Results 11 to 20 of about 1,424,575 (357)

Prider: multiplexed primer design using linearly scaling approximation of set coverage

BMC Bioinformatics, 2022
Background Designing oligonucleotide primers and probes is one of the key steps of various laboratory experiments such as multiplexed PCR or digital multiplexed ligation assays.
Niina Smolander, Timothy R. Julian, Manu Tamminen   +2 more
doaj   +1 more source

Systematic validation and atomic force microscopy of non-covalent short oligonucleotide barcode microarrays. [PDF]

PLoS ONE, 2008
BACKGROUND: Molecular barcode arrays provide a powerful means to analyze cellular phenotypes in parallel through detection of short (20-60 base) unique sequence tags, or "barcodes", associated with each strain or clone in a collection.
Michael A Cook, Chi-Kin Chan, Paul Jorgensen, Troy Ketela, Daniel So, Mike Tyers, Chi-Yip Ho   +6 more
doaj   +1 more source

Design and analysis of mismatch probes for long oligonucleotide microarrays

BMC Genomics, 2008
Background Nonspecific hybridization is currently a major concern with microarray technology. One of most effective approaches to estimating nonspecific hybridizations in oligonucleotide microarrays is the utilization of mismatch probes; however, this ...
Van Nostrand Joy D, He Zhili, Deng Ye, Zhou Jizhong   +3 more
doaj   +1 more source

ANALYSIS OF COMPLEX MICROBIAL ECOSYSTEMS WITH IN SITU HYBRIDIZATION AND EPIFLUORESCENT MICROSCOPY

Acta Agriculturae Slovenica, 1998
In situ hybridization with fluorescent oligonucleotide probes combined with epifluorescent microscopy was used to detect and localize microorganisms in rumen samples from a black and white Friesian cow.
Katarina TEPŠIČ, G. AVGUŠTIN
doaj   +1 more source

LUEGO: a cost and time saving gel shift procedure

BioTechniques, 2011
We developed a new approach to prepare DNA probes for electrophoretic mobility gel shift assays that presents a number of advantages compared with the classical approach.
Nicolas Jullien, Jean-Paul Herman
doaj   +1 more source

OligoMiner provides a rapid, flexible environment for the design of genome-scale oligonucleotide in situ hybridization probes

Proceedings of the National Academy of Sciences of the United States of America, 2018
Significance FISH enables researchers to visualize the subcellular distribution of RNA and DNA molecules in individual cells. The recent development of FISH methods employing probes composed of synthetic DNA oligonucleotides (oligos) allows researchers ...
Brian J. Beliveau, Jocelyn Y. Kishi, G. Nir, Hiroshi M. Sasaki, Sinem K. Saka, S. Nguyen, Chao-ting Wu, P. Yin   +7 more
semanticscholar   +1 more source

ROSO: optimizing oligonucleotide probes for microarrays [PDF]

Bioinformatics, 2004
Abstract Summary: ROSO is software to design optimal oligonucleotide probe sets for microarrays. Selected probes show no significant cross-hybridization, no stable secondary structures and their Tm are chosen to minimize the Tm variability of the probe set. Availability: The program is available on the internet.
Federica Calevro, Hubert Charles, J.M. Fayard, Guillaume Beslon, Nancie Reymond, Laurent Duret   +5 more
openaire   +3 more sources

Combined in vitro transcription and reverse transcription to amplify and label complex synthetic oligonucleotide probe libraries

BioTechniques, 2015
Oligonucleotide microarrays allow the production of complex custom oligonucleotide libraries for nucleic acid detection–based applications such as fluorescence in situ hybridization (FISH). We have developed a PCR-free method to make single-stranded DNA (
Yusuf Murgha, Brian Beliveau, Kassandra Semrau, Donald Schwartz, Chao-ting Wu, Erdogan Gulari, Jean-Marie Rouillard   +6 more
doaj   +1 more source

Single-Labeled Oligonucleotides Showing Fluorescence Changes upon Hybridization with Target Nucleic Acids

Molecules, 2018
Sequence-specific detection of nucleic acids has been intensively studied in the field of molecular diagnostics. In particular, the detection and analysis of single-nucleotide polymorphisms (SNPs) is crucial for the identification of disease-causing ...
Gil Tae Hwang
doaj   +1 more source

Spreadsheet Software for Thermodynamic Melting Point Prediction of Oligonucleotide Hybridization with and without Mismatches

BioTechniques, 1999
The use of thermodynamic parameters for the calculation of oligonucleotide duplex stability provides the best estimates of oligonucleotide melting temperatures (Tm). Such estimates can be used for evidence-based design of molecular biological experiments
Ekkehard Schütz, Nicolas von Ahsen
doaj   +1 more source