Results 1 to 10 of about 129,877 (153)

Light-generated oligonucleotide arrays for rapid DNA sequence analysis. [PDF]

Proceedings of the National Academy of Sciences, 1994
In many areas of molecular biology there is a need to rapidly extract and analyze genetic information; however, current technologies for DNA sequence analysis are slow and labor intensive. We report here how modern photolithographic techniques can be used to facilitate sequence analysis by generating miniaturized arrays of densely packed ...
Ann C. Pease, Dennis Solas, Elizabeth J. Sullivan, Mark T.D. Cronin, Christopher P. Holmes, Stephen P. A. Fodor   +5 more
openalex   +3 more sources

Enhanced high density oligonucleotide array-based sequence analysis using modified nucleoside triphosphates [PDF]

Nucleic Acids Research, 1998
Pairs of high density oligonucleotide arrays (DNA chips) consisting of >96 000 oligonucleotides were designed to screen the entire 5.53 kb coding region of the hereditary breast and ovarian cancer BRCA1 gene for all possible sequence changes in the homozygous and heterozygous states.
J. Hacia
openalex   +4 more sources

Optimizing copy number variation analysis using genome-wide short sequence oligonucleotide arrays [PDF]

Nucleic Acids Research, 2010
The detection of copy number variants (CNV) by array-based platforms provides valuable insight into understanding human diversity. However, suboptimal study design and data processing negatively affect CNV assessment. We quantitatively evaluate their impact when short-sequence oligonucleotide arrays are applied (Affymetrix Genome-Wide Human SNP Array 6.
Derek A. Oldridge, Samprit Banerjee, Sunita R. Setlur, Andrea Sboner, Francesca Demichelis   +4 more
openalex   +7 more sources

Rapid p53 sequence analysis in primary lung cancer using an oligonucleotide probe array [PDF]

Proceedings of the National Academy of Sciences, 1999
The p53 gene was sequenced in 100 primary human lung cancers by using direct dideoxynucleotide cycle sequencing and compared with sequence analysis by using the p53 GeneChip assay. Differences in sequence analysis between the two techniques were further evaluated to determine the accuracy and limitations of each method.
Steven A. Ahrendt, Sarel Halachmi, John T. Chow, Wu Li, Naomi Halachmi, Stephen C. Yang, Scott L. Wehage, Jin Jen, David Sidransky   +8 more
openalex   +4 more sources

Effective primer design for genotype and subtype detection of highly divergent viruses in large scale genome datasets [PDF]

BMC Bioinformatics
Identification of microorganisms in a biological sample is a crucial step in diagnostics, pathogen screening, biomedical research, evolutionary studies, agriculture, and biological threat assessment.
Burak Demiralay, Tolga Can
doaj   +2 more sources

Sequencing analysis of HPV-other type on an HPV DNA chip [PDF]

Obstetrics & Gynecology Science, 2018
ObjectivesTo identify the specific human papillomavirus (HPV) genotypes from HPV-other type on an HPV DNA chip test by sequencing.MethodsAmong 13,600 women undergoing a routine gynecology examination including Pap smear and/or HPV test by DNA chip test ...
Min-Jeong Kim, Jin Ju Kim, Sunmie Kim
doaj   +1 more source

Identification of genomic imbalances in oral clefts

Jornal de Pediatria, 2021
Objective: This article presents a clinical and cytogenomic approach that focuses on the diagnosis of syndromic oral clefts (OCs). Methods: The inclusion criteria were individuals with OC presenting four or more minor signs and no major defects (non ...
Elaine Lustosa-Mendes, Ana P. dos Santos, Társis P. Vieira, Erlane M. Ribeiro, Adriana A. Rezende, Agnes C. Fett-Conte, Denise P. Cavalcanti, Têmis M. Félix, Isabella L. Monlleó, Vera Lúcia Gil-da-Silva-Lopes   +9 more
doaj   +1 more source

Systematic validation and atomic force microscopy of non-covalent short oligonucleotide barcode microarrays. [PDF]

PLoS ONE, 2008
BACKGROUND: Molecular barcode arrays provide a powerful means to analyze cellular phenotypes in parallel through detection of short (20-60 base) unique sequence tags, or "barcodes", associated with each strain or clone in a collection.
Michael A Cook, Chi-Kin Chan, Paul Jorgensen, Troy Ketela, Daniel So, Mike Tyers, Chi-Yip Ho   +6 more
doaj   +1 more source

Genomic Profiling of Liver Cancer [PDF]

Genomics & Informatics, 2013
Development of liver cancers is driven largely by genomic alterations that deregulate signaling pathways, influencing growth and survival of cancer cells. Because of the hundreds or thousands of genomic/epigenomic alterations that have accumulated in the
Ju-Seog Lee
doaj   +1 more source

GSMA: Gene Set Matrix Analysis, An Automated Method for Rapid Hypothesis Testing of Gene Expression Data [PDF]

Bioinformatics and Biology Insights, 2007
Background Microarray technology has become highly valuable for identifying complex global changes in gene expression patterns. The assignment of functional information to these complex patterns remains a challenging task in effectively interpreting data and correlating results from across experiments, projects and laboratories. Methods which allow the
James P. Hamilton, Yuriko Mori, Takatsugu Kan, Stefan David, Jian Yang, Alexandru Olaru, John M. Abraham, Stephen J. Meltzer, Fumiaki Sato, Zhe Jin, Bogdan C. Paun, Yulan Cheng, Rachana Agarwal, Tetsuo Ito   +13 more
openaire   +35 more sources