Results 31 to 40 of about 8,237 (208)

Terminal Uridylyltransferases TUT4/7 Regulate microRNA and mRNA Homeostasis

Cells, 2022
The terminal nucleotidyltransferases TUT4 and TUT7 (TUT4/7) regulate miRNA and mRNA stability by 3′ end uridylation. In humans, TUT4/7 polyuridylates both mRNA and pre-miRNA, leading to degradation by the U-specific exonuclease DIS3L2. We investigate the
Pengcheng Zhang, Mallory I. Frederick, Ilka U. Heinemann   +2 more
doaj   +1 more source

CCA-Addition Gone Wild: Unusual Occurrence and Phylogeny of Four Different tRNA Nucleotidyltransferases in Acanthamoeba castellanii

Molecular biology and evolution, 2020
tRNAs are important players in the protein synthesis machinery, where they act as adapter molecules for translating the mRNA codons into the corresponding amino acid sequence.
L. Erber, H. Betat, M. Mörl
semanticscholar   +1 more source

AGO-bound mature miRNAs are oligouridylated by TUTs and subsequently degraded by DIS3L2

Nature Communications, 2020
3′ end of microRNAs binds to the PAZ domain of Argonaute (AGO) proteins. Here the authors show that terminal nucleotidyltransferases TUT4/7 and exonuclease DIS3L2 induce tailing and decay of 3’ end exposed-microRNAs in AGO PAZ mutant expressing- or ...
Acong Yang, Tie-Juan Shao, Xavier Bofill-De Ros, Chuanjiang Lian, Patricia Villanueva, Lisheng Dai, Shuo Gu   +6 more
doaj   +1 more source

Identification and characterization of a novel aminoglycoside O-nucleotidyltransferase ANT(6)-If from Paenibacillus thiaminolyticus PATH554

Frontiers in Microbiology, 2023
BackgroundPaenibacillus thiaminolyticus, a species of genus Paenibacillus of the family Paenibacillaceae, exists widely in environments and habitats in various plants and worms, and occasionally causes human infections.
Junwan Lu, Junwan Lu, Yuning Sha, Mengdi Gao, Weina Shi, Xi Lin, Kewei Li, Qiyu Bao, Qiyu Bao, Chunlin Feng, Chunlin Feng   +10 more
doaj   +1 more source

Origin of the Genetic Code Is Found at the Transition between a Thioester World of Peptides and the Phosphoester World of Polynucleotides

Life, 2019
The early metabolism arising in a Thioester world gave rise to amino acids and their simple peptides. The catalytic activity of these early simple peptides became instrumental in the transition from Thioester World to a Phosphate World.
Hyman Hartman, Temple F. Smith
doaj   +1 more source

The complete genome sequence of hyperthermophile Dictyoglomus turgidum DSM 6724™ reveals a specialized carbohydrate fermentor

Frontiers in Microbiology, 2016
Here we report the complete genome sequence of the chemoorganotrophic, extremely thermophilic bacterium, Dictyoglomus turgidum, which is a Gram negative, strictly anaerobic bacterium. D. turgidum and D.
Phillip Brumm, Phillip Brumm, David A Mead, David A Mead, Krishna Gowda, Krishna Gowda, Frank T Robb   +6 more
doaj   +1 more source

THIOL GROUPS IN DEOXYRIBONUCLEIC ACID NUCLEOTIDYLTRANSFERASE [PDF]

Biochemical Journal, 1965
1. The inhibitory effects of iodoacetate, iodoacetamide, p-hydroxymercuribenzoate and sarkomycin were studied in a partially purified preparation of deoxyribonucleic acid nucleotidyltransferase from Landschutz ascites-tumour cells. All of these agents inhibited the activity of the enzyme, and it was shown that the inhibition exerted by the last three ...
H M, KEIR, J B, SHEPHERD
openaire   +2 more sources

Comparative Genomics of Lactococcus spp. From Global Aquaculture Outbreaks Reveals Virulence Determinants, Antibiotic Resistance, and Phage Defence Mechanisms. [PDF]

Microbiologyopen
Lactococcus petauri predominated in isolates from outbreaks of lactococcosis, suggesting a shift in disease aetiology. Genomic analysis revealed diverse virulence factors, prophage and resistance genes, while antimicrobial susceptibility testing showed uniform resistance to common antibiotics found in farming systems, emphasising the need for ...
Blanchard AM, Secker B, Atterbury RJ, Windle SJ, Dong HT, Wongkaew J, Dien LT, Huchzermeyer D, Hang'ombe BM, Senapin S.   +9 more
europepmc   +2 more sources

Structural and Molecular Basis for Resistance to Aminoglycoside Antibiotics by the Adenylyltransferase ANT(2″)-Ia

mBio, 2015
The aminoglycosides are highly effective broad-spectrum antimicrobial agents. However, their efficacy is diminished due to enzyme-mediated covalent modification, which reduces affinity of the drug for the target ribosome.
Georgina Cox, Peter J. Stogios, Alexei Savchenko, Gerard D. Wright   +3 more
doaj   +1 more source

Enzymes are enriched in bacterial essential genes.

PLoS ONE, 2011
Essential genes, those indispensable for the survival of an organism, play a key role in the emerging field, synthetic biology. Characterization of functions encoded by essential genes not only has important practical implications, such as in identifying
Feng Gao, Randy Ren Zhang
doaj   +1 more source