Nature Communications, 2023 Purine-containing nucleotide second messengers regulate diverse cellular activities. Cyclic di-pyrimidines mediate anti-phage functions in bacteria; however, the synthesis mechanism remains elusive.
Chia-Shin Yang +5 more
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A Phylogeny of Bacterial RNA Nucleotidyltransferases: Bacillus halodurans Contains Two tRNA Nucleotidyltransferases [PDF]
Journal of Bacteriology, 2005 ABSTRACT We have analyzed the distribution of RNA nucleotidyltransferases from the family that includes poly(A) polymerases (PAP) and tRNA nucleotidyltransferases (TNT) in 43 bacterial species. Genes of several bacterial species encode only one member of the nucleotidyltransferase superfamily (NTSF), and if that protein functions
Patricia, Bralley +2 more
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Molecular mechanisms of template-independent RNA polymerization by tRNA nucleotidyltransferases
Frontiers in Genetics, 2014 The universal 3'-terminal CCA sequence of tRNA is built and/or synthesized by the CCA-adding enzyme, CTP:(ATP) tRNA nucleotidyltransferase. This RNA polymerase has no nucleic acid template, but faithfully synthesizes the defined CCA sequence on the 3 ...
Kozo eTomita, Seisueke eYamashita
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PLoS Genetics, 2017 The emergence and spread of antibiotic resistance among Acinetobacter spp. have been investigated extensively. Most studies focused on the multiple antibiotic resistance genes located on plasmids or genomic resistance islands.
Gang Zhang +7 more
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Identification and Characterization of Mammalian Mitochondrial tRNA nucleotidyltransferases [PDF]
Journal of Biological Chemistry, 2001 The CCA-adding enzyme (ATP:tRNA adenylyltransferase or CTP:tRNA cytidylyltransferase (EC )) generates the conserved CCA sequence responsible for the attachment of amino acid at the 3' terminus of tRNA molecules. It was shown that enzymes from various organisms strictly recognize the elbow region of tRNA formed by the conserved D- and T-loops.
T, Nagaike +6 more
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Mechanism of Mg2+-Accompanied Product Release in Sugar Nucleotidyltransferases [PDF]
Structure, 2018 The nucleotidyl transfer reaction, catalyzed by sugar nucleotidyltransferases (SNTs), is assisted by two active site Mg2+ ions. While studying this reaction using X-ray crystallography, we captured snapshots of the pyrophosphate (product) as it exits along a pocket.
Vithani, Neha +6 more
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tRNA‐nucleotidyltransferases: Highly unusual RNA polymerases with vital functions [PDF]
FEBS Letters, 2009 tRNA‐nucleotidyltransferases are fascinating and unusual RNA polymerases responsible for the synthesis of the nucleotide triplet CCA at the 3′‐terminus of tRNAs. As this CCA end represents an essential functional element for aminoacylation and translation, these polymerases (CCA‐adding enzymes) are of vital importance in all organisms.
Vörtler, Stefan, Mörl, Mario
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The Terminal Nucleotidyltransferase of Calf Thymus Nuclei
Journal of Biological Chemistry, 1966 Abstract An enzyme from calf thymus nuclei which incorporates ribonucleotides or deoxyribonucleotides into terminal positions of deoxyribonucleic acid has been purified and its mechanism of action elucidated. Distinctions between this enzyme and replicative deoxynucleotidyltransferase (DNA polymerase), the terminal deoxynucleotidyltransferase of calf ...
M E, Gottesman, E S, Canellakis
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Biochemical and structural bioinformatics studies of fungal CutA nucleotidyltransferases explain their unusual specificity toward CTP and increased tendency for cytidine incorporation at the 3′-terminal positions of synthesized tails [PDF]
RNA: A publication of the RNA Society, 2017 Noncanonical RNA nucleotidyltransferases (NTases), including poly(A), poly(U) polymerases (PAPs/PUPs), and C/U-adding enzymes, modify 3′-ends of different transcripts affecting their functionality and stability.
Kamil Kobyłecki +4 more
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Substrate Affinity Versus Catalytic Efficiency: Ancestral Sequence Reconstruction of tRNA Nucleotidyltransferases Solves an Enzyme Puzzle [PDF]
Molecular biology and evolution, 2022 In tRNA maturation, CCA-addition by tRNA nucleotidyltransferase is a unique and highly accurate reaction. While the mechanism of nucleotide selection and polymerization is well understood, it remains a mystery why bacterial and eukaryotic enzymes exhibit
Martina Hager +7 more
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