Results 151 to 160 of about 1,634 (252)

Cell‐in‐Bead‐in‐Droplet Platform for pH‐Based Microfluidic Screening of Ureolytic Bacteria

Small, EarlyView.
Efficient high‐throughput microbial screening in droplet microfluidics is often challenged by chemical crosstalk between droplets. This is particularly difficult in pH‐changing biological processes, for which more effective biochemical assays are needed.
Diego Giovanoli, Nadia Enrriquez, Anton Kan, Mathias Steinacher, Raphael Buess, Tomas Pena Müller, Stavros Stavrakis, Andrew deMello, André R. Studart   +8 more
wiley   +1 more source

Diving Deeper Into Mechanisms of Acrylamide‐Induced Toxicity: RNA Sequencing Reveals Transcriptomic Alteration and Retrotransposon Expression in Drosophila melanogaster

Environmental Toxicology, EarlyView.
ABSTRACT Given the inevitability of human and animal exposure to acrylamide, there is increasing concern regarding its potential health risks. While a number of molecular mechanisms have been proposed, the complexity of acrylamide toxicological pathways and interactions remains incompletely characterized.
Oluwabukola Mary Farodoye, Titilayomi Ayomide Otenaike, Daniela Moreira Mombach, Monica Medeiros Silva, Amos Olalekan Abolaji, Elgion Lucio Da Silva Loreto   +5 more
wiley   +1 more source

Hevea brasiliensis seeds: A novel sustainable feedstock for ethanol production

Vietnam Journal of Chemistry, EarlyView.
Abstract The rubber tree (Hevea brasiliensis) is cultivated on a large scale mainly for the extraction of latex in Brazil and Southeast Asia, also generating large quantities of seeds. These seeds currently do not have large‐scale applications and may thus be considered as a residue.
Giovano Tochetto, Fernanda Oliveira Lima, Letiére Cabreira Soares, Dalila Moter Benvegnú, André Lazarin Gallina   +4 more
wiley   +1 more source

Optimization of lipid extraction from the oleaginous yeasts Rhodotorula glutinis and Lipomyces kononenkoae. [PDF]

AMB Express, 2018
Vasconcelos B, Teixeira JC, Dragone G, Teixeira JA.   +3 more
europepmc   +1 more source

CRISPR/Cas9 Genome Engineering in Non‐Conventional Oleaginous Yeasts: Applications, Challenges, and Prospects

Yeast, EarlyView.
ABSTRACT Given the biotechnological potential of yeast‐derived oils for oleochemical production, genes encoding lipid metabolism enzymes are key targets for metabolic engineering. Genetic engineering tools such as Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9, Transcription Activator‐Like Effector Nucleases (TALENs), Zinc ...
Rodrigo Gonçalves Dias, Fernanda Pinheiro Moreira Freitas, Eduardo Luís Menezes de Almeida, Luciano Gomes Fietto, Agustin Zsögön, Wendel Batista da Silveira   +5 more
wiley   +1 more source

Where's the beef? The feminisation of weight‐loss dieting in Britain and Scandinavia c.1890–1925

Gender &History, EarlyView.
Abstract Representations of the slim body have traditionally been at the centre of scholarly interest in dieting culture, whereas food often remains a shadowy presence compared with more persistent themes of body discipline, slenderness and anti‐fat messages.
Emma Hilborn
wiley   +1 more source

Functional validation of a white pupae minimal gene construct in Ceratitis capitata (Diptera: Tephritidae)

Insect Science, EarlyView.
An intronless version of the white pupae (wp) gene was engineered to restore the wild type brown puparium color in white pupae phenotype mutants of the Mediterranean fruit fly, Ceratitis capitata. Functionality of the minimal gene version (mini‐wp) was verified in vivo, as one copy of mini‐wp successfully restored the wild type phenotype in homozygous ...
Lucas Henrique Figueiredo Prates, Roswitha A. Aumann, Inga Sievers, Tanja Rehling, Marc F. Schetelig   +4 more
wiley   +1 more source

CRISPR/Cas9‐mediated mutagenesis of the white‐eye gene in the tephritid pest Bactrocera zonata

Insect Science, EarlyView.
Abstract Bactrocera zonata is a highly invasive agricultural pest that causes extensive damage to fruit crops. The Sterile Insect Technique (SIT), a species‐specific and environmentally friendly pest control method, significantly benefits from the availability of Genetic Sexing Strains (GSSs) that enable efficient mass production of males for sterile ...
Albert Nazarov, Tamir Partosh, Flavia Krsticevic, Dimitris Rallis, Yael Arien, Guy Ostrovsky, Reut Madar Kramer, Eyal Halon, Alfred M. Handler, Simon W. Baxter, Yoav Gazit, Kostas D. Mathiopoulos, Gur Pines, Philippos A. Papathanos   +13 more
wiley   +1 more source

DsiRNA‐mediated silencing of Ceratitis capitata transformer or transformer‐2 leads to masculinization of XX embryos and systemic gene silencing in ovaries

Insect Science, EarlyView.
We carried out functional studies using Dicer‐substrate small interfering RNAs (DsiRNAs), targeting the sex determination genes Cctransformer (Cctra) and Cctransformer‐2 (Cctra‐2) in the Mediterranean fruit fly (Ceratitis capitata). Microinjection of DsiRNAs into XX embryos (0‐1 h old) rapidly triggered the expression of male‐specific Cctra isoforms ...
Gennaro Volpe, Sarah Maria Mazzucchiello, Domenico De Falco, David Torrente, Stefania Liguori, Noemi Rosati, Dora Baccaro, Michela Mazzeo, Fulvio Bertolotto, Harshini Sangle, Ennio Giordano, Angela Carfora, Francesca Lucibelli, Marianna Varone, Paola Di Lillo, Hugo Diego Perdomo, Mariangela Bonizzoni, Serena Aceto, Marco Salvemini, Molly Duman‐Scheel, Giuseppe Saccone   +20 more
wiley   +1 more source

CRISPR/Cas9‐based white pupae mutant lines in Bactrocera spp. for sterile insect technique applications

Insect Science, EarlyView.
Bactrocera fruit flies are significant horticultural pests that cause major economic losses. A “neoclassical approach” incorporating genome editing via CRISPR/Cas9 to develop genetic sexing strains (GSS) could render the sterile insect technique (SIT) against these pests more efficient and cost‐effective.
Chrysanthi Ioannidou, Maria‐Eleni Gregoriou, Marc F. Schetelig, Elena Drosopoulou, Kostas D. Mathiopoulos, Kostas Bourtzis   +5 more
wiley   +1 more source