Results 121 to 130 of about 6,705 (157)
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Biochimica et Biophysica Acta (BBA) - Enzymology and Biological Oxidation, 1966
Abstract The reactivity of eight synthetic analogues of uridine diphosphate glucose with a modified uracyl nucleus in four enzymic systems of uridine diphosphate glucose metabolism has been investigated. For the activity of all of the enzymes studied, the presence of an acylamido grouping −C2(X)N3HC4(X)− (where X = O or S ), appeared necessary.
E I, Budowsky +7 more
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Abstract The reactivity of eight synthetic analogues of uridine diphosphate glucose with a modified uracyl nucleus in four enzymic systems of uridine diphosphate glucose metabolism has been investigated. For the activity of all of the enzymes studied, the presence of an acylamido grouping −C2(X)N3HC4(X)− (where X = O or S ), appeared necessary.
E I, Budowsky +7 more
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Journal of Medicinal Chemistry, 1987
The synthesis of alpha-D-glucopyranosyl-, alpha-D-galactopyranosyl-, and alpha-D-mannopyranosylphosphonate is described. Condensation of tris(trimethylsilyl) phosphite with 2,3,4,6-tetrakis-O-(phenylmethyl)-1-O-acetyl-alpha-D-glucopyranose generated 2,3,4,6-tetrakis-O-(phenylmethyl)-alpha-D-glucopyranosylphosphonic acetic anhydride (13).
M M, Vaghefi +4 more
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The synthesis of alpha-D-glucopyranosyl-, alpha-D-galactopyranosyl-, and alpha-D-mannopyranosylphosphonate is described. Condensation of tris(trimethylsilyl) phosphite with 2,3,4,6-tetrakis-O-(phenylmethyl)-1-O-acetyl-alpha-D-glucopyranose generated 2,3,4,6-tetrakis-O-(phenylmethyl)-alpha-D-glucopyranosylphosphonic acetic anhydride (13).
M M, Vaghefi +4 more
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An improved P(V)-N activation strategy for the synthesis of nucleoside diphosphate 6-deoxy-l-sugars
Tetrahedron, 2014Abstract Four nucleoside diphosphate 6-deoxy- l -sugars have been efficiently synthesized by coupling sugar-1-phosphates prepared from the H-phosphonate precursors with nucleoside 5′-phosphoropiperidates in the presence of 4,5-dicyanoimidazole (DCI) as the activator. Compared to the conventional 1H-tetrazole-promoted phosphoromorpholidate method, the
Qi Sun +5 more
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Chemical and enzymic degradations of nucleoside mono- and diphosphate sugars
Biochimica et Biophysica Acta (BBA) - General Subjects, 1979G. Spik, P. Six, J. Montreuil
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A Simple Chemical Synthesis of Sugar Nucleoside Diphosphates in Water
Current Protocols in Nucleic Acid Chemistry, 2013AbstractChemoenzymatic oligosaccharide synthesis is attractive since it eliminates the tedious multistep protection‐deprotection requirements of pure chemical synthesis. Chemoenzymatic synthesis using glycosyltransferases, however, requires not only the correct enzyme to control both regio‐ and stereospecificity, but also the glycosyl donor to provide ...
Hidenori, Tanaka +2 more
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Analytical Biochemistry, 1965
Abstract The enzyme-catalyzed pyrophosphorolysis of nucleoside diphosphate sugars has been followed spectrophotometrically using 3-P-glycerate kinase (EC 2.7.2.3) and glyceraldehyde-3-P dehydrogenase (EC 1.2.1.12) coupled to the oxidation of NADH. The method is rapid, and gives a direct recording of enzyme activity. Some limitations are discussed but
H, Verachtert +3 more
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Abstract The enzyme-catalyzed pyrophosphorolysis of nucleoside diphosphate sugars has been followed spectrophotometrically using 3-P-glycerate kinase (EC 2.7.2.3) and glyceraldehyde-3-P dehydrogenase (EC 1.2.1.12) coupled to the oxidation of NADH. The method is rapid, and gives a direct recording of enzyme activity. Some limitations are discussed but
H, Verachtert +3 more
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1966
Publisher Summary This chapter discusses the synthesis of sugar nucleotide phosphorylases from yeast and from wheat germ. In the assay method for yeast, the reaction is carried out using guanosine diphosphate (GDP) mannose and 32 P. The radioactive GDP produced during incubation is separated from 32 P i by adsorption on charcoal.
Enrico Cabib, Hector Carminatti
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Publisher Summary This chapter discusses the synthesis of sugar nucleotide phosphorylases from yeast and from wheat germ. In the assay method for yeast, the reaction is carried out using guanosine diphosphate (GDP) mannose and 32 P. The radioactive GDP produced during incubation is separated from 32 P i by adsorption on charcoal.
Enrico Cabib, Hector Carminatti
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Regulation of the Synthesis of Nucleoside Diphosphate Sugars in Reticulo-Endothelial Tissues
European Journal of Biochemistry, 1975The kinetic and regulatory properties of enzymes involved in the biosynthesis of UDP-D-galactose, UDP-N-acetylglucosamine. GDP-alpha-D-mannose and GDP-beta-L-fucose from D-glucose 6-phosphate in various reticulo-endothelial tissues was studied. The tissues examined include bovine liver, thyroid, spleen, salivary gland, lung, intestine and mesenteric ...
J, Mendicino, A K, Rao
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A Simple Synthesis of Sugar Nucleoside Diphosphates by Chemical Coupling in Water
Angewandte Chemie International Edition, 2012The use of glycosyltransferases in chemoenzymatic oligosaccharide synthesis is attractive, provided that the enzymes are available, since it eliminates the tedious multistep protectiondeprotection and chemical glycosylation procedure that characterizes classic chemical synthesis.
Hidenori, Tanaka +4 more
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