The Polymorphisms of Oligonucleotide Probes in Wheat Cultivars Determined by ND-FISH [PDF]
Molecules, 2019 Non-denaturing fluorescence in situ hybridization (ND-FISH) has been used to distinguish wheat chromosomes and to detect alien chromosomes in the wheat genome.
Tianheng Ren +9 more
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An electrophoretic mobility shift assay with chemiluminescent readout to evaluate DNA-targeting oligonucleotide-based probes. [PDF]
PLoS ONEA comprehensive and user-friendly method for evaluating recognition of double-stranded DNA (dsDNA) targets by oligonucleotide-based probes is presented. Thus, dsDNA-targeting probes such as single-stranded locked nucleic acids (LNAs) and double-stranded ...
Michaela E Everly +3 more
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A novel colorimetric DNA sensor for rapid detection of feline panleukopenia virus using ZnFe2O4 nanoparticles and TMB modulation [PDF]
Scientific ReportsA novel colorimetric genosensor was developed for the detection of feline panleukopenia virus (FPV) DNA, based on the peroxidase-like activity of ZnFe2O4 (ZFO) nanoparticles.
Zahra Abdoli +2 more
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Caspase-Activated Oligonucleotide Probe [PDF]
Bioconjugate Chemistry, 2020 Light-activated ("caged") oligonucleotides provide a strategy for modulating the activity of antisense oligos, siRNA, miRNA, aptamers, DNAzymes, and mRNA-capturing probes with high spatiotemporal resolution. However, the near-UV and visible wavelengths that promote these bond-breaking reactions poorly penetrate living tissue, which limits some ...
Linlin Yang +2 more
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Universal oligonucleotide microarray for sub-typing of Influenza A virus. [PDF]
PLoS ONE, 2011 A universal microchip was developed for genotyping Influenza A viruses. It contains two sets of oligonucleotide probes allowing viruses to be classified by the subtypes of hemagglutinin (H1-H13, H15, H16) and neuraminidase (N1-N9).
Vladimir A Ryabinin +5 more
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Protein detection using oligonucleotide probes [PDF]
Nucleic Acids Symposium Series, 2009 We developed a new nucleic acid-based fluorescence probe for protein detection. The method is based on the scission of an aptamer into two probes, which are then attached with a chemically reactive fluorogenic compound. The protein-dependent association of the two probes accelerates a chemical reaction and indicates the presence of the target protein ...
Aya, Shibata +4 more
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Prider: multiplexed primer design using linearly scaling approximation of set coverage
BMC Bioinformatics, 2022 Background Designing oligonucleotide primers and probes is one of the key steps of various laboratory experiments such as multiplexed PCR or digital multiplexed ligation assays.
Niina Smolander +2 more
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Systematic validation and atomic force microscopy of non-covalent short oligonucleotide barcode microarrays. [PDF]
PLoS ONE, 2008 BACKGROUND: Molecular barcode arrays provide a powerful means to analyze cellular phenotypes in parallel through detection of short (20-60 base) unique sequence tags, or "barcodes", associated with each strain or clone in a collection.
Michael A Cook +6 more
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Design and analysis of mismatch probes for long oligonucleotide microarrays
BMC Genomics, 2008 Background Nonspecific hybridization is currently a major concern with microarray technology. One of most effective approaches to estimating nonspecific hybridizations in oligonucleotide microarrays is the utilization of mismatch probes; however, this ...
Van Nostrand Joy D +3 more
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ANALYSIS OF COMPLEX MICROBIAL ECOSYSTEMS WITH IN SITU HYBRIDIZATION AND EPIFLUORESCENT MICROSCOPY
Acta Agriculturae Slovenica, 1998 In situ hybridization with fluorescent oligonucleotide probes combined with epifluorescent microscopy was used to detect and localize microorganisms in rumen samples from a black and white Friesian cow.
Katarina TEPŠIČ, G. AVGUŠTIN
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