Results 91 to 100 of about 7,473,748 (355)

Delivery of DNA‐encoded vaccines and proteins in mice using cutaneous suction‐mediated transfection

FEBS Open Bio, EarlyView.
Cutaneous suction‐mediated transfection in mice for delivery of DNA‐encoded vaccines and proteins. A critical limitation of DNA vaccines and other therapeutics is transfection in vivo to produce the encoded antigens or therapeutic proteins. Cutaneous suction‐based methods have demonstrated effectiveness in many animal models and have been successfully ...
Emran O. Lallow, Isabel Brandtjen, Yaxin Mo, Melissa Gulley, Louis Osorio, Sagar Kudchodkar, Nandita C. Jhumur, Christine C. Roberts, Lisa K. Denzin, David I. Shreiber, Biju Parekkadan, Hao Lin, Joel N. Maslow   +12 more
wiley   +1 more source

Determinants of Genomic RNA Encapsidation in the Saccharomyces cerevisiae Long Terminal Repeat Retrotransposons Ty1 and Ty3

Viruses, 2016
Long-terminal repeat (LTR) retrotransposons are transposable genetic elements that replicate intracellularly, and can be considered progenitors of retroviruses.
Katarzyna Pachulska-Wieczorek, Stuart F.J. Le Grice, Katarzyna J. Purzycka   +2 more
doaj   +1 more source

Peptide nucleic acids: Advanced tools for biomedical applications

Journal of Biotechnology, 2017
Highlights • Peptide Nucleic Acids − DNA/RNA analogues.• Different Modifications on PNA backbone and their effects.• Neutral backbone − remarkable hybridization properties.• PNA based biosensors and their diverse biomedical applications.• Potential ...
Anjali Gupta, Anuradha Mishra, N. Puri
semanticscholar   +1 more source

Adenosine triphosphate as a modulator of protein interactions and stability

FEBS Open Bio, EarlyView.
ATP is best known as the cell's energy currency, but it also shapes how proteins fold, interact, aggregate and form biomolecular condensates. This review explains the emerging physical principles behind these effects, including weak binding to charged protein regions, magnesium‐dependent behaviour and concentration‐dependent control of protein ...
Shuyuan Tan, Robin Curtis
wiley   +1 more source

Interbase FRET in RNA: from A to Z [PDF]

, 2019
Published by Oxford University Press on behalf of Nucleic Acids Research. Interbase FRET can reveal highly detailed information about distance, orientation and dynamics in nucleic acids, complementing the existing structure and dynamics techniques.
Sandberg Wranne, Moa,, Wilhelmsson, L. M., Gr\uf6tli, Morten, Grötli, Morten,, Weis, Erik,, Nilsson, J. R., Dahlen, A., Weis, Erik, Wranne, M. S., Pfeiffer, Pauline, Bood, Mattias, Bood, Mattias,, Sandberg Wranne, Moa, Nilsson, Jesper, Grøtli, Mårten,, Fuchtbauer, A. F., Wilhelmsson, Marcus, Pfeiffer, P., Pfeiffer, Pauline,, F\ufcchtbauer, Anders Foller, Dahlén, Anders,, Nilsson, Jesper,, Füchtbauer, Anders Foller,, Dahl\ue9n, Anders, Wilhelmsson, Marcus,   +24 more
core   +1 more source

Phosphate steering by Flap Endonuclease 1 promotes 5′-flap specificity and incision to prevent genome instability

Nature Communications, 2017
Flap Endonuclease 1 is a DNA replication and repair enzyme indispensable for maintaining genomic stability. Here the authors provide mechanistic details on how FEN1 selects for 5′-flaps and promotes catalysis to avoid large-scale repeat expansion by a ...
Susan E. Tsutakawa, Mark J. Thompson, Andrew S. Arvai, Alexander J. Neil, Steven J. Shaw, Sana I. Algasaier, Jane C. Kim, L. David Finger, Emma Jardine, Victoria J.B. Gotham, Altaf H. Sarker, Mai Z. Her, Fahad Rashid, Samir M. Hamdan, Sergei M. Mirkin, Jane A. Grasby, John A. Tainer   +16 more
doaj   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Nucleic Acid Carriers Based on Precise Polymer Conjugates [PDF]

, 2011
Polymer polydispersity, random conjugation of functional groups, and poorly understood structure–activity relationships have constantly hampered progress in the development of nucleic acid carriers.
Christina Troiber, Troiber, Christina, Wagner, Ernst, Ernst Wagner   +3 more
core   +1 more source

Exploring RNA modifications in infectious non-coding circular RNAs

RNA Biology
Viroids, small circular non-coding RNAs, act as infectious pathogens in higher plants, demonstrating high stability despite consisting solely of naked RNA.
Pavel Vopalensky, Anton Škríba, Michela Chiumenti, Lucia Ďuričeková, Anna Šimonová, Ondřej Lukšan, Francesco Di Serio, Beatriz Navarro, Hana Cahova   +8 more
doaj   +1 more source

ConSurf 2010: calculating evolutionary conservation in sequence and structure of proteins and nucleic acids

Nucleic Acids Res., 2010
It is informative to detect highly conserved positions in proteins and nucleic acid sequence/structure since they are often indicative of structural and/or functional importance. ConSurf (http://consurf.tau.ac.il) and ConSeq (http://conseq.tau.ac.il) are
H. Ashkenazy, E. Erez, E. Martz, T. Pupko, N. Ben-Tal   +4 more
semanticscholar   +1 more source