Results 11 to 20 of about 225,030 (341)
Photoinitiator nucleotide for quantifying nucleic Acid hybridization. [PDF]
Biomacromolecules, 2010 This first report of a photoinitiator-nucleotide conjugate demonstrates a novel approach for sensitive, rapid and visual detection of DNA hybridization events. This approach holds potential for various DNA labeling schemes and for applications benefiting from selective DNA-based polymerization initiators.
Johnson LM +4 more
europepmc +4 more sources
Nucleic acid amphiphiles: Synthesis, properties, and applications
Molecular Therapy: Nucleic Acids, 2023 Nucleic acid amphiphiles, referring to nucleic acids modified with large hydrophobic groups, have been widely used in programmable bioengineering. Since nucleic acids are intrinsically hydrophilic, the hydrophobic groups endow nucleic acid amphiphiles ...
Amu Gubu +5 more
doaj +1 more source
Fast and non-toxic in situ hybridization without blocking of repetitive sequences. [PDF]
PLoS ONE, 2012 Formamide is the preferred solvent to lower the melting point and annealing temperature of nucleic acid strands in in situ hybridization (ISH). A key benefit of formamide is better preservation of morphology due to a lower incubation temperature. However,
Steen H Matthiesen, Charles M Hansen
doaj +1 more source
PLoS ONE, 2022 The ability to predict nucleic acid hybridization energies has been greatly enabling for many applications, but predictive models require painstaking experimentation, which may limit expansion to non-natural nucleic acid analogues and chemistries.
Siyuan S Wang +3 more
doaj +1 more source
Application of Graphene FET Nucleic Acid Biosensor in Human Motion Measurement [PDF]
International Journal Bioautomation, 2018 In order to explore the application of graphene field effect transistor (FET) nucleic acid biosensor, the sensitivity and selectivity of nano FET biosensor detection are improved by the large physical surface ratio, high electron mobility, excellent ...
Hang Yin
doaj +1 more source
Protein-Based Anchoring Methods for Nucleic Acid Detection in Lateral Flow Format Assays
Micromachines, 2023 The use of lateral flow assays to detect nucleic acid targets has many applications including point-of-care diagnostics, environmental monitoring, and food safety.
Kira Hallerbach +6 more
doaj +1 more source
Highly efficient and robust π-FISH rainbow for multiplexed in situ detection of diverse biomolecules
Nature Communications, 2023 Fluorescence in situ hybridization (FISH) methods with high sensitivity are needed. Here the authors develop multiplex πFISH rainbow to detect a range of biomolecules; they also combine this with the hybridization chain reaction to develop πFISH ...
Yingfeng Tao +17 more
doaj +1 more source
Nucleic Acid Hybridization [PDF]
, 2020 Abbreviations -- Preface -- 1. Introduction to nucleic acid hybridization -- Structure of nucleic acids -- Forces stabilizing DNA structure -- Mismatched base pairs -- Stability of nucleic acids -- Effects of pH -- Effects of temperature -- Renaturation and hybridization -- 2.
openaire +3 more sources
Exploiting double exchange Diels-Alder cycloadditions for immobilization of peptide nucleic acids on gold nanoparticles [PDF]
, 2020 The generation of PNA-decorated gold nanoparticles (AuNPs) has revealed to be more difficult as compared to the generation of DNA-functionalized ones. The less polar nature of this artificial nucleic acid system and the associated tendency of the neutral
Cadoni, Enrico +4 more
core +2 more sources
Nucleic acid nanoassembly-enhanced RNA therapeutics and diagnosis
Acta Pharmaceutica Sinica B, 2023 RNAs are involved in the crucial processes of disease progression and have emerged as powerful therapeutic targets and diagnostic biomarkers. However, efficient delivery of therapeutic RNA to the targeted location and precise detection of RNA markers ...
Mengnan Zhao +8 more
doaj +1 more source