Results 71 to 80 of about 105,644 (246)

cDNA Cloning of the Nucleocapsid and Nucleocapsid-associated Protein Genes of Mumps Virus

Journal of General Virology, 1985
cDNA clones of the mumps virus N and P messenger RNAs were isolated from an infected cell cDNA library. The N and P clones selected the two predominant polyadenylated RNAs found in mumps virus-infected cells with mol. wt. of 0.69 X 10(6) and 0.51 X 10(6), respectively. In addition, clones of the P gene hybridized to and selected mRNAs of higher mol. wt.
J A, Curran, J P, Quinn, E M, Hoey, S J, Martin, B K, Rima   +4 more
openaire   +2 more sources

Designing an ELISA Method for Measurement of Human IgG and IgM Antibodies against SARS-CoV-2

Journal of Mazandaran University of Medical Sciences, 2022
Background and purpose: Coronavirus disease 2019 (COVID-19) is a respiratory disease caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2).
Saeid Taghiloo, Mina Dabiri, Reza Valadan, Hossein Asgarian Omran   +3 more
doaj  

Differences in SARS‐CoV‐2 antigen persistence in individuals with systemic autoimmune rheumatic diseases compared to the general population: A RECOVER‐Adult Cohort Study

Arthritis &Rheumatology, Accepted Article.
Background Individuals with systemic autoimmune rheumatic diseases (SARDs) are at risk for worse acute and post‐acute COVID‐19 outcomes, though whether individuals with SARDs have longer persistence of viral antigens after COVID‐19 has not been studied.
Naomi J. Patel, Zoe Swank, Jiaqi Wang, Xiaosong Wang, Lauren A. O'Keeffe, Madison Negron, Liya S. Getachew, Louise L. Hansen, Grace Qian, Alene A. Saavedra, Kevin T. Mueller, Natalie A. Davis, Kathleen M.M. Vanni, Sandria Savage, Julie S. Lam, Zachary S. Wallace, David R. Walt, Jeffrey A. Sparks, RECOVER‐Adult   +18 more
wiley   +1 more source

Canine respiratory coronavirus employs caveolin-1-mediated pathway for internalization to HRT-18G cells [PDF]

, 2018
Canine respiratory coronavirus (CRCoV), identified in 2003, is a member of the Coronaviridae family. The virus is a betacoronavirus and a close relative of human coronavirus OC43 and bovine coronavirus.
A Milewska, A Pratelli, A Quan, Aleksandra Milewska, AM Zaki, Artur Szczepanski, B Hernaez, B Jancekova, BA Mainou, BJ Dille, C Burkard, C Kooi, C Liu, C Liu, D Dutta, DM Blau, E Dietzel, E Girard, E Hamme Van, EJ Bowman, F Samson, G Preta, GA Wudiri, GM Li, GR Ares, H Imhoff, H Lin, H Wang, IC Huang, IM Mackay, J Li, J Schindelin, J Somsel Rodman, JA Mitchell, JK Millet, JL Wang, JP Lim, JR Henley, Judy A. Mitchell, K Erles, K Erles, K Owczarek, K Shirato, K Shirato, Katarzyna Owczarek, Krzysztof Pyrc, KS Choi, L Kleist von, L Pelkmans, LH Wang, LJ Reed, M Marsh, MG Hemida, MJ Geisow, MJ Vincent, O Achour, O Heikkila, P Duckert, P Eifart, P Oh, Q Xu, R Nomura, S Bertram, S Bolte, S Gierer, S Matsuyama, SL Priestnall, TA Hill, VC Chu, X Ren, Y Inoue, Y Phonphok, Z Qian, Z Qinfen, Zbigniew Baster, Zenon Rajfur   +75 more
core   +8 more sources

Design of experiments‐guided membrane capture facilitates integration of continuous virus inactivation using Phi6 as a surrogate virus in an intensified downstream process

Biotechnology Progress, EarlyView.
Abstract The growing demand for cost‐efficient and flexible biomanufacturing has increased interest in process‐intensified downstream platforms. This study evaluates an intensified monoclonal antibody (mAb) purification sequence, where two unit operations traditionally performed in batch mode—Protein A capture and low pH virus inactivation (VI)—are ...
Mario Grünberg, Lisa Lipski, Gabriel Fisicaro, Thomas Duvignau, Karolina Meyer‐Heinrichs, Diana Carmen Mocsy, Thomas‐Josef Filz, Bastian Quaas, Alexandra Stützer   +8 more
wiley   +1 more source

The potential for reassortment between Oropouche and Schmallenberg Orthobunyaviruses [PDF]

, 2017
A number of viruses within the Peribunyaviridae family are naturally occurring reassortants, a common phenomenon for segmented viruses. Using a minigenome-reporter and virus-like particle (VLP) production assay, we have accessed the potential of ...
Elliott, Richard M., Olszanski Acrani, Gustavo, Shi, Xiaohong, Tilston-Lunel, Natasha L.   +3 more
core   +1 more source

Measles virus nucleocapsid protein expressed in insect cells assembles into nucleocapsid-like structures

Journal of General Virology, 1993
The gene encoding the major nucleocapsid, N, protein of measles virus has been inserted into a baculovirus vector under the control of the polyhedrin promoter. Insect cells infected with this recombinant baculovirus synthesize high levels of measles N protein, up to 40% of total soluble cell protein.
A R, Fooks, J R, Stephenson, A, Warnes, A B, Dowsett, B K, Rima, G W, Wilkinson   +5 more
openaire   +2 more sources

Preparation of Viral DNA from Nucleocapsids [PDF]

Journal of Visualized Experiments, 2011
Viruses are obligate cellular parasites, and thus the study of their DNA requires isolating viral material away from host cell contaminants and DNA. Several downstream applications require large quantities of pure viral DNA, which is provided by this protocol.
Moriah L, Szpara, Yolanda R, Tafuri, L W, Enquist   +2 more
openaire   +2 more sources

Modular characterization of SARS-CoV-2 nucleocapsid protein domain functions in nucleocapsid-like assembly

Molecular Biomedicine, 2023
AbstractSARS-CoV-2 and its variants, with the Omicron subvariant XBB currently prevailing the global infections, continue to pose threats on public health worldwide. This non-segmented positive-stranded RNA virus encodes the multi-functional nucleocapsid protein (N) that plays key roles in viral infection, replication, genome packaging and budding.
Yan Wang, Xiaobin Ling, Chong Zhang, Jian Zou, Bingnan Luo, Yongbo Luo, Xinyu Jia, Guowen Jia, Minghua Zhang, Junchao Hu, Ting Liu, Yuanfeiyi Wang, Kefeng Lu, Dan Li, Jinbiao Ma, Cong Liu, Zhaoming Su   +16 more
openaire   +3 more sources

Antigenic Properties of N Protein of Hantavirus

Viruses, 2014
Hantavirus causes two important rodent-borne viral zoonoses, hemorrhagic fever with renal syndrome (HFRS) in Eurasia and hantavirus pulmonary syndrome (HPS) in North and South America.
Kumiko Yoshimatsu, Jiro Arikawa
doaj   +1 more source