Glycerol Reduces Cross Hybridization on Nitrocellulose Membrane [PDF]
Lateral flow assay (LFD) based nucleic acid lateral flow (NALF) method has been developed recently. The method met point of care testing (POCT) as simple and rapid procedures, less equipment, and can be performance by less skilled technician. NALF based
Aman, Abu Tholib+3 more
core +2 more sources
Exploiting double exchange Diels-Alder cycloadditions for immobilization of peptide nucleic acids on gold nanoparticles [PDF]
The generation of PNA-decorated gold nanoparticles (AuNPs) has revealed to be more difficult as compared to the generation of DNA-functionalized ones. The less polar nature of this artificial nucleic acid system and the associated tendency of the neutral
Cadoni, Enrico+4 more
core +2 more sources
Nucleic acid analysis by sandwich hybridization [PDF]
Imperial Users ...
Nicholls, Peter John+1 more
core +1 more source
Detection of target DNA using fluorescent cationic polymer and peptide nucleic acid probes on solid support [PDF]
BACKGROUND: Nucleic acids detection using microarrays requires labelling of target nucleic acids with fluorophores or other reporter molecules prior to hybridization. RESULTS: Using surface-bound peptide nucleic acids (PNA) probes and soluble fluorescent
Bergeron, Michel G+7 more
core +3 more sources
Chemical Architecture and Applications of Nucleic Acid Derivatives Containing 1,2,3-Triazole Functionalities Synthesized via Click Chemistry [PDF]
There is considerable attention directed at chemically modifying nucleic acids with robust functional groups in order to alter their properties. Since the breakthrough of copper-assisted azide-alkyne cycloadditions (CuAAC), there have been several ...
Addepalli+40 more
core +1 more source
A method for nucleic acid hybridization to isolated chromosomes in suspension [PDF]
A procedure was developed to provide differential fluorescent staining of metaphase chromosomes in suspension following nucleic acid hybridization. For this purpose metaphase chromosomes were isolated from a Chinese hamster x human hybrid cell line ...
Bier, Frank+5 more
core +1 more source
Furan-PNA : a mildly inducible irreversible interstrand crosslinking system targeting single and double stranded DNA [PDF]
We here report on the design and synthesis of tailor-made furan-modified peptide nucleic acid (PNA) probes for covalent targeting of single stranded DNA through a crosslinking strategy.
Corradini, Roberto+3 more
core +2 more sources
Topological constraints in nucleic acid hybridization kinetics [PDF]
A theoretical examination of kinetic mechanisms for forming knots and links in nucleic acid structures suggests that molecules involving base pairs between loops are likely to become topologically trapped in persistent frustrated states through the ...
Bois, Justin S.+5 more
core +2 more sources
An ultrasensitive photoelectrochemical nucleic acid biosensor [PDF]
A simple and ultrasensitive procedure for non-labeling detection of nucleic acids is described in this study. It is based on the photoelectrochemical detection of target nucleic acids by forming a nucleic acid/photoreporter adduct layer on an ITO ...
Gao, Zhiqiang, Tansil, Natalia C.
core +3 more sources
Method for amplifying nucleic acids [PDF]
The present invention describes methods for amplifying a target nucleic acid wherein target nucleic acids hybridise to capture probe nucleic acids which are immobilized to a support via their 5 ' end.
Feitsma, Harma Martine+3 more
core