Results 131 to 140 of about 3,075 (166)
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Analytical Biochemistry, 1965
Abstract The enzyme-catalyzed pyrophosphorolysis of nucleoside diphosphate sugars has been followed spectrophotometrically using 3-P-glycerate kinase (EC 2.7.2.3) and glyceraldehyde-3-P dehydrogenase (EC 1.2.1.12) coupled to the oxidation of NADH. The method is rapid, and gives a direct recording of enzyme activity. Some limitations are discussed but
S T Bass, R G Hansen
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Abstract The enzyme-catalyzed pyrophosphorolysis of nucleoside diphosphate sugars has been followed spectrophotometrically using 3-P-glycerate kinase (EC 2.7.2.3) and glyceraldehyde-3-P dehydrogenase (EC 1.2.1.12) coupled to the oxidation of NADH. The method is rapid, and gives a direct recording of enzyme activity. Some limitations are discussed but
S T Bass, R G Hansen
exaly +3 more sources
Ion-exchange paper chromatography of nucleoside diphosphate sugars and related nucleotides
Analytical Biochemistry, 1965Abstract A new method especially adapted for the characterization of nucleoside diphosphate sugars in mixtures containing nucleoside mono-, di-, and tri-phosphates is described. It is based on ion-exchange chromatography using papers impregnated with polyethyleneimine (PEI).
Hubert Verachtert +2 more
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Biochimica Et Biophysica Acta: BBA Enzymology and Biological Oxidation, 1966
Abstract The reactivity of eight synthetic analogues of uridine diphosphate glucose with a modified uracyl nucleus in four enzymic systems of uridine diphosphate glucose metabolism has been investigated. For the activity of all of the enzymes studied, the presence of an acylamido grouping −C2(X)N3HC4(X)− (where X = O or S ), appeared necessary.
E I Budowsky +2 more
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Abstract The reactivity of eight synthetic analogues of uridine diphosphate glucose with a modified uracyl nucleus in four enzymic systems of uridine diphosphate glucose metabolism has been investigated. For the activity of all of the enzymes studied, the presence of an acylamido grouping −C2(X)N3HC4(X)− (where X = O or S ), appeared necessary.
E I Budowsky +2 more
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Journal of Medicinal Chemistry, 1987
The synthesis of alpha-D-glucopyranosyl-, alpha-D-galactopyranosyl-, and alpha-D-mannopyranosylphosphonate is described. Condensation of tris(trimethylsilyl) phosphite with 2,3,4,6-tetrakis-O-(phenylmethyl)-1-O-acetyl-alpha-D-glucopyranose generated 2,3,4,6-tetrakis-O-(phenylmethyl)-alpha-D-glucopyranosylphosphonic acetic anhydride (13).
Ralph J Bernacki +2 more
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The synthesis of alpha-D-glucopyranosyl-, alpha-D-galactopyranosyl-, and alpha-D-mannopyranosylphosphonate is described. Condensation of tris(trimethylsilyl) phosphite with 2,3,4,6-tetrakis-O-(phenylmethyl)-1-O-acetyl-alpha-D-glucopyranose generated 2,3,4,6-tetrakis-O-(phenylmethyl)-alpha-D-glucopyranosylphosphonic acetic anhydride (13).
Ralph J Bernacki +2 more
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Chemical and enzymic degradations of nucleoside mono- and diphosphate sugars
Biochimica Et Biophysica Acta - General Subjects, 1979G Spik, Jean Montreuil
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[136] Periplasmic enzymes that hydrolyze nucleoside diphosphate sugars
, 1972 Luis Glaser, John Mauck
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THE ROLE OF PYRIDINE NUCLEOTIDES IN INTERCONVERSIONS OF NUCLEOSIDE DIPHOSPHATE SUGARS
, 1972 David Sidney Feingold
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Journal of the American Chemical Society, 1983
Chi Huey Wong, George M Whitesides
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Chi Huey Wong, George M Whitesides
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A Simple Chemical Synthesis of Sugar Nucleoside Diphosphates in Water
Current Protocols in Nucleic Acid Chemistry, 2013AbstractChemoenzymatic oligosaccharide synthesis is attractive since it eliminates the tedious multistep protection‐deprotection requirements of pure chemical synthesis. Chemoenzymatic synthesis using glycosyltransferases, however, requires not only the correct enzyme to control both regio‐ and stereospecificity, but also the glycosyl donor to provide ...
Hidenori, Tanaka +2 more
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Regulation of the Synthesis of Nucleoside Diphosphate Sugars in Reticulo-Endothelial Tissues
European Journal of Biochemistry, 1975The kinetic and regulatory properties of enzymes involved in the biosynthesis of UDP-D-galactose, UDP-N-acetylglucosamine. GDP-alpha-D-mannose and GDP-beta-L-fucose from D-glucose 6-phosphate in various reticulo-endothelial tissues was studied. The tissues examined include bovine liver, thyroid, spleen, salivary gland, lung, intestine and mesenteric ...
J, Mendicino, A K, Rao
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