Results 71 to 80 of about 13,860,386 (295)

Early Body Mass Index z‐Score Change and Resolution of Severe Malnutrition in Children With Sickle Cell Anemia in a Low‐Income Setting: A Prospective Single‐Arm Extension Study

Pediatric Blood &Cancer, EarlyView.
ABSTRACT Background Children with sickle cell anemia (SCA) in low‐income settings are at risk of severe malnutrition, but optimal nutritional management has not been established. We evaluated an intensified ready‐to‐use therapeutic food (RUTF) regimen in children with persistent severe malnutrition after initial treatment and assessed whether early ...
Safiya Gambo, Shehu Umar Abdullahi, Hassan Adam Murtala, Halima Kabir, Khadija A. Shamsu, Sari A. Acra, Virginia A. Stallings, Mark Rodeghier, Michael R. DeBaun, Lauren J. Klein   +9 more
wiley   +1 more source

Comparative analysis of antibody-mediated loss-of-function versus gene knock-out and knock-down

SLAS Discovery
In this study we compare three methods for manipulating cell function: RNA interference (RNAi), CRISPR-Cas9 gene knock-out, and antibody-mediated loss-of-function.
Marie Buck-Wiese, Sally Liechocki, Holger Erfle, Vytaute Starkuviene   +3 more
doaj   +1 more source

Analysis of Single Nucleotide Variants in CRISPR-Cas9 Edited Zebrafish Exomes Shows No Evidence of Off-Target Inflation

Frontiers in Genetics, 2019
Therapeutic applications of CRISPR-Cas9 gene editing have spurred innovation in Cas9 enzyme engineering and single guide RNA (sgRNA) design algorithms to minimize potential off-target events. While recent work in rodents outlines favorable conditions for
Marie R. Mooney, Erica E. Davis, Erica E. Davis, Erica E. Davis, Nicholas Katsanis, Nicholas Katsanis, Nicholas Katsanis   +6 more
doaj   +1 more source

Off-Target Cannabinoid Effects Mediated by GPR55 [PDF]

Pharmacology, 2012
Given the vast therapeutic potential of the endocannabinoid system, the revelation of a novel cannabinoid-sensitive target was treated with great excitement. The orphan G-protein coupled receptor 55 (GPR55) was initially touted as a novel cannabinoid target in early industrial patent literature.
openaire   +3 more sources

Central Nervous System Tumors Among Infants in Canada: A Report From CYP‐C

Pediatric Blood &Cancer, EarlyView.
ABSTRACT Background Central nervous system (CNS) tumors in infants are rare, pose unique clinical challenges, and lack large‐scale evidence‐based data to guide management. This study seeks to describe CNS tumors in Canadian infants and to compare their outcomes with those of older children.
Samuel Sassine, Hallie Coltin, Sylvia Cheng, Lucie Lafay‐Cousin, Vanan Issai Magimairajan, Craig Erker, Stéphanie Vairy, Lynette Bowes, Adam Fleming, Donna Johnston, Sarah McKillop, Roona Sinha, Julie Bennett, Christina Coleman, Laura Wheaton, Shayna Zelcer, Samuele Renzi, Thai Hoa Tran   +17 more
wiley   +1 more source

Structural basis for Cas9 off-target activity [PDF]

, 2022
The target DNA specificity of the CRISPR-associated genome editor nuclease Cas9 is determined by complementarity to a 20-nucleotide segment in its guide RNA.
Allain, Frédéric H-T; https://orcid.org/, Donohoue, Paul D, Jinek, Martin; https://orcid.org/, Pacesa, Martin; https://orcid.org/, Cléry, Antoine; https://orcid.org/, Palermo, Giulia; https://orcid.org/, Arantes, Pablo R; https://orcid.org/, Cameron, Peter, Saha, Aakash; https://orcid.org/, Irby, Matthew J, Lin, Chun-Han, Bargsten, Katja   +11 more
core   +1 more source

Structure-Guided Control of siRNA Off-Target Effects [PDF]

Journal of the American Chemical Society, 2016
Short interfering RNAs (siRNAs) are promising therapeutics that make use of the RNA interference (RNAi) pathway, but liabilities arising from the native RNA structure necessitate chemical modification for drug development. Advances in the structural characterization of components of the human RNAi pathway have enabled structure-guided optimization of ...
Suter, Scott R, Sheu-Gruttadauria, Jessica, Schirle, Nicole T, Valenzuela, Rachel, Ball-Jones, Alexi A, Onizuka, Kazumitsu, MacRae, Ian J, Beal, Peter A   +7 more
openaire   +4 more sources

Impact of Radiation Therapy on Physical and Psychosocial Health of Adolescents and Young Adults: A Joint Report From the Children's Oncology Group AYA and Radiation Oncology Committees

Pediatric Blood &Cancer, EarlyView.
ABSTRACT Rates of cancer among adolescents and young adults (AYA), age 15–39 years, are increasing. Consequently, radiation oncologists are treating more AYAs who have diagnoses spanning both pediatric and adult practices. Compared to pediatric and older adult patients, AYAs face a unique set of challenges.
Hesham Elhalawani, Rachel K. Peterson, Avani D. Rao, Laurie J. McKenzie, Ahmed Magdy Elmotayam, Leslie Chang, Michael Roth, Susan L. McGovern   +7 more
wiley   +1 more source

Off-targets analysis of sgRNAs.

, 2019
Surveyor analysis of the top 5 predicted off-targets for each sgRNA used. Panel shows amplified sequence of target and 3 representative off-targets, non-treated and treated with T7 endonuclease I.
Elena Vuelta (6685883), Fermín Sánchez- Guijo (6685901), Lucía Méndez (614092), Marta Martín-Izquierdo (6685892), Sandra Pérez- Ramos (6685886), Ignacio García-Tuñón (79373), Verónica Alonso-Pérez (6685880), Jesús María Hernández-Sánchez (6685889), Jesús María Hernández-Rivas (102959), Manuel Sánchez-Martín (50259), Raquel Saldaña (6685895), María Herrero (345477), Julián Sevilla (6685898)   +12 more
core   +1 more source

Social Functioning Within the First Years After Pediatric Brain Tumor Diagnosis and the Relationship With Family Psychosocial Risk

Pediatric Blood &Cancer, EarlyView.
ABSTRACT Background Survivors of pediatric brain tumors (PBTs) can experience long‐term social difficulties, impacting quality of life. Beyond medical and environmental factors, family psychosocial risk may play a role in social outcomes by shaping the caregiving environment and may provide intervention options.
Renske H. Houben, Marisa H. Huisman, Martha A. Grootenhuis, Femke K. Aarsen, Marita Partanen   +4 more
wiley   +1 more source