Furan-PNA : a mildly inducible irreversible interstrand crosslinking system targeting single and double stranded DNA [PDF]
, 2016 We here report on the design and synthesis of tailor-made furan-modified peptide nucleic acid (PNA) probes for covalent targeting of single stranded DNA through a crosslinking strategy.
Corradini, Roberto +3 more
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Topological constraints in nucleic acid hybridization kinetics [PDF]
Nucleic Acids Research, 2005 A theoretical examination of kinetic mechanisms for forming knots and links in nucleic acid structures suggests that molecules involving base pairs between loops are likely to become topologically trapped in persistent frustrated states through the mechanism of 'helix-driven wrapping'. Augmentation of the state space to include both secondary structure
Bois, Justin S. +5 more
openaire +5 more sources
Continuously tunable nucleic acid hybridization probes [PDF]
Nature Methods, 2015 In silico-designed nucleic acid probes and primers often do not achieve favorable specificity and sensitivity tradeoffs on the first try, and iterative empirical sequence-based optimization is needed, particularly in multiplexed assays. We present a novel, on-the-fly method of tuning probe affinity and selectivity by adjusting the stoichiometry of ...
Lucia R Wu +10 more
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Electrochemical Characterization of Regularly-aligned Nanopore Array Membranes Filled with Electrolyte Solutions and their use for Detection of Nucleic Acid Hybridization [PDF]
, 2011 We report on the electrochemical characterization of regularlyaligned cylindrical nanopore arrays supported in silicon nitride membranes and preliminary results for the detection of nucleic acid hybridization on the nanopore walls.
Arrigan, Damien +3 more
core +1 more source
An ultrasensitive photoelectrochemical nucleic acid biosensor [PDF]
, 2005 A simple and ultrasensitive procedure for non-labeling detection of nucleic acids is described in this study. It is based on the photoelectrochemical detection of target nucleic acids by forming a nucleic acid/photoreporter adduct layer on an ITO ...
Gao, Zhiqiang, Tansil, Natalia C.
core +3 more sources
Single-Molecule Imaging Reveals that Argonaute Reshapes the Binding Properties of Its Nucleic Acid Guides [PDF]
, 2015 SummaryArgonaute proteins repress gene expression and defend against foreign nucleic acids using short RNAs or DNAs to specify the correct target RNA or DNA sequence.
Jolly, Samson M. +4 more
core +1 more source
PCR-Independent Detection of Bacterial Species-Specific 16S rRNA at 10 fM by a Pore-Blockage Sensor. [PDF]
, 2016 A PCR-free, optics-free device is used for the detection of Escherichia coli (E. coli) 16S rRNA at 10 fM, which corresponds to ~100-1000 colony forming units/mL (CFU/mL) depending on cellular rRNA levels.
Banda, Anisha +7 more
core +1 more source
Real-time DNA microarray analysis [PDF]
, 2009 We present a quantification method for affinity-based DNA microarrays which is based on the real-time measurements of hybridization kinetics. This method, i.e.
Abdueva +56 more
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Hybridization properties of immobilized nucleic acids
Nucleic Acids Research, 1987 The 5'-end attachment of oligonucleotides to dextran supports facilitates the study of the hybridization properties of an immobilized oligonucleotide system. The hybridization properties which were studied include: hybridization capacity and kinetics, hybridization-complex stability, and reagents influencing hybridization efficiency.
G. R. Davis +2 more
openaire +4 more sources
Identification of antisense nucleic acid hybridization sites in mRNA molecules with self-quenching fluorescent reporter molecules [PDF]
, 2005 We describe a physical mRNA mapping strategy employing fluorescent self-quenching reporter molecules (SQRMs) that facilitates the identification of mRNA sequence accessible for hybridization with antisense nucleic acids in vitro and in vivo, real time ...
Do, Bao T. +11 more
core +2 more sources