Surface Micro-Patterned Biofunctionalized Hydrogel for Direct Nucleic Acid Hybridization Detection [PDF]
Biosensors, 2023 The present research is focused on the development of a biofunctionalized hydrogel with a surface diffractive micropattern as a label-free biosensing platform.
Paola Zezza +4 more
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Continuously tunable nucleic acid hybridization probes. [PDF]
Nat Methods, 2015 In silico-designed nucleic acid probes and primers often do not achieve favorable specificity and sensitivity tradeoffs on the first try, and iterative empirical sequence-based optimization is needed, particularly in multiplexed assays. We present a novel, on-the-fly method of tuning probe affinity and selectivity by adjusting the stoichiometry of ...
Wu LR +10 more
europepmc +5 more sources
Activation of enzymatic catalysis via nucleic acid hybridization affords synergistic coupling of specificity, potency, and signal amplification [PDF]
Nature CommunicationsIn this work, we establish the use of nucleic acid hybridization to program the activation of catalysis in enzymes. We do this via “thiol switching”, using thiolated oligonucleotides: a protein is inactivated by conjugation to an oligonucleotide via a ...
Mireia Casanovas Montasell +4 more
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Label-Free Potentiometry for Detecting DNA Hybridization Using Peptide Nucleic Acid and DNA Probes [PDF]
Sensors, 2013 Peptide nucleic acid (PNA) has outstanding affinity over DNA for complementary nucleic acid sequences by forming a PNA-DNA heterodimer upon hybridization via Watson-Crick base-pairing.
Yuji Miyahara +6 more
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Optimizing the specificity of nucleic acid hybridization. [PDF]
Nat Chem, 2012 The specific hybridization of complementary sequences is an essential property of nucleic acids, enabling diverse biological and biotechnological reactions and functions. However, the specificity of nucleic acid hybridization is compromised for long strands, except near the melting temperature. Here, we analytically derived the thermodynamic properties
Zhang DY, Chen SX, Yin P.
europepmc +4 more sources
Colorimetric Detection of Ehrlichia Canis via Nucleic Acid Hybridization in Gold Nano-Colloids [PDF]
Sensors, 2014 Canine monocytic ehrlichiosis (CME) is a major thick-bone disease of dog caused by Ehrlichia canis. Detection of this causal agent outside the laboratory using conventional methods is not effective enough. Thus an assay for E.
Ajima Muangchuen +3 more
doaj +2 more sources
Enzyme fragment complementation driven by nucleic acid hybridization sans self-labeling protein. [PDF]
Bioorg ChemXu Z +4 more
europepmc +3 more sources
Photoinitiator nucleotide for quantifying nucleic Acid hybridization. [PDF]
Biomacromolecules, 2010 This first report of a photoinitiator-nucleotide conjugate demonstrates a novel approach for sensitive, rapid and visual detection of DNA hybridization events. This approach holds potential for various DNA labeling schemes and for applications benefiting from selective DNA-based polymerization initiators.
Johnson LM +4 more
europepmc +4 more sources
Nucleic acid amphiphiles: Synthesis, properties, and applications
Molecular Therapy: Nucleic Acids, 2023 Nucleic acid amphiphiles, referring to nucleic acids modified with large hydrophobic groups, have been widely used in programmable bioengineering. Since nucleic acids are intrinsically hydrophilic, the hydrophobic groups endow nucleic acid amphiphiles ...
Amu Gubu +5 more
doaj +1 more source
Fast and non-toxic in situ hybridization without blocking of repetitive sequences. [PDF]
PLoS ONE, 2012 Formamide is the preferred solvent to lower the melting point and annealing temperature of nucleic acid strands in in situ hybridization (ISH). A key benefit of formamide is better preservation of morphology due to a lower incubation temperature. However,
Steen H Matthiesen, Charles M Hansen
doaj +1 more source