Results 81 to 90 of about 151,614 (341)

Steric‐Adaptive Biocatalysis: Imine Reductase‐Mediated Dynamic Kinetic Resolution for Atroposelective Synthesis of Hindered Biaryl Amines

Advanced Science, EarlyView.
An unusual IRED platform is reported, enabling efficient DKR via reductive amination without any evolutionary engineering. The system accommodates extremely sterically demanding substrates, including naphthyl and quinoline derivatives, delivering naphthyl‐aryl and quinoline‐aryl amine atropisomers with excellent efficiency and stereocontrol (over 80 ...
Zhichao Ni, Jingyuan Zhuang, Yadong Gao, Guangsheng Gao, Zhenling Liu, Ping Su, Gui‐Juan Cheng, Li‐Cheng Yang   +7 more
wiley   +1 more source

Immobilization of Neutral Protease from Bacillus subtilis for Regioselective Hydrolysis of Acetylated Nucleosides: Application to Capecitabine Synthesis

Molecules, 2016
This paper describes the immobilization of the neutral protease from Bacillus subtilis and its application in the regioselective hydrolysis of acetylated nucleosides, including building blocks useful for the preparation of anticancer products.
Teodora Bavaro, Giulia Cattaneo, Immacolata Serra, Ilaria Benucci, Massimo Pregnolato, Marco Terreni   +5 more
doaj   +1 more source

Dynamic eIF3‐S6 Phase Separation Switch Instructed by m6A Modification Drives the Molting of Locusts

Advanced Science, EarlyView.
This research reveals that reduced m6A modifications during insect molting trigger liquid–liquid phase separation (LLPS) of the m6A reader eIF3‐S6, which binds and stabilizes key molting mRNAs, including EcR and Cht10. Disrupting m6A or LLPS causes cuticle remodeling defects and molting failure, highlighting the synergistic control of gene expression ...
Zhihao Hu, Yu Zhou, Huimin Wang, Yaxi Wu, Rui Han, Shili Liu, Feng Jiang, Xiaojiao Guo, Meiling Yang   +8 more
wiley   +1 more source

Synthesis, Biological Activity, and Molecular Dynamics Simulations of LNA‐Charge Neutral Linkages for Enhanced Splice‐Switching Antisense Oligonucleotides

Angewandte Chemie, EarlyView.
Splice‐switching oligonucleotides are used to treat severe genetic conditions including Duchenne Muscular Dystrophy, but new chemistries are urgently required to improve their efficacy. In this work locked nucleic acid (LNA) is coupled to different charge neutral DNA backbones which are studied by structural, thermodynamic, and biological methods.
Alice Kennett, Lillian Lie, Martin Flerin, Belma Zengin Kurt, Ysobel R. Baker, Alyssa C. Hill, Abinaya Ramesh, Matthew J.A. Wood, Debashis Dhara, Afaf H. El‐Sagheer, Fernanda Duarte, Tom Brown   +11 more
wiley   +2 more sources

Nucleoside Triphosphate-Nucleoside Diphosphate Transphosphorylase (Nucleoside Diphosphokinase)

Journal of Biological Chemistry, 1964
Abstract Previous physical studies from this laboratory by sedimentation velocity and diffusion and by sedimentation equilibrium (Yue, R. H., Ratliff, R. L., and Kuby, S. A. (1967) Biochemistry 6, 2923–2932) have permitted an assignment of the kinetic molecular weight of the crystalline brewers' yeast nucleoside diphosphokinase, namely 102,000 (±2,000)
Lily Wu, Richard H. Palmieri, Hans K. Jacobs, Lynn Maland, Stephen A. Kuby, Robert H. Yue   +5 more
openaire   +2 more sources

Enzalutamide‐Resistant STEAP4+ MyoCAF Secrete Phosphatidylcholine to Foster Progression by Activating Stemness in Hormone‐Sensitive Prostate Cancer

Advanced Science, EarlyView.
Expanding enzalutamide (ENZ) use in hormone‐sensitive prostate cancer (HSPC) faces resistance. Single‐cell profiling identifies therapy‐resistant STEAP4+ myofibroblastic cancer‐associated fibroblast (SETAP4+ myoCAF). These myoCAFs drive resistance via TFE3‐mediated autophagy and PCYT1A‐led phosphatidylcholine overproduction, creating a phospholipid ...
Wenhao Wang, Jing Zhao, Tiewen Li, Guangjian Fan, Jianong Zhang, Chenghao Zheng, Zhiwen Xie, Yu Zhang, Chengling Feng, Tianyu Cao, Jianyin Li, Guomin Ju, Di Cui, Shujie Xia, Bangmin Han   +14 more
wiley   +1 more source

Sequence‐Specific Installation of Aryl Groups in RNA via DNA‐Catalyst Conjugates

Angewandte Chemie, EarlyView.
DNA oligonucleotides carrying amine nucleophilic catalysts are employed to deliver aryl groups to complementary sites in RNA under physiological conditions. The strategy can be used to sequence‐specifically label protein‐coding RNAs, or to knock down expression selectively, with potential applications in basic transcriptome research and in future ...
Sumon Pratihar, Wenrui Zhong, Sheng Feng, Sayantan Chatterjee, Eric T. Kool   +4 more
wiley   +2 more sources

Distinct Phage‐Encoded Enzymes for Substitution of Deoxythymidine by Deoxyuridine in Phage Genomes

Advanced Science, EarlyView.
Distinct enzymes including dCTP deaminases (Dcds), dTMP phosphatases (Dtms), and dTTP pyrophosphatases (Dtt) are found to be responsible for dU‐DNA biosynthesis in the phages PBS1, DSS3_VP1, and PhiR1‐37. Both PhiR1‐37‐derived and synthetic dU‐DNA are resistant to cleavage by restriction enzymes and Cas12a nuclease targeting dT‐containing sites.
Yating Li, Jason Tan, Yanqin Tu, Jingnan Wu, Zaifang Zhang, Yifeng Wei, Xinan Jiao, Yan Zhou   +7 more
wiley   +1 more source

Synthesis, reactivity and biological activity of 5-alkoxymethyluracil analogues

Beilstein Journal of Organic Chemistry, 2011
This review article summarizes the results of a long-term investigation of 5-alkoxymethyluracil analogues and is aimed, in particular, at methods of syntheses.
Lucie Brulikova, Jan Hlavac
doaj   +1 more source

Ion-Exchange Chromatography of Nucleoside Polyphosphates. [PDF]

, 1954
Rolf Bergkvist, Adam Deutsch, Chr. Klixbüll Jørgensen, Nils Andreas Sörensen   +3 more
openalex   +1 more source