Results 221 to 230 of about 148,581 (265)

Oligonucleotide probe design — a new approach

Nature, 1994
The use of oligonucleotides for gene amplification, as diagnostic probes or antisense-based drugs can be optimized by minimizing the possibility of nonspecific hybridization.
M, Mitsuhashi, A, Cooper, M, Ogura, T, Shinagawa, K, Yano, T, Hosokawa   +5 more
openaire   +2 more sources

Oligonucleotide probes for DNA fingerprinting in dogs

Journal of Animal Breeding and Genetics, 1994
SummaryTen different oligonucleotide probes were evaluated for DNA fingerprinting in dogs. Seven probes are able to detect polymorphic bands. Probes (GT)8, (GTG)5 and (GGAT)4 are most informative for individual identification. The probabilities that two individuals from different breeds have the same DNA fingerprint pattern are 1.7 × 10−7, 5.5 × 10−8 ...
M, Jung, K, Wilke, H, Geldermann
openaire   +2 more sources

Probe Design Strategies for Oligonucleotide Microarrays

2016
Oligonucleotide microarrays have been widely used for gene detection and/or quantification of gene expression in various samples ranging from a single organism to a complex microbial assemblage. The success of a microarray experiment, however, strongly relies on the quality of designed probes.
Parisot, Nicolas, Peyretaillade, Eric, Dugat-Bony, Eric, Denonfoux, Jérémie, Mahul, Antoine, Peyret, Pierre   +5 more
openaire   +4 more sources

Oligonucleotide probes for DNA fingerprinting in horses

Journal of Animal Breeding and Genetics, 1993
Summary10 different oligonucleotide probes were evaluated for DNA fingerprinting in horses. Five probes were able to detect polymorphic bands. The probes (GT)8, (GTG)5 and (GGAT)4 are most informative for individual identification and were used to analyze a population of Hannoveranian horses.
K, Wilke, M, Weimann, M, Jung, H, Geldermann   +3 more
openaire   +2 more sources

Oligonucleotide probes functionalization of nanogap electrodes

ELECTROPHORESIS, 2017
AbstractNanogap electrodes have attracted a lot of consideration as promising platform for molecular electronic and biomolecules detection. This is mainly for their higher aspect ratio, and because their electrical properties are easily accessed by current‐voltage measurements. Nevertheless, application of standard current‐voltages measurements used to
Rosa Letizia Zaffino, Mònica Mir, Josep Samitier   +2 more
openaire   +2 more sources

Triplex Tethered Oligonucleotide Probes

Journal of the American Chemical Society, 1996
RNA molecules are believed to possess highly complex structures containing singleand double-stranded regions as well as loops, bulges, and pseudoknots.1 This structural complexity severely complicates efforts to design structureand/or sequenceselective RNA-binding molecules.2,3 Recently we described a family of molecules for the sequenceand structure ...
Arikha C. Moses, Alanna Schepartz
openaire   +1 more source

Microarray oligonucleotide probes.

Methods in enzymology, 2006
Oligonucleotide probes are increasingly the method of choice for many modern DNA microarray applications. They provide higher target specificity, probe selection gives improved experimental control of hybridization properties, and targeting of specific gene subsequences allows better discrimination of highly similar targets such as splice variants or ...
David P, Kreil, Roslin R, Russell, Steven, Russell   +2 more
openaire   +1 more source

[Oligonucleotide probes for the detection of Thermoanaerobacter].

Mikrobiologiia, 2004
Based on the analysis of 16S rRNA nucleotide sequences, oligonucleotide probes were designed for the detection and identification of representatives of the genus Thermoanaerobacter. To increase the specificity level of detection, the genus Thermoanaerobacter was divided into three groups.
I V, Subbotina, N A, Chernykh, T G, Sokolova, I V, Kublanov, E A, Bonch-Osmolovskaia, A V, Lebedinskiĭ   +5 more
openaire   +2 more sources

Application of rRNA-targeted oligonucleotide probes in biotechnology

Applied Microbiology and Biotechnology, 2001
Ribosomal RNA-targeted oligonucleotide probes have become valuable tools for the detection of microorganisms involved in important biotechnological processes. Microorganisms which are of major importance for processes such as wastewater treatment, microbial leaching or methane production can be detected and quantified in situ within a complex microbial
A, Lipski, U, Friedrich, K, Altendorf
openaire   +2 more sources

Choline acetyltransferase expression studied with an oligonucleotide probe

Cellular and Molecular Neurobiology, 1992
1. The localization of choline acetyltransferase messenger RNA has been studied using a digoxigenin-tailed complementary oligodeoxynucleotide probe for in situ hybridization. 2. Putative cholinergic cells of the rat and ferret spinal cord and the ferret retina were labeled. 3. This technique affords superior resolution compared to radioactively labeled
R E, Kennedy, J B, Hutchins
openaire   +2 more sources