Two complementing in vivo selection systems based on CCA-trimming exonucleases as a tool to monitor, select and evaluate enzymatic features of tRNA nucleotidyltransferases [PDF]
RNA BiologytRNA nucleotidyltransferase represents a ubiquitous and essential activity that adds the indispensable CCA triplet to the 3’-end of tRNAs. To fulfill this function, the enzyme contains a set of highly conserved motifs whose coordinated interplay is ...
Karolin Wellner +5 more
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Identification and Characterization of Mammalian Mitochondrial tRNA nucleotidyltransferases [PDF]
Journal of Biological Chemistry, 2001 The CCA-adding enzyme (ATP:tRNA adenylyltransferase or CTP:tRNA cytidylyltransferase (EC 2.7.7.25)) generates the conserved CCA sequence responsible for the attachment of amino acid at the 3′ terminus of tRNA molecules.
Tsutomu Suzuki +2 more
exaly +7 more sources
Feedback Inhibition of Bacterial Nucleotidyltransferases by Rare Nucleotide l-Sugars Restricts Substrate Promiscuity. [PDF]
J Am Chem Soc, 2023 Bacterial glycomes are rich in prokaryote-specific or “rare” sugars that are absent in mammals. Like common sugars found across organisms, rare sugars are typically activated as nucleoside diphosphate sugars (NDP-sugars) by nucleotidyltransferases.
Zheng M, Zheng MC, Kim H, Lupoli TJ.
europepmc +3 more sources
Crystal structure and functional implications of cyclic di-pyrimidine-synthesizing cGAS/DncV-like nucleotidyltransferases [PDF]
Nature Communications, 2023 Purine-containing nucleotide second messengers regulate diverse cellular activities. Cyclic di-pyrimidines mediate anti-phage functions in bacteria; however, the synthesis mechanism remains elusive.
Chia-Shin Yang +5 more
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Frontiers in Microbiology, 2018 Thermotolerant Campylobacter species C. jejuni and C. coli are actually recognized as the major bacterial agent responsible for food-transmitted gastroenteritis. The most effective antimicrobials against Campylobacter are macrolides and some, but not all
María Ugarte-ruiz +2 more
exaly +4 more sources
Molecular mechanisms of template-independent RNA polymerization by tRNA nucleotidyltransferases
Frontiers in Genetics, 2014 The universal 3'-terminal CCA sequence of tRNA is built and/or synthesized by the CCA-adding enzyme, CTP:(ATP) tRNA nucleotidyltransferase. This RNA polymerase has no nucleic acid template, but faithfully synthesizes the defined CCA sequence on the 3 ...
Kozo Tomita, Seisuke Yamashita
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Biochemical and structural bioinformatics studies of fungal CutA nucleotidyltransferases explain their unusual specificity toward CTP and increased tendency for cytidine incorporation at the 3′-terminal positions of synthesized tails [PDF]
RNA: A publication of the RNA Society, 2017 Noncanonical RNA nucleotidyltransferases (NTases), including poly(A), poly(U) polymerases (PAPs/PUPs), and C/U-adding enzymes, modify 3′-ends of different transcripts affecting their functionality and stability.
Kamil Kobyłecki +4 more
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PLoS Genetics, 2017 The emergence and spread of antibiotic resistance among Acinetobacter spp. have been investigated extensively. Most studies focused on the multiple antibiotic resistance genes located on plasmids or genomic resistance islands.
Gang Zhang +7 more
doaj +2 more sources
Polyadenylation in Arabidopsis and Chlamydomonas organelles: the input of nucleotidyltransferases, poly(A) polymerases and polynucleotide phosphorylase [PDF]
The Plant Journal, 2009 The polyadenylation-stimulated RNA degradation pathway takes place in plant and algal organelles, yet the identities of the enzymes that catalyze the addition of the tails remain to be clarified. In a search for the enzymes responsible for adding poly(A)
Sara L. Zimmer +4 more
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“ADP Sulfurylase” from Thiobacillus denitrificansIs an Adenylylsulfate:Phosphate Adenylyltransferase and Belongs to a New Family of Nucleotidyltransferases [PDF]
Journal of Biological Chemistry, 2000 During AMP-dependent sulfite oxidation by some sulfur bacteria, the liberation of sulfate from adenosine-5′-phosphosulfate (APS) is catalyzed by APS:phosphate adenylyltransferase (APAT).
Thomas Brüser +2 more
openalex +2 more sources