Results 21 to 30 of about 225,030 (341)

Physico-chemical foundations underpinning microarray and next-generation sequencing experiments [PDF]

, 2013
Hybridization of nucleic acids on solid surfaces is a key process involved in high-throughput technologies such as microarrays and, in some cases, next-generation sequencing (NGS).
A. Buhot, A. E. Pozhitkov, A. Halperin, A. Harrison, A. Ott, Amend, B. M. Pettitt, Berger, Binder, Binder, Binder, Binder, Bolstad, Bullard, Burden, Burden, C. Gibas, C. J. Burden, Chou, Chou, Czypionka, D. P. Kreil, D. Tautz, E. Carlon, Fasold, Fasold, Fiche, Fuchs, H. Binder, Halperin, Harr, Harrison, He, Heim, Held, Hooyberghs, Huettel, Iltumur, Irizarry, Irizarry, Irizarry, Irving, J. Hooyberghs, Kane, L. J. Gamble, Lee, Lee, Letowski, Li, Liebich, Lockhart, Luebke, Marshall, Matveeva, Mueckstein, Mulders, Naiser, Naiser, P. A. Noble, Pingel, Pozhitkov, R. Levicky, Relogio, Rouillard, Tanaka, Trapp, Upton, Vainrub, Wodicka, Yu, Zhang   +70 more
core   +3 more sources

Nucleic Acid Analysis Using Multifunctional Hybridization Sensors

Proceedings, 2017
Hybridization of nucleic acid probes remains one of the most common strategies for sensing of [...]
Dmitry M. Kolpashchikov
doaj   +1 more source

Microfluidic Chip for the Electrochemical Detection of MicroRNAs: Methylene Blue Increasing the Specificity of the Biosensor

Frontiers in Chemistry, 2022
MicroRNAs (miRNAs) are biomarkers involved in biological processes that are released by cells and found in biological fluids such as blood. The development of nucleic acid-based biosensors has significantly increased in the past 10 years because the ...
Claire Poujouly, Jérémy Le Gall, Martina Freisa, Djamila Kechkeche, David Bouville, Jihed Khemir, Pedro Gonzalez-Losada, Jean Gamby   +7 more
doaj   +1 more source

Detection of fluorescence in situ hybridization on human metaphase chromosomes by near-field scanning optical microscopy [PDF]

, 1995
Fluorescence in situ hybridization signals o­n human metaphase chromosomes are detected by a near-field scanning optical microscope. This makes it possible to localize and identify several fluorescently labeled genomic DNA fragments o­n a single ...
Jalocha, A., Jalocha, A., Moers, M.H.P., Moers, M.H.P., Ruiter, A.G.T., van Hulst, N.F.   +5 more
core   +3 more sources

Nucleic Acid Hybrids as Advanced Antibacterial Nanocarriers [PDF]

Pharmaceutics, 2020
Conventional antibiotic therapy is often challenged by poor drug penetration/accumulation at infection sites and poses a significant burden to public health. Effective strategies to enhance the therapeutic efficacy of our existing arsenal include the use of nanoparticulate delivery platforms to improve drug targeting and minimize adverse effects ...
Sybil Obuobi, Nataša Škalko-Basnet
openaire   +4 more sources

A method for nucleic acid hybridization to isolated chromosomes in suspension [PDF]

, 1987
A procedure was developed to provide differential fluorescent staining of metaphase chromosomes in suspension following nucleic acid hybridization. For this purpose metaphase chromosomes were isolated from a Chinese hamster x human hybrid cell line ...
Bier, Frank, Cremer, Christoph, Cremer, Thomas, Dudin, Gertrud, Hausmann, Michael, Schardin, Margit   +5 more
core   +1 more source

A Study of the Detection of SARS-CoV-2 ORF1ab Gene by the Use of Electrochemiluminescent Biosensor Based on Dual-Probe Hybridization

Sensors, 2022
To satisfy the need to develop highly sensitive methods for detecting the severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2) and further enhance detection efficiency and capability, a new method was created for detecting SARS-CoV-2 of the ...
Chunying Jiang, Xihui Mu, Shuai Liu, Zhiwei Liu, Bin Du, Jiang Wang, Jianjie Xu   +6 more
doaj   +1 more source

Electropermeabilization-based fluorescence in situ hybridization of whole-mount plant parasitic nematode specimens

MethodsX, 2019
A fluorescence in situ hybridization (FISH) protocol was developed for nematodes in which nucleic acid probes are introduced within the organism via electroporation.
Casey L. Ruark-Seward, Eric L. Davis, Tim L. Sit   +2 more
doaj   +1 more source

Fluorescent labeling of plasmid DNA and mRNA : gains and losses of current labeling strategies [PDF]

, 2016
Live-cell imaging has provided the life sciences with insights into the cell biology and dynamics. Fluorescent labeling of target molecules proves to be indispensable in this regard. In this Review, we focus on the current fluorescent labeling strategies
Rombouts, Koen, Braeckmans, Kevin, Remaut, Katrien   +2 more
core   +1 more source

Electrically Guided DNA Immobilization and Multiplexed DNA Detection with Nanoporous Gold Electrodes. [PDF]

, 2018
Molecular diagnostics have significantly advanced the early detection of diseases, where the electrochemical sensing of biomarkers (e.g., DNA, RNA, proteins) using multiple electrode arrays (MEAs) has shown considerable promise.
Daggumati, Pallavi, Li, Zidong, Seker, Erkin, Veselinovic, Jovana   +3 more
core   +3 more sources