Results 81 to 90 of about 84,247 (263)

Endowing Flexibility of Alveolar Type II Cells to Recapitulate Development From Lung Bud to Alveoli

Advanced Science, EarlyView.
Adult human alveolar type 2 cells are chemically reprogrammed into expandable alveolar progenitor‐like cells that acquire bud tip‐like features during expansion and form organoids that mature into alveolar‐like structures. These organoids model influenza A virus infection and innate immune responses in vitro, while transplanted cells engraft ...
Hongqian Ma, Yuxin Zhang, Lujia Sun, Hong Jiang, Fan Li, Wenming Liu, Shenao Zhou, Mengting Wang, Weijian Huang, Xu Zhou, Caiyang Chen, Qiuqiu Zhang, Xiaoyan Jin, Feihan Yan, Hongping Wu, Yonghong Yin, Xinling Wang, Hexin Yan, Weifeng Yu   +18 more
wiley   +1 more source

Characterization of rabies virus nucleocapsids and recombinant nucleocapsid-like structures.

Journal of General Virology, 1998
Rabies virus nucleoprotein (N) was produced in insect cells using the baculovirus expression system described by Préhaud et al. (Virology 178, 486-497, 1990). The protein was either purified on a CsCl gradient, resulting in a mixture of nucleocapsid-like structures and beaded rings, as observed by electron microscopy, or on a glycerol gradient that ...
F, Iseni, A, Barge, F, Baudin, D, Blondel, R W, Ruigrok   +4 more
openaire   +2 more sources

Intrinsic disorder in measles virus nucleocapsids [PDF]

Proceedings of the National Academy of Sciences, 2011
The genome of measles virus is encapsidated by multiple copies of the nucleoprotein (N), forming helical nucleocapsids of molecular mass approaching 150 Megadalton. The intrinsically disordered C-terminal domain of N (N TAIL ) is essential for transcription and replication of the virus via ...
Malene Ringkjøbing, Jensen, Guillaume, Communie, Euripedes Almeida, Ribeiro, Nicolas, Martinez, Ambroise, Desfosses, Loïc, Salmon, Luca, Mollica, Frank, Gabel, Marc, Jamin, Sonia, Longhi, Rob W H, Ruigrok, Martin, Blackledge   +11 more
openaire   +2 more sources

Sarbecoviruses Suppress Innate Immunity by Recruiting PPM1A to Modulate STAT2 and ORF9b Phosphorylation

Advanced Science, EarlyView.
Sarbecovirus ORF9b recruits host phosphatase PPM1A to suppress innate immunity through dual mechanisms. PPM1A directly dephosphorylates ORF9b to sustain its activity and indirectly downregulates STAT2 phosphorylation to impair interferon signaling. Pharmacological inhibition of PPM1A restores antiviral responses and limits sarbecovirus replication ...
Lixiang Xie, Ziye Huang, Zhiyuan Zhang, Yiqiang Zhu, Xiaoqing Liu, Lei Wang, Tongyu Bi, Taizhen Liang, Jintao Lai, Meilin Hu, Guochang Qiu, Shiqi Xiao, Sen Liu, Yaoming Liu, Haiyue Rao, Tao Chen, Haojie Peng, Bin Zhang, Jia Tang, Qianying Li, Yaxin Li, Yuxin Hou, Weibo Yang, Hewei Jiang, Xiancai Ma   +24 more
wiley   +1 more source

Nucleocapsid- and Spike-specific IgG levels in saliva of newly positive, asymptomatic individuals versus prior positive, asymptomatic individuals.

, 2023
Saliva from NewPOS individuals infected with either CoV2US (n = 16) or CoV2Alpha (n = 15), as well as PCRNEG saliva from individuals who had been infected 2–37 weeks prior (PriorPOS, n = 402) with either CoV2US, CoV2Alpha, or a non-VOC, were used to ...
Jose A. Bazan (16859273), Abigail Norris Turner (7344479), Mikkel B. Quam (16859279), Jeff Jahnes (16859270), Alison Norris (2557534), Richard T. Robinson (12289326), Seth A. Faith (566702), Leona W. Ayers (9176825), Marisa Ruane-Foster (12289320), Najmus S. Mahfooz (16859267), Jacob Smith (1844773), Amanda Williams (102745), Marlena R. Merling (16859264), Michael Oglesbee (16859276)   +13 more
core   +1 more source

Antigenic Properties of N Protein of Hantavirus

Viruses, 2014
Hantavirus causes two important rodent-borne viral zoonoses, hemorrhagic fever with renal syndrome (HFRS) in Eurasia and hantavirus pulmonary syndrome (HPS) in North and South America.
Kumiko Yoshimatsu, Jiro Arikawa
doaj   +1 more source

Intracellular flow cytometry complements RT-qPCR detection of circulating SARS-CoV-2 variants of concern

BioTechniques, 2022
Basic and antiviral research on SARS-CoV-2 rely on cellular assays of virus replication in vitro. In addition, accurate detection of virus-infected cells and released virus particles is needed to study virus replication and to profile new candidate ...
Emiel Vanhulle, Becky Provinciael, Joren Stroobants, Anita Camps, Piet Maes, Kurt Vermeire   +5 more
doaj   +1 more source

An Oral Antiviral Combination Against Severe COVID‐19 Enabled by the Synergistic Mechanisms of Action of Auranofin and Remdesivir

Advanced Science, EarlyView.
FDA‐approved anti‐inflammatory drug Auranofin inhibits SARS‐CoV‐2 replication by inhibiting viral Main protease activity and Spike processing required for viral entry. It shows strong synergy with nucleoside analogue Remdesivir, due to non‐overlapping mechanisms.
Oyahida Khatun, Rohan Narayan, Santhosh Kambaiah Nagaraj, Rishad Shiraz, Abinaya Kaliappan, Sumandeep Kaur, Ankur Singh, Siva Shanmugam Selvaraj, Gudepalya Renukaiah Rudramurthy, Chakenahalli Naveenkumar, Shridhar Narayanan, Radha Krishan Shandil, Shailly Tomar, Shashank Tripathi   +13 more
wiley   +1 more source

Investigating the Heat Stability and Inactivation Conditions of Decapod Iridescent Virus 1 (DIV1)

Animal Research and One Health, EarlyView.
Heat treatment effectively inactivates decapod iridescent virus 1 (DIV1) under milder conditions than current WOAH recommendations. DIV1 infectivity was eliminated at 56°C for 30 min, 60°C for 15 min, or 70°C for 1 min, providing optimized heat treatment strategies for aquaculture biosecurity and seafood safety.
Yonghui Feng, Xiaomeng Guo, Jingyi Xing, Liang Qiu, Jie Huang   +4 more
wiley   +1 more source

Recent Advances in Virus–Host Interactions, Antiviral Bioactive Compounds, and Breeding for Disease Resistance of Porcine Epidemic Diarrhea Virus

Animal Research and One Health, EarlyView.
Basic research on the PEDV infection cycle and virus–host interactions advances the development of anti‐PEDV drugs and disease‐resistant breeding and helps strengthen disease prevention and control while reducing economic losses in the swine industry.
Heyong Wu, Aosi Qi, Shuyu Zhong, Haozhen Du, Shimeng Guo, Hongsheng Ouyang, Hongming Yuan, Daxin Pang, Zicong Xie   +8 more
wiley   +1 more source