Results 1 to 10 of about 110,082 (88)
Nucleic acid analysis by sandwich hybridization
Journal of Clinical Laboratory Analysis, 1989 AbstractOne of the most significant achievements of the biochemist during the past two decades is the use to which immunologically based assays have been put in clinical diagnosis (Hood et al.: Immunology, 1984). The problem faced and surmounted by immunologists in effecting the transition from research tool to routine clinical assay bears a remarkable
Peter Nicholls, Alan D. B. Malcolm
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The effects of mismatches on hybridization in DNA microarrays: determination of nearest neighbor parameters [PDF]
, 2010 Quantifying interactions in DNA microarrays is of central importance for a better understanding of their functioning. Hybridization thermodynamics for nucleic acid strands in aqueous solution can be described by the so-called nearest-neighbor model ...
Bloomfield +23 more
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Fluorescent labeling of plasmid DNA and mRNA : gains and losses of current labeling strategies [PDF]
, 2016 Live-cell imaging has provided the life sciences with insights into the cell biology and dynamics. Fluorescent labeling of target molecules proves to be indispensable in this regard. In this Review, we focus on the current fluorescent labeling strategies
Rombouts, Koen +2 more
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Electrically Guided DNA Immobilization and Multiplexed DNA Detection with Nanoporous Gold Electrodes. [PDF]
, 2018 Molecular diagnostics have significantly advanced the early detection of diseases, where the electrochemical sensing of biomarkers (e.g., DNA, RNA, proteins) using multiple electrode arrays (MEAs) has shown considerable promise.
Daggumati, Pallavi +3 more
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Electron microscopic visualization of tRNA genes with ferritin-avidin: biotin labels [PDF]
, 1978 A method is described for indirect electron microscopic visualization and mapping of tRNA and other short transcripts hybridized to DNA. This method depends upon the attachment of the electron-dense protein ferritin to the RNA, the binding being mediated
Angerer, Lynne M. +4 more
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CARD In Situ hybridization: Sights and signals [PDF]
, 2018 During the last decade, several strategies have been developed to improve the detection sensitivity ofin situ hybridization (ISH) by amplification of either target nucleic acid sequences prior to ISH (e.g.,in situ PCR), or the detection signals after the
Komminoth, Paul, Speel, Ernst
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Studies on Nucleic Acid Reassociation Kinetics: Retarded Rate of Hybridization of RNA with Excess DNA [PDF]
, 1977 The rate of reaction of excess double-stranded bacteriophage phi X174 and plasmid RSF2124 DNA drivers with enzymatically synthesized asymmetric RNA tracers was measured. Other reactions were carried out with excess Escherichia coli DNA and E.
Britten, Roy J. +3 more
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, 2020 Abbreviations -- Preface -- 1. Introduction to nucleic acid hybridization -- Structure of nucleic acids -- Forces stabilizing DNA structure -- Mismatched base pairs -- Stability of nucleic acids -- Effects of pH -- Effects of temperature -- Renaturation and hybridization -- 2.
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Hybrids of Nucleic Acids and Carbon Nanotubes for Nanobiotechnology [PDF]
Nanomaterials, 2015 Recent progress in the combination of nucleic acids and carbon nanotubes (CNTs) has been briefly reviewed here. Since discovering the hybridization phenomenon of DNA molecules and CNTs in 2003, a large amount of fundamental and applied research has been carried out.
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Method for amplifying nucleic acids [PDF]
, 2012 The present invention describes methods for amplifying a target nucleic acid wherein target nucleic acids hybridise to capture probe nucleic acids which are immobilized to a support via their 5 ' end.
Feitsma, Harma Martine +3 more
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