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A Simple Synthesis of Sugar Nucleoside Diphosphates by Chemical Coupling in Water
Angewandte Chemie International Edition, 2012The use of glycosyltransferases in chemoenzymatic oligosaccharide synthesis is attractive, provided that the enzymes are available, since it eliminates the tedious multistep protectiondeprotection and chemical glycosylation procedure that characterizes classic chemical synthesis.
Hidenori, Tanaka +4 more
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An improved method for the isolation of some nucleoside diphosphate sugars from yeast
Biochimica et Biophysica Acta, 1957Abstract An improved method is described for the simultaneous isolation of UDPG, UDPAG and GDPM from yeast. The method involves extraction with ethanol and chromatography on anion exchange resin columns. The nucleotides are obtained from the effluent by adsorption on charcoal and subsequent elution with aqueous ethanol. A washing of the charcoal with
H G, PONTIS, E, CABIB, L F, LELOIR
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ChemBioChem, 2002
The reaction mechanism of the phosphoryl transfer catalyzed by dinucleoside diphosphate kinase from Dictyostelium discoideum is investigated by semiempirical AM1 molecular orbital computation of an active site model system on the basis of various X-ray crystallographic structures.
Michael C, Hutter, Volkhard, Helms
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The reaction mechanism of the phosphoryl transfer catalyzed by dinucleoside diphosphate kinase from Dictyostelium discoideum is investigated by semiempirical AM1 molecular orbital computation of an active site model system on the basis of various X-ray crystallographic structures.
Michael C, Hutter, Volkhard, Helms
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, 1979 1966
Publisher Summary This chapter discusses the synthesis of sugar nucleotide phosphorylases from yeast and from wheat germ. In the assay method for yeast, the reaction is carried out using guanosine diphosphate (GDP) mannose and 32 P. The radioactive GDP produced during incubation is separated from 32 P i by adsorption on charcoal.
Enrico Cabib, Hector Carminatti
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Publisher Summary This chapter discusses the synthesis of sugar nucleotide phosphorylases from yeast and from wheat germ. In the assay method for yeast, the reaction is carried out using guanosine diphosphate (GDP) mannose and 32 P. The radioactive GDP produced during incubation is separated from 32 P i by adsorption on charcoal.
Enrico Cabib, Hector Carminatti
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Organic Letters
Pyrophosphates are central to biology, and synthetic pyrophosphate isosteres are widely sought to create biomimetic molecules to study diverse biochemical processes. We describe a flexible, modular synthesis of nucleoside diphosphate sugar analogues by linking carbasugars and nucleosides with the dual electrophilic reagents chlorosulfonyl isocyanate ...
Steneker, R. +8 more
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Pyrophosphates are central to biology, and synthetic pyrophosphate isosteres are widely sought to create biomimetic molecules to study diverse biochemical processes. We describe a flexible, modular synthesis of nucleoside diphosphate sugar analogues by linking carbasugars and nucleosides with the dual electrophilic reagents chlorosulfonyl isocyanate ...
Steneker, R. +8 more
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Chemischer Informationsdienst, 1983
AbstractEs wird eine einfache Methode zur Umwandlung von Hefe‐RNA in ein Gemisch aus ATP, UTP, GTP und CTP beschrieben.
C.‐H. WONG +2 more
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AbstractEs wird eine einfache Methode zur Umwandlung von Hefe‐RNA in ein Gemisch aus ATP, UTP, GTP und CTP beschrieben.
C.‐H. WONG +2 more
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Periplasmic nucleoside diphosphate sugar hydrolase from Bacillus subtilis
Biochemistry, 1970J, Mauck, L, Glaser
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[THE STRUCTURE AND FUNCTION OF NUCLEOSIDE DIPHOSPHATE SUGARS].
Biokhimiia (Moscow, Russia), 1996N K, KOCHETKOV +2 more
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