Nucleoside deaminases: the key players in base editing toolkit. [PDF]
Biophys Rep, 2023 The development of nucleoside deaminase-containing base editors realized targeted single base change with high efficiency and precision. Such nucleoside deaminases include adenosine and cytidine deaminases, which can catalyze adenosine-to-inosine (A-to-I) and cytidine-to-uridine (C-to-U) conversion respectively.
Xiang J +5 more
europepmc +3 more sources
Nucleoside Analogs in ADAR Guide Strands Enable Editing at 5′-GA Sites [PDF]
BiomoleculesAdenosine Deaminases Acting on RNA (ADARs) are members of a family of RNA editing enzymes that catalyze the conversion of adenosine into inosine in double-stranded RNA (dsRNA).
Aashrita Manjunath +9 more
doaj +2 more sources
Synthesis of 1,4-azaphosphinine nucleosides and evaluation as inhibitors of human cytidine deaminase and APOBEC3A [PDF]
Beilstein Journal of Organic ChemistryNucleoside and polynucleotide cytidine deaminases (CDAs), such as CDA and APOBEC3, share a similar mechanism of cytosine to uracil conversion. In 1984, phosphapyrimidine riboside was characterised as the most potent inhibitor of human CDA, but the quick ...
Maksim V. Kvach +5 more
doaj +2 more sources
Efficient Biocatalytic Synthesis of Dihalogenated Purine Nucleoside Analogues Applying Thermodynamic Calculations [PDF]
, 2020 The enzymatic synthesis of nucleoside analogues has been shown to be a sustainable and efficient alternative to chemical synthesis routes. In this study, dihalogenated nucleoside analogues were produced by thermostable nucleoside phosphorylases in ...
Giessmann, Robert T. +9 more
core +1 more source
DNA editing in DNA/RNA hybrids by adenosine deaminases that act on RNA. [PDF]
, 2017 Adenosine deaminases that act on RNA (ADARs) carry out adenosine (A) to inosine (I) editing reactions with a known requirement for duplex RNA. Here, we show that ADARs also react with DNA/RNA hybrid duplexes.
Beal, Peter A +2 more
core +1 more source
Trading in cooperativity for specificity to maintain uracil-free DNA [PDF]
, 2015 Members of the dUTPase superfamily play an important role in the maintenance of the pyrimidine nucleotide balance and of genome integrity. dCTP deaminases and the bifunctional dCTP deaminase-dUTPases are cooperatively regulated by dTTP.
Merényi, Gábor +4 more
core +3 more sources
Expanding a fluorescent RNA alphabet: synthesis, photophysics and utility of isothiazole-derived purine nucleoside surrogates. [PDF]
, 2017 A series of emissive ribonucleoside purine mimics, all comprised of an isothiazolo[4,3-d]pyrimidine core, was prepared using a divergent pathway involving a key Thorpe-Ziegler cyclization.
Fin, Andrea +2 more
core +1 more source
mSphere, 2019 Cytidine deaminase (CDA) is a pyrimidine salvage enzyme that catalyzes cytidine and deoxycytidine hydrolytic deamination to yield uridine and deoxyuridine.
Ana Moro-Bulnes +6 more
doaj +1 more source
Activation-induced deoxycytidine deaminase (AID) co-transcriptional scanning at single-molecule resolution [PDF]
, 2015 Activation-induced deoxycytidine deaminase (AID) generates antibody diversity in B cells by initiating somatic hypermutation (SHM) and class-switch recombination (CSR) during transcription of immunoglobulin variable (IgV) and switch region (IgS) DNA ...
Bertram, JG +7 more
core +1 more source
DNA replication stress mediates APOBEC3 family mutagenesis in breast cancer [PDF]
, 2016 BACKGROUND: The APOBEC3 family of cytidine deaminases mutate the cancer genome in a range of cancer types. Although many studies have documented the downstream effects of APOBEC3 activity through next-generation sequencing, less is known about their ...
Bartek, J +16 more
core +8 more sources