Results 21 to 30 of about 114,253 (306)

Microfluidic Chip for the Electrochemical Detection of MicroRNAs: Methylene Blue Increasing the Specificity of the Biosensor

Frontiers in Chemistry, 2022
MicroRNAs (miRNAs) are biomarkers involved in biological processes that are released by cells and found in biological fluids such as blood. The development of nucleic acid-based biosensors has significantly increased in the past 10 years because the ...
Claire Poujouly, Jérémy Le Gall, Martina Freisa, Djamila Kechkeche, David Bouville, Jihed Khemir, Pedro Gonzalez-Losada, Jean Gamby   +7 more
doaj   +1 more source

Chemical Architecture and Applications of Nucleic Acid Derivatives Containing 1,2,3-Triazole Functionalities Synthesized via Click Chemistry

Molecules, 2012
There is considerable attention directed at chemically modifying nucleic acids with robust functional groups in order to alter their properties. Since the breakthrough of copper-assisted azide-alkyne cycloadditions (CuAAC), there have been several ...
Wei Gong, Tim Efthymiou, Jean-Paul Desaulniers   +2 more
doaj   +1 more source

A Study of the Detection of SARS-CoV-2 ORF1ab Gene by the Use of Electrochemiluminescent Biosensor Based on Dual-Probe Hybridization

Sensors, 2022
To satisfy the need to develop highly sensitive methods for detecting the severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2) and further enhance detection efficiency and capability, a new method was created for detecting SARS-CoV-2 of the ...
Chunying Jiang, Xihui Mu, Shuai Liu, Zhiwei Liu, Bin Du, Jiang Wang, Jianjie Xu   +6 more
doaj   +1 more source

Electropermeabilization-based fluorescence in situ hybridization of whole-mount plant parasitic nematode specimens

MethodsX, 2019
A fluorescence in situ hybridization (FISH) protocol was developed for nematodes in which nucleic acid probes are introduced within the organism via electroporation.
Casey L. Ruark-Seward, Eric L. Davis, Tim L. Sit   +2 more
doaj   +1 more source

A Widely Applicable Urea-based Fluorescent/Colorimetric mRNA in situ Hybridization Protocol

Bio-Protocol, 2019
In situ hybridization methods are routinely employed to detect nucleic acid sequences, allowing to localize gene expression or to study chromosomal organization in their native context. These methods rely on the pairwise binding of a labeled probe to the
Chiara Sinigaglia
doaj   +1 more source

Effect of Native Gastric Mucus on in vivo Hybridization Therapies Directed at Helicobacter pylori

Molecular Therapy: Nucleic Acids, 2015
Helicobacter pylori infects more than 50% of the worldwide population. It is mostly found deep in the gastric mucus lining of the stomach, being a major cause of peptic ulcers and gastric adenocarcinoma. To face the increasing resistance of H.
Rita S Santos, George R Dakwar, Ranhua Xiong, Katrien Forier, Katrien Remaut, Stephan Stremersch, Nuno Guimarães, Sílvia Fontenete, Jesper Wengel, Marina Leite, Céu Figueiredo, Stefaan C De Smedt, Kevin Braeckmans, Nuno F Azevedo   +13 more
doaj   +1 more source

Locked nucleic acid pentamers as universal PCR primers for genomic DNA amplification. [PDF]

PLoS ONE, 2008
BACKGROUND: Multiplexing technologies, which allow for simultaneous detection of multiple nucleic acid sequences in a single reaction, can save a lot of time, cost and labor compared to traditional single reaction detection methods.
Zhen Sun, Zhi Chen, Xiaoli Hou, Shuping Li, Haihong Zhu, Ji Qian, Daru Lu, Wei Liu   +7 more
doaj   +1 more source

Penggunaan Pelacak DNA untuk Deteksi Papaya ringspot virus dengan Metode Hibridisasi Asam Nukleat

Jurnal Fitopatologi Indonesia, 2018
Use of DNA Probe for Detection of Papaya ringspot virus Using Nucleic Acid Hybridization MethodPapaya ringspot caused by Papaya ringspot virus (PRSV) is one of the most destructive diseases of papaya.
Irsan Nuhantoro, Sri Hendrastuti Hidayat, Kikin Hamzah Mutaqin   +2 more
doaj   +1 more source

Thermodynamic framework to assess low abundance DNA mutation detection by hybridization. [PDF]

PLoS ONE, 2017
The knowledge of genomic DNA variations in patient samples has a high and increasing value for human diagnostics in its broadest sense. Although many methods and sensors to detect or quantify these variations are available or under development, the ...
Hanny Willems, An Jacobs, Wahyu Wijaya Hadiwikarta, Tom Venken, Dirk Valkenborg, Nadine Van Roy, Jo Vandesompele, Jef Hooyberghs   +7 more
doaj   +1 more source

Influence of citrate buffer and flash heating in enhancing the sensitivity of ratiometric genosensing of Hepatitis C virus using plasmonic gold nanoparticles

Micro and Nano Systems Letters, 2021
Gold nanoparticles (Au NPs) based technology has been shown to possess enormous potential in the viral nucleic acid diagnosis. Despite significant advancement in this domain, the existing literature reveals the diversity in the conditions employed for ...
Hrishikesh Shashi Prakash, Pranay Amruth Maroju, Naga Sai Sriteja Boppudi, Aniket Balapure, Ramakrishnan Ganesan, Jayati Ray Dutta   +5 more
doaj   +1 more source