rapid identification of bacterial species, typical methods remain time-intensive due to sample preparation and amplification cycle times.
Maxim Shusteff +2 more
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Cover Picture: Locked Nucleic Acids and Intercalating Nucleic Acids in the Design of Easily Denaturing Nucleic Acids: Thermal Stability Studies (ChemBioChem 12/2004) [PDF]
ChemBioChem, 2004 The cover picture shows fully modified locked nucleic acids (LNAs, orange) and intercalating nucleic acids (INAs®, red) with several insertions of (R)‐1‐O‐(1‐pyrenylmethyl)glycerol, both displaying higher binding affinity to native DNA (white) than to each other. In their full paper on p. 1673 ff, E. B. Pedersen et al.
Vyacheslav V. Filichev +4 more
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High-Resolution Detection of Loss of Heterozygosity of Dinucleotide Microsatellite Markers
BioTechniques, 2001 Dinucleotide microsatellite markers are frequently investigated to study inheritance, genetic stability, and allele frequency distribution in a wide variety of genetic disorders. Previous studies have encountered significant problems regarding resolution
Rebecca N. Hourihan +2 more
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Easily denaturing nucleic acids derived from intercalating nucleic acids: thermal stability studies, dual duplex invasion and inhibition of transcription start [PDF]
Nucleic Acids Research, 2005 The bulged insertions of (R)-1-O-(pyren-1-ylmethyl)glycerol (monomer P) in two complementary 8mer DNA strands (intercalating nucleic acids) opposite to each other resulted in the formation of an easily denaturing duplex, which had lower thermal stability (21.0 degrees C) than the wild-type double-stranded DNA (dsDNA, 26.0 degrees C), but both modified ...
Filichev, Vyacheslav V +3 more
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Thermal Stability of Peptide Nucleic Acid Complexes.
Journal of Physical Chemistry B, 2019 Peptide nucleic acid (PNA) is a neutral nucleic acid analogue that base pairs with itself and natural nucleic acids. PNA-nucleic acid complexes are more thermally stable than the corresponding complexes of natural nucleic acids.
M. Jasiński +3 more
semanticscholar +1 more source
Development of a combined diphtheria vaccine based on diphtheria anatoxin with microbial adjuvant in the light of modern strategies of vaccines development [PDF]
Annals of Mechnikov's Institute, 2020 The review article is devoted to the consideration of approaches to the practical implementation of the main provisions of modern ideas about the role and mechanisms of innate immunity for the development of vaccines.
Yelyseyeva, I.V. +4 more
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PCR performance of a thermostable heterodimeric archaeal DNA polymerase
Frontiers in Microbiology, 2014 DNA polymerases are versatile tools used in numerous important molecular biological core technologies like the ubiquitous polymerase chain reaction (PCR), cDNA cloning, genome sequencing and nucleic acid based diagnostics.
Tom eKillelea +3 more
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Journal of Functional Biomaterials, 2012 Biopolymers are easily denatured by heating, a change in pH or chemical substances when they are immobilized on a substrate. To prevent denaturation of biopolymers, we developed a method to trap a polynucleotide on a substrate by hydrogen bonding using ...
R. Maeda, T. Isoda
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A Rapid and Low-Cost PCR Thermal Cycler for Low Resource Settings. [PDF]
PLoS ONE, 2015 Many modern molecular diagnostic assays targeting nucleic acids are typically confined to developed countries or to the national reference laboratories of developing-world countries.
Grace Wong +4 more
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