Results 1 to 10 of about 6,735 (123)

Bicuculline reversal of aminoglycoside O-nucleotidyltransferase EanT-1–mediated kanamycin resistance [PDF]

BMC Microbiology
Background Kanamycin, an aminoglycoside, is an effective broad-spectrum antimicrobial agent against bacterial infections. However, the clinical efficacy of aminoglycosides has been overshadowed by the emergence of resistance mechanisms involving enzyme ...
Jiahui Wang, Minghui Yu, Mingzhen Zhang, Zhihui Meng, Peitong Yang, Xingyu Cui, Ziqian Yu, Yongan Wang, Gongli Zong   +8 more
doaj   +2 more sources

Structures and mechanism of E2‐CBASS anti‐phage system [PDF]

mLife
Bacteria deploy diverse innate immune systems to combat bacteriophage infections. The cyclic‐oligonucleotide‐based anti‐phage signaling system (CBASS) is a type of innate prokaryotic immune system.
Jun Xiao, Yan Yan, Jing Li, Greater Kayode Oyejobi, Dongyang Lan, Bin Zhu, Zhiming Wang, Longfei Wang   +7 more
doaj   +2 more sources

Two complementing in vivo selection systems based on CCA-trimming exonucleases as a tool to monitor, select and evaluate enzymatic features of tRNA nucleotidyltransferases [PDF]

RNA Biology
tRNA nucleotidyltransferase represents a ubiquitous and essential activity that adds the indispensable CCA triplet to the 3’-end of tRNAs. To fulfill this function, the enzyme contains a set of highly conserved motifs whose coordinated interplay is ...
Karolin Wellner, Josefine Gnauck, Dorian Bernier, Stephan H. Bernhart, Heike Betat, Mario Mörl   +5 more
doaj   +2 more sources

Infancy‐onset diabetes caused by de‐regulated AMPylation of the human endoplasmic reticulum chaperone BiP

EMBO Molecular Medicine, 2023
Dysfunction of the endoplasmic reticulum (ER) in insulin‐producing beta cells results in cell loss and diabetes mellitus. Here we report on five individuals from three different consanguineous families with infancy‐onset diabetes mellitus and severe ...
Luke A Perera, Andrew T Hattersley, Heather P Harding, Matthew N Wakeling, Sarah E Flanagan, Ibrahim Mohsina, Jamal Raza, Alice Gardham, David Ron, Elisa De Franco   +9 more
doaj   +1 more source

The Battle for Survival: The Role of RNA Non-Canonical Tails in the Virus–Host Interaction

Metabolites, 2023
Terminal nucleotidyltransferases (TENTs) could generate a ‘mixed tail’ or ‘U-rich tail’ consisting of different nucleotides at the 3′ end of RNA by non-templated nucleotide addition to protect or degrade cellular messenger RNA.
Xianghui Wen, Ahsan Irshad, Hua Jin
doaj   +1 more source

Identification of a novel aminoglycoside O-nucleotidyltransferase AadA33 in Providencia vermicola

Frontiers in Microbiology, 2022
A novel chromosome-encoded aminoglycoside O-nucleotidyltransferase AadA33 was identified in Providencia vermicola strain P13. The AadA33 shares the highest amino acid identity of 51.28% with the function characterized AadA31.
Chunlin Feng, Chunlin Feng, Mengdi Gao, Weiyan Jiang, Weina Shi, Anqi Li, Shuang Liu, Lei Zhang, Xueya Zhang, Qiaoling Li, Hailong Lin, Junwan Lu, Junwan Lu, Kewei Li, Hailin Zhang, Yunliang Hu, Qiyu Bao, Qiyu Bao, Qiyu Bao, Xi Lin, Xi Lin   +20 more
doaj   +1 more source

The arms race between bacteria CBASS and bacteriophages

Frontiers in Immunology, 2023
The Bacterial Cyclic oligonucleotide-Based Anti-phage Signaling System (CBASS) is an innate immune system that induces cell suicide to defend against phage infections.
Lan Wang, Lan Wang, Lan Wang, Leiliang Zhang, Leiliang Zhang, Leiliang Zhang   +5 more
doaj   +1 more source

Crystal structure and functional implications of cyclic di-pyrimidine-synthesizing cGAS/DncV-like nucleotidyltransferases

Nature Communications, 2023
Purine-containing nucleotide second messengers regulate diverse cellular activities. Cyclic di-pyrimidines mediate anti-phage functions in bacteria; however, the synthesis mechanism remains elusive.
Chia-Shin Yang, Tzu-Ping Ko, Chao-Jung Chen, Mei-Hui Hou, Yu-Chuan Wang, Yeh Chen   +5 more
doaj   +1 more source

Promiscuous splicing-derived hairpins are dominant substrates of tailing-mediated defense of miRNA biogenesis in mammals

Cell Reports, 2023
Summary: Canonical microRNA (miRNA) hairpins are processed by the RNase III enzymes Drosha and Dicer into ∼22 nt RNAs loaded into an Argonaute (Ago) effector.
Seungjae Lee, David Jee, Sid Srivastava, Acong Yang, Abhinav Ramidi, Renfu Shang, Diane Bortolamiol-Becet, Sébastien Pfeffer, Shuo Gu, Jiayu Wen, Eric C. Lai   +10 more
doaj   +1 more source

Bacteriophage antidefense genes that neutralize TIR and STING immune responses

Cell Reports, 2023
Summary: Programmed cell suicide of infected bacteria, known as abortive infection (Abi), serves as an immune defense strategy to prevent the propagation of bacteriophage viruses.
Peiyin Ho, Yibu Chen, Subarna Biswas, Ethan Canfield, Alireza Abdolvahabi, Douglas E. Feldman   +5 more
doaj   +1 more source