Results 91 to 100 of about 14,685,807 (300)
Modeling CRISPR-Cas13d on-target and off-target effects using machine learning approaches
, 2023 A major challenge in the application of the CRISPR-Cas13d system is to accurately predict its guide-dependent on-target and off-target effect. Here, we perform CRISPR-Cas13d proliferation screens and design a deep learning model, named DeepCas13, to ...
Cheng, Xiaolong +21 more
core +1 more source
Pediatric Blood &Cancer, EarlyView.ABSTRACT Background Cytomegalovirus (CMV) is a recognized trigger of immune thrombocytopenia (ITP); however, its incidence and impact on disease course in children remain controversial. Therefore, we aimed to characterize the clinical course of pediatric patients with CMV‐associated ITP.
Oded Gilad +9 more
wiley +1 more source
Prediction-based highly sensitive CRISPR off-target validation using target-specific DNA enrichment
, 2020 Off-target mutations that occur at a frequency below 0.5% can be difficult to detect.
J K Hur +17 more
core +1 more source
Pediatric Blood &Cancer, EarlyView.ABSTRACT Background Reduced bone mineral density (BMD) has been reported in survivors of childhood and young adult acute lymphoblastic leukaemia (ALL); however, data from cohorts treated with contemporary protocols and including matched controls remain limited.
Karen Schow Jensen +10 more
wiley +1 more source
Structure-Guided Control of siRNA Off-Target Effects [PDF]
Journal of the American Chemical Society, 2016 Short interfering RNAs (siRNAs) are promising therapeutics that make use of the RNA interference (RNAi) pathway, but liabilities arising from the native RNA structure necessitate chemical modification for drug development. Advances in the structural characterization of components of the human RNAi pathway have enabled structure-guided optimization of ...
Suter, Scott R +7 more
openaire +4 more sources
Off-target pileups from the whole benchmark experiment data set.
Off-target pileups from the whole benchmark experiment data set.
Hiroki Shiomi (718724) +13 more
core +1 more source
Picky predicted siRNA off-target genes.
, 2013 List of off-target genes predicted by Picky for the bad siRNAs and four commercial siRNAs. Only detectable off-target genes (Cp values smaller than 30) are labelled with their qPCR measured Cp values. Off-target genes whose Cp value is larger than 30 are
Peng Liu (120506) +2 more
core +1 more source
Pediatric Blood &Cancer, EarlyView.ABSTRACT Background The Standards for Psychosocial Care for Children with Cancer and Their Families guide the provision of comprehensive psychosocial care. Parallel to research characterizing implementation of the Standards from the perspective of providers, this study examines implementation of the Standards from the perspective of parents of youth ...
Emily M. Pariseau +8 more
wiley +1 more source
, 2019 Additional file 1: Figure S1. The same off-target effect was found in half of the offspring of mice carrying off-target locus. (A) Sanger Sequencing of the target PCR products (short band) of #1 off-target locus (Trp53 pseudogene) showed overlapping ...
Junchao Dong (7582535) +4 more
core +1 more source
Pediatric Blood &Cancer, EarlyView.ABSTRACT Pediatric intramedullary low‐grade gliomas (PIMLGGs) are rare neoplasms that present unique clinical and management challenges. Although surgery remains the primary tool for tissue diagnosis and decompression, complete gross‐total resection is frequently unfeasible due to the infiltrative nature of these lesions.
Olga M. Sergeenko +5 more
wiley +1 more source