Results 11 to 20 of about 23,719,178 (251)

Analysis of oligonucleotide array experiments with repeated measures using mixed models [PDF]

BMC Bioinformatics, 2004
Background Two or more factor mixed factorial experiments are becoming increasingly common in microarray data analysis. In this case study, the two factors are presence (Patients with Alzheimer's disease) or absence (Control) of the disease, and brain ...
Getchell Thomas V, Wood Constance L, Li Hao, Getchell Marilyn L, Stromberg Arnold J   +4 more
doaj   +2 more sources

Advances in antisense oligonucleotide development for target identification, validation, and as novel therapeutics [PDF]

, 2008
Antisense oligonucleotides (As-ODNs) are single stranded, synthetically prepared strands of deoxynucleotide sequences, usually 18–21 nucleotides in length, complementary to the mRNA sequence of the target gene.
Alirio J. Melendez, Mansoor, Moizza, Moizza Mansoor, Melendez, Alirio J.   +3 more
core   +8 more sources

Rapid Exonuclease-Based De Novo Sequencing Strategy for Long Oligonucleotides via Liquid-Phase Ion Trap Coupled With Mass Spectrometry. [PDF]

Adv Sci (Weinh)
A liquid‐phase ion trap (LPIT) coupled with ESI‐MS enables rapid, high‐fidelity de novo sequencing of long oligonucleotides in ∼30 min. By applying an electric field to dissociate metal adducts in solution, LPIT delivers clean spectra, superior mass accuracy, and full sequence coverage, surpassing MALDI‐TOF and LC‐MS.
Lu S, Hong J, Fu X, Wang D, Gao Y, Xu W.
europepmc   +2 more sources

Augmenting and Assaying Nav1.1 Protein Quantity for Dravet Syndrome Therapy. [PDF]

Ann Clin Transl Neurol
ABSTRACT Dravet Syndrome (DS) is a developmental and epileptic encephalopathy predominantly caused by heterozygous loss‐of‐function variants in SCN1A, which encodes Nav1.1. Conserved upstream open reading frames (uORFs) in SCN1A were validated to regulate translation in reporter assays, demonstrating the therapeutic viability of increasing Nav1.1 from ...
Saravanan A, Safran J, Loughman A, Lin G, Vaid S, Allababidi D, Tessier PM, Dang LT.   +7 more
europepmc   +2 more sources

Evaluation of micropillar array columns for chromatographic separation of phosphorothioated oligonucleotides and their diastereomers

Analytical Science Advances, 2021
Chromatographic analysis of therapeutic oligonucleotides is challenging due to the presence of closely related impurities, degradants or metabolites and due to the presence of phosphorothioate bonds, which introduce chiral centers.
Lieve Dillen, Tiny Deschrijver, Kurt Van Mol, Jeff Op de Beeck, Paul Jacobs, Filip Cuyckens   +5 more
doaj   +1 more source

A Brassica exon array for whole-transcript gene expression profiling [PDF]

, 2010
Affymetrix GeneChip® arrays are used widely to study transcriptional changes in response to developmental and environmental stimuli. GeneChip® arrays comprise multiple 25-mer oligonucleotide probes per gene and retain certain advantages over direct ...
King Graham J., Broadley, MR, May Sean T., John P. Hammond, Bowen, H. C., Bowen, HC, Hammond, J. P., Lochlainn, Seosamh O., Neil S Graham, Philip J White, Graham J. King, Broadley, Martin R., Ó Lochlainn Seosamh, May, ST, Hammond, John P., Graham, NS, Love Christopher G., Christopher G Love, Helen C. Bowen, Graham, N. S., Sean T. May, Philip J. White, Christopher G. Love, Graham, Neil S., O Lochlainn, S., Hammond John P., White, PJ, Graham J King, Ó Lochlainn, Seosamh, May, S. T., Hammond, JP, Love, Christopher G., Love, CG, Helen C Bowen, John P Hammond, Seosamh O Lochlainn, King, GJ, King, G. J., King, Graham J., Broadley, M. R., Broadley Martin R., May, Sean T., Bowen Helen C., Martin R. Broadley, Sean T May, Martin R Broadley, White, P. J., King , Graham J., Love, C. G., Neil S. Graham, White Philip J., White, Philip J., Graham Neil S., Lochlainn, SO, Bowen, Helen C.   +54 more
core   +1 more source

Detection of minority variants within bovine respiratory syncytial virus populations using oligonucleotide-based microarrays [PDF]

, 2008
Microarray technology, originally developed for highly parallel examination of gene expression is regarded as a potential tool in prognosis and diagnosis.
Leberre, Véronique, LE BERRE, Véronique, Baranowski, Eric, François, Jean Marie, Deplanche, Martine, Trouilh, Lidwine   +5 more
core   +1 more source

Expresión específica de los genes de la respuesta inflamatoria en subpoblaciones de macrófagos.

Biomédica: revista del Instituto Nacional de Salud, 2005
Como brazo efectivo de la respuesta inmune, los macrófagos reconocen, fagocitan y destruyen patógenos potenciales. También, coordinan respuestas adicionales del hospedero mediante la síntesis de un amplio rango de mediadores de la inflamación que, en ...
Vera María Ripoll, David Hume, Marta Raquel Fontanilla   +2 more
doaj   +1 more source

Optimising the analysis of transcript data using high density oligonucleotide arrays and genomic DNA-based probe selection [PDF]

, 2007
Background: Affymetrix GeneChip arrays are widely used for transcriptomic studies in a diverse range of species. Each gene is represented on a GeneChip array by a probe-set, consisting of up to 16 probe-pairs. Signal intensities across probe-pairs within
Sean T May, Martin R Broadley, John P Hammond, Graham Neil S, Neil S Graham, Philip J White, Broadley Martin R, Graham, Neil S., White, Philip J., Hammond John P, White Philip J, May Sean T, Broadley, Martin R., Hammond, John P., May, Sean T.   +14 more
core   +1 more source

Using genomic DNA-based probe-selection to improve the sensitivity of high-density oligonucleotide arrays when applied to heterologous species [PDF]

, 2005
High-density oligonucleotide (oligo) arrays are a powerful tool for transcript profiling. Arrays based on GeneChip® technology are amongst the most widely used, although GeneChip® arrays are currently available for only a small number of plant and animal
Craigon, David J., Townsend Henrik J, Sean T May, Emmerson Zoe F, Emmerson, Zoe F., Townsend, Henrik J., Martin R Broadley, John P Hammond, Henrik J Townsend, Janet Higgins, Philip J White, Broadley Martin R, Higgins Janet, Higgins, Janet, White, Philip J., Hammond John P, White Philip J, Craigon David J, May Sean T, Broadley, Martin R., Hammond, John P., David J Craigon, May, Sean T., Zoe F Emmerson   +23 more
core   +1 more source