Results 1 to 10 of about 7,439 (222)
RNase T1 mimicking artificial ribonuclease [PDF]
Nucleic Acids Research, 2007 Recently, artificial ribonucleases (aRNases)--conjugates of oligodeoxyribonucleotides and peptide (LR)(4)-G-amide--were designed and assessed in terms of the activity and specificity of RNA cleavage. The conjugates were shown to cleave RNA at Pyr-A and G-X sequences. Variations of oligonucleotide length and sequence, peptide and linker structure led to
N L Mironova +2 more
exaly +6 more sources
Enhanced RNA cleavage within bulge-loops by an artificial ribonuclease. [PDF]
Nucleic Acids Res, 2005 Cleavage of phosphodiester bonds by small ribonuclease mimics within different bulge-loops of RNA was investigated. Bulge-loops of different size (1-7 nt) and sequence composition were formed in a 3' terminal fragment of influenza virus M2 RNA (96 nt) by hybridization of complementary oligodeoxynucleotides. Small bulges (up to 4 nt) were readily formed
Kuznetsova IL +3 more
europepmc +4 more sources
Anticancer Activity of Reconstituted Ribonuclease S‐Decorated Artificial Viral Capsid
ChemBioChem, 2022 AbstractRibonuclease S (RNase S) is an enzyme that exhibits anticancer activity by degrading RNAs within cancer cells; however, the cellular uptake efficiency is low due to its small molecular size. Here we generated RNase S‐decorated artificial viral capsids with a size of 70–170 nm by self‐assembly of the β‐annulus‐S‐peptide followed by ...
Hiroto Furukawa +2 more
exaly +3 more sources
Organomercury oligonucleotide conjugates as artificial ribonucleases
Journal of Inorganic Biochemistry, 2023 Two oligonucleotide conjugates sharing the same sequence but incorporating a different 5'-terminal organometallic moiety were synthesized, by either direct mercuration in solution or oximation with an organomercury aldehyde on solid support. The potential of these conjugates to serve as new type of artificial ribonucleases was tested with a ...
Saleh Lange.Y. +2 more
openaire +2 more sources
Development of artificial ribonucleases [PDF]
Pure and Applied Chemistry, 1998 Abstract
Robert Häner +3 more
openaire +1 more source
Development of Site-specific Artificial Ribonucleases [PDF]
Nucleic Acids Symposium Series, 2007 Recently we found that an antisense 2'-O-methyloligonucleotide, with two terpyridine*Cu(II) complexes at contiguous internal sites, was highly active as a site-specific (sequence-specific) artificial ribonuclease, with the activity derived from the cooperative action of the complexes.
Hiroshi, Takayama +3 more
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Site-selective artificial ribonuclease using pinpoint RNA activation [PDF]
Nucleic Acids Symposium Series, 2001 An acridine residue attached to the end or the inside of a DNA activates the target phosphodiester linkages in complementary RNA. Due to this pinpoint activation, the RNA is site-selectively and efficiently cleaved at these linkages by various free metal ions under mild condition.
A, Kuzuya, R, Mizoguchi, M, Komiyama
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Design and Synthesis of Peptide-Oligonucleotide Conjugates as Potential Artificial Ribonucleases [PDF]
Nucleic Acids Symposium Series, 2008 A series of peptide-oligonucleotide conjugates was synthesized. The peptide fragment was attached to the middle part of DNA sequence. For this purpose the new amidophosphite dC with linker at C5-position was synthesized.
Inna, Serpokrylova +4 more
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CHIMIA, 2001 Chemically modified oligonucleotides serve as tools in fundamental research as diagnostic reagents and pharmaceutical compounds. Various chemical groups can nowadays be linked to oligonucleotides, In particular, synthetic constructs of oligonucleotides and lanthanide complexes can serve as artificial ribonucleases.
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Towards artificial ribonucleases: the sequence-specific cleavage of RNA in a duplex [PDF]
Nucleic Acids Research, 1996 Lanthanide complexes covalently attached to oligonucleotides have been shown to cleave RNA in a sequence-specific manner. Efficient cleavage, however, is at present limited to single-stranded RNA regions, as RNA in a duplex is considerably more resistant to strand scission.
J, Hall, D, Hüsken, R, Häner
openaire +2 more sources