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Synthesis of Nonnatural Nucleoside Diphosphate Sugars

European Journal of Organic Chemistry, 2011
AbstractRecently, we reported an efficient chemical method for the synthesis of a variety of naturally occurring nucleoside diphosphate (NDP) sugars. This method, which is based on the cycloSal approach, can also be used, in principle, for the preparation of rare or even nonnatural NDP sugars. Herein, the syntheses of sulfoquinovose‐, glucose‐6‐sulfate‐
Saskia Wolf   +2 more
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The metal ion catalyzed decomposition of nucleoside diphosphate sugars

Biochemistry, 1976
The metal ion catalysed decomposition of the nucleotide diphosphate sugars, uridine diphosphate glucose, uriding diphosphate galactose, uridine diphosphate N-acetylglucosamine, guanosine diphosphate mannose, and guanosine diphosphate fucose (UDPGlc, UDPGal, UDPGlc-NAc, GDPMan, and GDPFuc, respectively), has been studies as a function of pH.
H A, Nunez, R, Barker
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A Simple Chemical Synthesis of Sugar Nucleoside Diphosphates in Water

Current Protocols in Nucleic Acid Chemistry, 2013
AbstractChemoenzymatic oligosaccharide synthesis is attractive since it eliminates the tedious multistep protection‐deprotection requirements of pure chemical synthesis. Chemoenzymatic synthesis using glycosyltransferases, however, requires not only the correct enzyme to control both regio‐ and stereospecificity, but also the glycosyl donor to provide ...
Hidenori, Tanaka   +2 more
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A spectrophotometric method for the determination of nucleoside triphosphates (pyrophosphorolysis of nucleoside diphosphate sugars)

Analytical Biochemistry, 1965
Abstract The enzyme-catalyzed pyrophosphorolysis of nucleoside diphosphate sugars has been followed spectrophotometrically using 3-P-glycerate kinase (EC 2.7.2.3) and glyceraldehyde-3-P dehydrogenase (EC 1.2.1.12) coupled to the oxidation of NADH. The method is rapid, and gives a direct recording of enzyme activity. Some limitations are discussed but
H, Verachtert   +3 more
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[34] Sugar nucleotide phosphorylases (“nucleoside diphosphate sugar: Orthophosphate nucleotidyl transferases”)

1966
Publisher Summary This chapter discusses the synthesis of sugar nucleotide phosphorylases from yeast and from wheat germ. In the assay method for yeast, the reaction is carried out using guanosine diphosphate (GDP) mannose and 32 P. The radioactive GDP produced during incubation is separated from 32 P i by adsorption on charcoal.
Enrico Cabib, Hector Carminatti
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Synthetic analogues of uridine diphosphate glucose: Biochemical and chemical studies The secondary stucture of nucleoside diphosphate sugars

Biochimica et Biophysica Acta (BBA) - Enzymology and Biological Oxidation, 1966
Abstract The reactivity of eight synthetic analogues of uridine diphosphate glucose with a modified uracyl nucleus in four enzymic systems of uridine diphosphate glucose metabolism has been investigated. For the activity of all of the enzymes studied, the presence of an acylamido grouping −C2(X)N3HC4(X)− (where X = O or S ), appeared necessary.
E I, Budowsky   +7 more
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