Results 1 to 10 of about 316,767 (328)
Continuously tunable nucleic acid hybridization probes. [PDF]
Nat Methods, 2015 In silico-designed nucleic acid probes and primers often do not achieve favorable specificity and sensitivity tradeoffs on the first try, and iterative empirical sequence-based optimization is needed, particularly in multiplexed assays. We present a novel, on-the-fly method of tuning probe affinity and selectivity by adjusting the stoichiometry of ...
Wu LR +10 more
europepmc +8 more sources
Optimizing the specificity of nucleic acid hybridization. [PDF]
Nat Chem, 2012 The specific hybridization of complementary sequences is an essential property of nucleic acids, enabling diverse biological and biotechnological reactions and functions. However, the specificity of nucleic acid hybridization is compromised for long strands, except near the melting temperature. Here, we analytically derived the thermodynamic properties
Zhang DY, Chen SX, Yin P.
europepmc +6 more sources
Colorimetric Detection of Ehrlichia Canis via Nucleic Acid Hybridization in Gold Nano-Colloids [PDF]
Sensors, 2014 Canine monocytic ehrlichiosis (CME) is a major thick-bone disease of dog caused by Ehrlichia canis. Detection of this causal agent outside the laboratory using conventional methods is not effective enough. Thus an assay for E.
Ajima Muangchuen +3 more
doaj +4 more sources
Photoinitiator nucleotide for quantifying nucleic Acid hybridization. [PDF]
Biomacromolecules, 2010 This first report of a photoinitiator-nucleotide conjugate demonstrates a novel approach for sensitive, rapid and visual detection of DNA hybridization events. This approach holds potential for various DNA labeling schemes and for applications benefiting from selective DNA-based polymerization initiators.
Johnson LM +4 more
europepmc +6 more sources
Surface Micro-Patterned Biofunctionalized Hydrogel for Direct Nucleic Acid Hybridization Detection [PDF]
Biosensors, 2023 The present research is focused on the development of a biofunctionalized hydrogel with a surface diffractive micropattern as a label-free biosensing platform.
Paola Zezza +4 more
doaj +2 more sources
Topological constraints in nucleic acid hybridization kinetics [PDF]
Nucleic Acids Research, 2005 A theoretical examination of kinetic mechanisms for forming knots and links in nucleic acid structures suggests that molecules involving base pairs between loops are likely to become topologically trapped in persistent frustrated states through the mechanism of 'helix-driven wrapping'. Augmentation of the state space to include both secondary structure
Justin S. Bois
openalex +6 more sources
BioTechniques, 1999 We optimized a novel nonradioactive hybridization technique using a cis-platin coupled digoxigenin derivative for direct labeling of mRNA. This new mRNA reporter molecule was applied to cDNA membrane arrays to simultaneously identify expression of ...
T. Hoevel, H. Holz, M. Kubbies
doaj +2 more sources
Fast and non-toxic in situ hybridization without blocking of repetitive sequences. [PDF]
PLoS ONE, 2012 Formamide is the preferred solvent to lower the melting point and annealing temperature of nucleic acid strands in in situ hybridization (ISH). A key benefit of formamide is better preservation of morphology due to a lower incubation temperature. However,
Steen H Matthiesen, Charles M Hansen
doaj +1 more source
PLoS ONE, 2022 The ability to predict nucleic acid hybridization energies has been greatly enabling for many applications, but predictive models require painstaking experimentation, which may limit expansion to non-natural nucleic acid analogues and chemistries.
Siyuan S Wang +3 more
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Application of Graphene FET Nucleic Acid Biosensor in Human Motion Measurement [PDF]
International Journal Bioautomation, 2018 In order to explore the application of graphene field effect transistor (FET) nucleic acid biosensor, the sensitivity and selectivity of nano FET biosensor detection are improved by the large physical surface ratio, high electron mobility, excellent ...
Hang Yin
doaj +1 more source