Results 11 to 20 of about 148,528 (314)

ROSO: optimizing oligonucleotide probes for microarrays [PDF]

Bioinformatics, 2004
Abstract Summary: ROSO is software to design optimal oligonucleotide probe sets for microarrays. Selected probes show no significant cross-hybridization, no stable secondary structures and their Tm are chosen to minimize the Tm variability of the probe set. Availability: The program is available on the internet.
Nancie, Reymond, Hubert, Charles, Laurent, Duret, Federica, Calevro, Guillaume, Beslon, Jean-Michel, Fayard   +5 more
openaire   +4 more sources

Hybridization Analysis Using Oligonucleotide Probe Arrays

, 2007
This chapter describes methodology for the labeling, hybridization, and detection of amplicon target DNA to arrays of oligonucleotide probes attached to plastic substrates. A systematic approach to target discrimination based on both hybridization and wash stringency is provided.
Robert S, Matson, Jang B, Rampal
openaire   +3 more sources

LUEGO: a cost and time saving gel shift procedure

BioTechniques, 2011
We developed a new approach to prepare DNA probes for electrophoretic mobility gel shift assays that presents a number of advantages compared with the classical approach.
Nicolas Jullien, Jean-Paul Herman
doaj   +1 more source

Combined in vitro transcription and reverse transcription to amplify and label complex synthetic oligonucleotide probe libraries

BioTechniques, 2015
Oligonucleotide microarrays allow the production of complex custom oligonucleotide libraries for nucleic acid detection–based applications such as fluorescence in situ hybridization (FISH). We have developed a PCR-free method to make single-stranded DNA (
Yusuf Murgha, Brian Beliveau, Kassandra Semrau, Donald Schwartz, Chao-ting Wu, Erdogan Gulari, Jean-Marie Rouillard   +6 more
doaj   +1 more source

Single-Labeled Oligonucleotides Showing Fluorescence Changes upon Hybridization with Target Nucleic Acids

Molecules, 2018
Sequence-specific detection of nucleic acids has been intensively studied in the field of molecular diagnostics. In particular, the detection and analysis of single-nucleotide polymorphisms (SNPs) is crucial for the identification of disease-causing ...
Gil Tae Hwang
doaj   +1 more source

Fabrication of DNA Microarrays Using Unmodified Oligonucleotide Probes

BioTechniques, 2001
Microarrays printed on glass slides are often constructed by covalently linking oligonucleotide probes to a derivatized surface. These procedures typically require relatively expensive amine- or thiol-modified oligonucleotide probes that add considerable
Douglas Ruben Call, Darrell P. Chandler, Fred Brockman   +2 more
doaj   +1 more source

Spreadsheet Software for Thermodynamic Melting Point Prediction of Oligonucleotide Hybridization with and without Mismatches

BioTechniques, 1999
The use of thermodynamic parameters for the calculation of oligonucleotide duplex stability provides the best estimates of oligonucleotide melting temperatures (Tm). Such estimates can be used for evidence-based design of molecular biological experiments
Ekkehard Schütz, Nicolas von Ahsen
doaj   +1 more source

Oligonucleotides and ND-FISH Displaying Different Arrangements of Tandem Repeats and Identification of Dasypyrum villosum Chromosomes in Wheat Backgrounds

Molecules, 2017
Oligonucleotide probes and the non-denaturing fluorescence in situ hybridization (ND-FISH) technique are widely used to analyze plant chromosomes because they are convenient tools. New oligonucleotide probes, Oligo-Ku, Oligo-3B117.1, Oligo-3B117.2, Oligo-
Zhiqiang Xiao, Shuyao Tang, Ling Qiu, Zongxiang Tang, Shulan Fu   +4 more
doaj   +1 more source

On-chip hybridization kinetics for optimization of gene expression experiments

BioTechniques, 2008
DNA microarray technology is a powerful tool for getting an overview of gene expression in biological samples. Although the successful use of microarray-based expression analysis was demonstrated in a number of applications, the main problem with this ...
Elena Khomyakova, Mikheil A. Livshits, Marie-Caroline Steinhauser, Luce Dauphinot, Sylvia Cohen-Kaminsky, Jean Rossier, Françoise Soussaline, Marie-Claude Potier   +7 more
doaj   +1 more source

Oligonucleotide probes for mutants streptococci

Molecular and Cellular Probes, 1994
Oligodeoxyribonucleotide probes complementary to hypervariable regions of the 16S ribosomal RNA were designed for specificity toward Streptococcus mutans and Streptococcus sobrinus. The probes were tested for specificity and sensitivity by hybridization with nucleic acid from over 100 mutans and non-mutans oral streptococci and other common ...
G A, Cangelosi, J M, Iversen, Y, Zuo, T K, Oswald, R J, Lamont   +4 more
openaire   +2 more sources