Antigenic Relationships Between Escherichia coli O Antigens O 149 to O 163 and Shigella O Antigens [PDF]
O antigens O 149 to O 163 are recent additions to the Escherichia coli antigenic scheme. Their antigenic relationships with Shigella serovars were examined. O antigen identity was demonstrated between E. coli O 149 and Shigella boydii 1 and between E. coli O 152 and provisional Shigella serovar 3341-55.
R. J. Gross, B. Rowe, M. Guiney
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Citrobacter O-antigens: structure of the O-antigenic polysaccharide from Citrobacter sp. 396 [PDF]
The structure of the O-specific polysaccharide moiety of the lipopolysaccharide from Citrobacter 396 was elucidated by composition, methylation, and periodate oxidation studies. The repeating unit consists of four 2-linked mannoses and one 3-linked N-acetylglucosamine.
Klaus Jann, Barbara Jann, Peter Prehm
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Reinvestigation of the structure of Brucella O-antigens [PDF]
O-Specific polysaccharides of Brucella contain two antigenic determinants, called A and M. Most of the strains express epitope A with a small amount of epitope M, whereas Brucella melitensis strain 16 M expresses longer polymer consisting mostly of M-type epitopes.
Kubler-Kielb, J., Vinogradov, E.
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Lipophilic O-antigens in Rhodospirillum tenue [PDF]
Lipopolysaccharides of eight wild-type strains of the phototrophic bacterium Rhodospirillum tenue have been analyzed. All of the lipopolysaccharides are highly lipophilic. The compositions of preparations obtained by the phenol-water or by the phenol-chloroform-petroleum ether procedure are very similar. The polysaccharide moiety, obtained by mild acid
Gerhart Drews+3 more
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RDFization of E.coli O-antigen
csv file and python code for RDFization of E.coli O-antigens in tabular data format.
Sunmyoung Lee+2 more
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Mechanism of O-antigen distribution in lipopolysaccharide [PDF]
O-antigen units are nonuniformly distributed among lipid A-core molecules in lipopolysaccharide (LPS) from gram-negative bacteria, as revealed by polyacrylamide gel electrophoresis in sodium dodecyl sulfate; the actual distribution patterns are complex, multimodal, and strain specific.
R C Goldman, F Hunt
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Identification of the lipopolysaccharide O‐antigen biosynthesis priming enzyme and the O‐antigen ligase in Myxococcus xanthus: critical role of LPS O‐antigen in motility and development [PDF]
SummaryMyxococcus xanthus is a model bacterium to study social behavior. At the cellular level, the different social behaviors of M. xanthus involve extensive cell–cell contacts. Here, we used bioinformatics, genetics, heterologous expression and biochemical experiments to identify and characterize the key enzymes in M.
María Pérez‐Burgos+4 more
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Shigella flexneriO-antigens revisited: final elucidation of the O-acetylation profiles and a survey of the O-antigen structure diversity [PDF]
Shigella flexneri is an important human pathogen causing shigellosis. Strains of S. flexneri are serologically heterogeneous and, based on O-antigens, are currently classified into 14 types. Structures of the O-antigens (O-polysaccharides) of S. flexneri have been under study since 1960s but some gaps still remained.
Bin Liu+11 more
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Characterization of the O antigen gene cluster and structural analysis of the O antigen of Francisella tularensis subsp. tularensis [PDF]
A gene cluster encoding enzymes involved in LPS O antigen biosynthesis was identified from the partial genome sequence of Francisella tularensis subsp. tularensis Schu S4. All of the genes within the cluster were assigned putative functions based on sequence similarity with genes from O antigen biosynthetic clusters from other bacteria.
Paul G. Hitchen+7 more
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The role of the O antigen in adjuvant activity of lipopolysaccharide [PDF]
Adjuvant activities of isogenic Salmonella enterica, serovar Typhimurium, O-6,7 and O-4,5,12 lipopolysaccharide (LPS), lipid A and Bordetella pertussis LPS were compared by immunizing groups of mice subcutaneously with diphtheria and tetanus toxoid vaccine alone or mixed with one of the LPS derivatives.
Rose-Marie Ölander, Marjatta Nurminen
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