Results 21 to 30 of about 1,424,575 (357)
Oligonucleotide probes for mutants streptococci
Molecular and Cellular Probes, 1994 Oligodeoxyribonucleotide probes complementary to hypervariable regions of the 16S ribosomal RNA were designed for specificity toward Streptococcus mutans and Streptococcus sobrinus. The probes were tested for specificity and sensitivity by hybridization with nucleic acid from over 100 mutans and non-mutans oral streptococci and other common ...
Richard J. Lamont +4 more
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Molecules, 2017 Oligonucleotide probes and the non-denaturing fluorescence in situ hybridization (ND-FISH) technique are widely used to analyze plant chromosomes because they are convenient tools. New oligonucleotide probes, Oligo-Ku, Oligo-3B117.1, Oligo-3B117.2, Oligo-
Zhiqiang Xiao +4 more
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Protein detection using oligonucleotide probes [PDF]
Nucleic Acids Symposium Series, 2009 We developed a new nucleic acid-based fluorescence probe for protein detection. The method is based on the scission of an aptamer into two probes, which are then attached with a chemically reactive fluorogenic compound. The protein-dependent association of the two probes accelerates a chemical reaction and indicates the presence of the target protein ...
Satoshi Tsuneda +4 more
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On-chip hybridization kinetics for optimization of gene expression experiments
BioTechniques, 2008 DNA microarray technology is a powerful tool for getting an overview of gene expression in biological samples. Although the successful use of microarray-based expression analysis was demonstrated in a number of applications, the main problem with this ...
Elena Khomyakova +7 more
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Development of a microfluidic biosensor for the diagnostics and typing of Mycobacterium Tuberculosis [PDF]
Клиническая практика, 2021 Background. Despite on the general trend towards decreasing the incidence of newly diagnosed active forms of tuberculosis, the situation with spreading of this disease in Russian Federation remains extremely tense.
Tatiana V. Mitko +7 more
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BioTechniques, 2006 A simple enzymatic labeling procedure is described to determine spot quality in oligonucleotide microarrays. By using fluorescently labeled dideoxynucleotides or ribonucleotides as substrate for terminal deoxynucleotidyl transferase (TdT), a single ...
Cesar E. Guerra
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A strategy for oligonucleotide microarray probe reduction [PDF]
Genome Biology, 2002 One of the factors limiting the number of genes that can be analyzed on high-density oligonucleotide arrays is that each transcript is probed by multiple oligonucleotide probes. To reduce the number of probes required for each gene, a systematic approach to choosing the most representative probes is needed. A method is presented for reducing the number
Todd R. Golub +3 more
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Sensors, 2010 The CombiMatrix microarray with 12,544 electrodes supports in situ electrochemical synthesis of user-defined DNA probes. As an alternative, we immobilized commercially synthesized DNA probes on individual electrodes coated with electropolymerized ...
David Danley +5 more
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Specific and Nonspecific Hybridization of Oligonucleotide Probes on Microarrays [PDF]
Biophysical Journal, 2005 Gene expression analysis by means of microarrays is based on the sequence specific binding of mRNA to DNA oligonucleotide probes and its measurement using fluorescent labels. The binding of RNA fragments involving other sequences than the intended target is problematic because it adds a "chemical background" to the signal, which is not related to the ...
Stephan Preibisch, Hans Binder
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BioTechniques, 1998 The FastTag™ Nucleic Acid Labeling System couples haptens, fluorochromes or affinity ligands to any nucleic acid by attaching a universal, photo- or heat-activatable moiety to which any sulfhydryl-reactive compound can be linked.
Steven G. Daniel +3 more
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