Synthesis and Antiviral Activity of a Series of 2′-C-Methyl-4′-thionucleoside Monophosphate Prodrugs
The NS5B RNA-dependent RNA polymerase of the hepatitis C virus (HCV) is a validated target for nucleoside antiviral drug therapy. We endeavored to synthesize and test a series of 4′-thionucleosides with a monophosphate prodrug moiety for their antiviral ...
Zackery W. Dentmon+2 more
doaj +1 more source
Determination of total potentially available nucleosides in bovine milk [PDF]
Bovine colostrum and milk samples were collected from two herds over the course of the first month post-partum, pooled for each herd by stage of lactation and total potentially available nucleosides were determined.
Gill, Brendon D.+2 more
core +2 more sources
Thermophilic phosphoribosyltransferases Thermus thermophilus HB27 in nucleotide synthesis
Phosphoribosyltransferases are the tools that allow the synthesis of nucleotide analogues using multi-enzymatic cascades. The recombinant adenine phosphoribosyltransferase (TthAPRT) and hypoxanthine phosphoribosyltransferase (TthHPRT) from Thermus ...
Ilja V. Fateev+9 more
doaj +1 more source
Statistical distributions of sequencing by synthesis with probabilistic nucleotide incorporation [PDF]
Sequencing by synthesis is used in many next-generation DNA sequencing technologies. Some of the technologies, especially those exploring the principle of single-molecule sequencing, allow incomplete nucleotide incorporation in each cycle. We derive statistical distributions for sequencing by synthesis by taking into account the possibility that ...
arxiv +1 more source
Enzyme-free genetic copying of DNA and RNA sequences
The copying of short DNA or RNA sequences in the absence of enzymes is a fascinating reaction that has been studied in the context of prebiotic chemistry.
Marilyne Sosson, Clemens Richert
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Binding Group of Oligonucleotides on TiO 2 Surfaces: Phosphate Anions or Nucleobases? [PDF]
Although the immobilization of oligonucleotides (nucleic acid) on mineral surfaces is at the basis of different biotechnological applications, an atomistic understanding of the interaction of the nucleic acid components with the titanium dioxide surfaces has not yet been achieved.
arxiv +1 more source
Characterisation of multiple substrate-specific (d)ITP/(d)XTPase and modelling of deaminated purine nucleotide metabolism [PDF]
To be viable, organisms possess a number of (deoxy)nucleotide phosphohydrolases, which hydrolyze these nucleotides removing them from the active NTP and dNTP pools. Deamination of purine bases can result in accumulation of such nucleotides as ITP, dITP,
Davies, Oluwafemi+3 more
core +2 more sources
An Allosteric Inhibitory Site Conserved in the Ectodomain of P2X Receptor Channels
P2X receptors constitute a gene family of cation channels gated by extracellular ATP. They mediate fast ionotropic purinergic signaling in neurons and non-excitable cell types in vertebrates.
Ariel R. Ase+2 more
doaj +1 more source
Positron emission tomography (PET) is a non-invasive nuclear imaging technique. In PET, radiolabelled molecules decay by positron emission. The gamma rays resulting from positron annihilation are detected in coincidence and mapped to produce three ...
Leonard I. Wiebe
doaj +1 more source
Oncogenic Integration of Nucleotide Metabolism via Fatty Acid Synthase in Non-Hodgkin Lymphoma
Metabolic dysfunctions enabling increased nucleotide biosynthesis are necessary for supporting malignant proliferation. Our investigations indicate that upregulation of fatty acid synthase (FASN) and de novo lipogenesis, commonly observed in many cancers,
Dashnamoorthy Ravi+27 more
doaj +1 more source