Results 101 to 110 of about 14,685,807 (300)

CRISPR/Cas9 from bench to bedside: what clinicians need to know before application?

Military Medical Research, 2020
In October 2020, Dr. Emmanuelle Charpentier and Dr. Jennifer Doudna won the Nobel Prize in Chemistry for their pioneering work in precise genome editing using the CRISPR technology.
Zi-Qing Li, Chao-Hong Li
doaj   +1 more source

Exercise Capacity, Physical Activity, Fatigue, and Neurocognitive Function in Adult Survivors of Childhood Acute Lymphoblastic Leukemia

Pediatric Blood &Cancer, EarlyView.
ABSTRACT Background Adult survivors of childhood acute lymphoblastic leukemia (ALL) are at risk of reduced exercise capacity, decreased physical activity levels, persistent fatigue, and neurocognitive deficits. The aim was to investigate exercise capacity and physical activity levels of adult survivors of childhood ALL and their associations with ...
Laura Jess, Gustaf Glavå, Simon Skau, Hans‐Georg Kuhn, Maria Bäck, Marianne Jarfelt   +5 more
wiley   +1 more source

Prime Editing Exhibits Limited Genome-Wide Off-Target Effects in Cellular and Embryonic Gene Editing

Cells
Prime editing (PE) is a precise genome-editing technology that avoids double-strand breaks, holding great promise for clinical and agricultural applications.
Jitan Zheng, Mingdi Wu, Xueyan Wang, Zhenrui Zuo, Chikai Zhou, Erwei Zuo, Yangqing Lu   +6 more
doaj   +1 more source

Encountering unpredicted off-target effects of pharmacological inhibitors [PDF]

Journal of Biochemistry, 2011
With the emergence of chemical biology, the use of pharmacological inhibitors in biological research has been expanding. SP600125 is a low-molecular weight compound that has been widely used to inhibit c-Jun-N-terminal kinase (JNK). A recent publication by Tanemura et al. (J. Biochem.
openaire   +2 more sources

“We Cannot Talk as a Family”: Complexities of Survivorship Communication Among Hispanic/Latino Young Adult Survivors of Childhood Cancer, Parents, and Clinicians

Pediatric Blood &Cancer, EarlyView.
ABSTRACT Background Young adult childhood cancer survivors (YA‐CCS) have an increased risk of treatment‐related health complications throughout their lives. Lifelong survivorship care is recommended, yet many YA‐CCS experience disruptions in care during pediatric‐to‐adult transitions and do not receive recommended survivorship care.
Kevin Shanchen Pu, Nicole Fernández‐Viña, Briana C. Martin‐Villa, Daniela Ramirez, Rebecca M. Lewinsohn, Mary Smith, Heidi Boynton, Lidia Schapira, Stephanie M. Smith   +8 more
wiley   +1 more source

Identifying Patterns of Hospitalizations Among Childhood Cancer Survivors: A Population‐Based Study

Pediatric Blood &Cancer, EarlyView.
ABSTRACT Background Childhood cancer survivors (CCSs) experience more hospitalizations than the general population. We aimed to identify distinct hospitalization patterns among Queensland CCSs and the associated characteristics. Methods Cases (aged ≤14 years) diagnosed between 1995 and 2019 while residents of Queensland were identified through the ...
Hai Pham, Teyl Engstrom, Peter Baade, Natalie Bradford, Sarah Pont, Louise Marquart‐Wilson, Natasha Nassar, Jason D. Pole   +7 more
wiley   +1 more source

Screening, optimization and artificial recombination of dsRNA fragments for RNAi-mediated pest resistance in Apolygus lucorum

Frontiers in Insect Science
RNA interference (RNAi) is an eco-friendly strategy for pest management, with double-stranded RNA (dsRNA) as the core functional component. In this study, three RNAi target genes (Ubx, wupA and Dpp) with strong lethal effects on Apolygus lucorum were ...
Juan Luo, Qianqian Guo, Qianqian Guo, Ruyi Hu, Mengjun Zhang, Guirong Wang, Guirong Wang, Bin Yang   +7 more
doaj   +1 more source

Classical Complement Pathway Inhibition in a Pediatric Patient With Refractory Immune Thrombocytopenia

Pediatric Blood &Cancer, EarlyView.
ABSTRACT Pediatric refractory immune thrombocytopenia (rITP) is generally characterized by persistent thrombocytopenia and failure to achieve a sustained response to multiple standard therapies. We report a 4‐year‐old female with congenital tufting enteropathy, post‐intestinal transplant patient on immunosuppressants, who developed severe rITP ...
Sarah Hsieh, Susmita Sarangi, Caileigh Pudela, Catherine M. Broome, Tara K. Suntum   +4 more
wiley   +1 more source

Molecular mechanisms of AcrVA-mediated Cas12a inhibition and their potential applications

Frontiers in Microbiology
CRISPR-Cas12a is widely used for genome editing because it is simple, efficient, and suitable for multiplex targeting. However, strong or prolonged Cas12a activity can increase unwanted cleavage at off-target sites.
Yong Jun Kang, Hyun Ho Park, Hyun Ho Park   +2 more
doaj   +1 more source

siRNA off-target effects can be reduced at concentrations that match their individual potency. [PDF]

, 2011
Small interfering RNAs (siRNAs) are routinely used to reduce mRNA levels for a specific gene with the goal of studying its function. Several studies have demonstrated that siRNAs are not always specific and can have many off-target effects.
Michael E. Schaffer, Seymour Albert B., Steven A. Haney, Schaffer Michael E., Steven A Haney, Subramanian Romesh R., Hughes Jason D., Zhao Juan, Daniel R Caffrey, Daniel R. Caffrey, Zhili Song, Haney Steven A., Caffrey Daniel R., Song Zhili, Juan Zhao, Michael E Schaffer, Albert B. Seymour, Jason D Hughes, Romesh R Subramanian, Jason D. Hughes, Albert B Seymour, Romesh R. Subramanian   +21 more
core   +1 more source