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An improved method for the isolation of some nucleoside diphosphate sugars from yeast
Biochimica et Biophysica Acta, 1957Abstract An improved method is described for the simultaneous isolation of UDPG, UDPAG and GDPM from yeast. The method involves extraction with ethanol and chromatography on anion exchange resin columns. The nucleotides are obtained from the effluent by adsorption on charcoal and subsequent elution with aqueous ethanol. A washing of the charcoal with
H G, PONTIS, E, CABIB, L F, LELOIR
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ChemBioChem, 2002
The reaction mechanism of the phosphoryl transfer catalyzed by dinucleoside diphosphate kinase from Dictyostelium discoideum is investigated by semiempirical AM1 molecular orbital computation of an active site model system on the basis of various X-ray crystallographic structures.
Michael C, Hutter, Volkhard, Helms
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The reaction mechanism of the phosphoryl transfer catalyzed by dinucleoside diphosphate kinase from Dictyostelium discoideum is investigated by semiempirical AM1 molecular orbital computation of an active site model system on the basis of various X-ray crystallographic structures.
Michael C, Hutter, Volkhard, Helms
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1966
Publisher Summary This chapter discusses the synthesis of sugar nucleotide phosphorylases from yeast and from wheat germ. In the assay method for yeast, the reaction is carried out using guanosine diphosphate (GDP) mannose and 32 P. The radioactive GDP produced during incubation is separated from 32 P i by adsorption on charcoal.
Enrico Cabib, Hector Carminatti
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Publisher Summary This chapter discusses the synthesis of sugar nucleotide phosphorylases from yeast and from wheat germ. In the assay method for yeast, the reaction is carried out using guanosine diphosphate (GDP) mannose and 32 P. The radioactive GDP produced during incubation is separated from 32 P i by adsorption on charcoal.
Enrico Cabib, Hector Carminatti
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Organic Letters
Pyrophosphates are central to biology, and synthetic pyrophosphate isosteres are widely sought to create biomimetic molecules to study diverse biochemical processes. We describe a flexible, modular synthesis of nucleoside diphosphate sugar analogues by linking carbasugars and nucleosides with the dual electrophilic reagents chlorosulfonyl isocyanate ...
Steneker, R. +8 more
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Pyrophosphates are central to biology, and synthetic pyrophosphate isosteres are widely sought to create biomimetic molecules to study diverse biochemical processes. We describe a flexible, modular synthesis of nucleoside diphosphate sugar analogues by linking carbasugars and nucleosides with the dual electrophilic reagents chlorosulfonyl isocyanate ...
Steneker, R. +8 more
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Periplasmic nucleoside diphosphate sugar hydrolase from Bacillus subtilis
Biochemistry, 1970J, Mauck, L, Glaser
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[136] Periplasmic enzymes that hydrolyze nucleoside diphosphate sugars
1972Luis Glaser, John Mauck
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[THE STRUCTURE AND FUNCTION OF NUCLEOSIDE DIPHOSPHATE SUGARS].
Biokhimiia (Moscow, Russia), 1996N K, KOCHETKOV +2 more
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An improved P(V)-N activation strategy for the synthesis of nucleoside diphosphate 6-deoxy-l-sugars
Tetrahedron, 2014exaly
S1.8 The use of nucleoside diphosphate sugar photoaffinity probes to isolate glycosyltransferases
Glycoconjugate Journal, 1993A. D. Elbein +3 more
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