Definitions, 2020 Abbreviations -- Preface -- 1. Introduction to nucleic acid hybridization -- Structure of nucleic acids -- Forces stabilizing DNA structure -- Mismatched base pairs -- Stability of nucleic acids -- Effects of pH -- Effects of temperature -- Renaturation and hybridization -- 2.
M. Anderson
openaire +4 more sources
A quantitative model predicts how m<sup>6</sup>A reshapes the kinetic landscape of nucleic acid hybridization and conformational transitions. [PDF]
Nat Commun, 2021 N6-methyladenosine (m6A) is a post-transcriptional modification that controls gene expression by recruiting proteins to RNA sites. The modification also slows biochemical processes through mechanisms that are not understood.
Liu B +8 more
europepmc +2 more sources
Label-Free Potentiometry for Detecting DNA Hybridization Using Peptide Nucleic Acid and DNA Probes
Sensors, 2013 Peptide nucleic acid (PNA) has outstanding affinity over DNA for complementary nucleic acid sequences by forming a PNA-DNA heterodimer upon hybridization via Watson-Crick base-pairing.
Yuji Miyahara +6 more
doaj +2 more sources
Pharmacokinetic Profiling of Conjugated Therapeutic Oligonucleotides: A High-Throughput Method Based Upon Serial Blood Microsampling Coupled to Peptide Nucleic Acid Hybridization Assay. [PDF]
Nucleic Acid Ther, 2017 Therapeutic oligonucleotides, such as small interfering RNAs (siRNAs), hold great promise for the treatment of incurable genetically defined disorders by targeting cognate toxic gene products for degradation. To achieve meaningful tissue distribution and
Godinho BMDC +9 more
europepmc +2 more sources
Determination of nucleic acid sequence homologies and relative concentrations by a dot hybridization procedure [PDF]
Nucleic Acids Research, 1979 A dot hybridization method is presented for rapidly determining the relative concentrations of nucleic acids in a mixture, as well as the extent of sequence homology between related RNA or DNA species.
Fotis C. Kafatos +2 more
openalex +2 more sources
Complex Nucleic Acid Hybridization Reactions inside Capillary-Driven Microfluidic Chips.
Small, 2020 Nucleic acid hybridization reactions play an important role in many (bio)chemical fields, for example, for the development of portable point-of-care diagnostics, and often such applications require nucleic acid-based reaction systems that ideally run ...
Marie L. Salva +4 more
semanticscholar +1 more source
Highly efficient and robust π-FISH rainbow for multiplexed in situ detection of diverse biomolecules
Nature Communications, 2023 Fluorescence in situ hybridization (FISH) methods with high sensitivity are needed. Here the authors develop multiplex πFISH rainbow to detect a range of biomolecules; they also combine this with the hybridization chain reaction to develop πFISH ...
Yingfeng Tao +17 more
doaj +1 more source
Protein-Based Anchoring Methods for Nucleic Acid Detection in Lateral Flow Format Assays
Micromachines, 2023 The use of lateral flow assays to detect nucleic acid targets has many applications including point-of-care diagnostics, environmental monitoring, and food safety.
Kira Hallerbach +6 more
doaj +1 more source
Nucleic Acid Hybrids as Advanced Antibacterial Nanocarriers [PDF]
Pharmaceutics, 2020 Conventional antibiotic therapy is often challenged by poor drug penetration/accumulation at infection sites and poses a significant burden to public health. Effective strategies to enhance the therapeutic efficacy of our existing arsenal include the use of nanoparticulate delivery platforms to improve drug targeting and minimize adverse effects ...
Sybil Obuobi, Nataša Škalko-Basnet
openaire +5 more sources
Glycerol Reduces Cross Hybridization on Nitrocellulose Membrane
Jurnal Sain Veteriner, 2020 Lateral flow assay (LFD) based nucleic acid lateral flow (NALF) method has been developed recently. The method met point of care testing (POCT) as simple and rapid procedures, less equipment, and can be performance by less skilled technician. NALF based
Narendra Yoga Hendarta +3 more
doaj +1 more source