Results 21 to 30 of about 148,528 (314)

Single-Tube Genotyping without Oligonucleotide Probes [PDF]

Genome Research, 1999
We report the development of a self-contained (homogeneous), single-tube assay for the genotyping of single-nucleotide polymorphisms (SNPs), which does not rely on fluorescent oligonucleotide probes. The method, which we call Tm-shift genotyping, combines allele-specific PCR with the discrimination between amplification products by their melting ...
S, Germer, R, Higuchi
openaire   +2 more sources

Development of a microfluidic biosensor for the diagnostics and typing of Mycobacterium Tuberculosis [PDF]

Клиническая практика, 2021
Background. Despite on the general trend towards decreasing the incidence of newly diagnosed active forms of tuberculosis, the situation with spreading of this disease in Russian Federation remains extremely tense.
Tatiana V. Mitko, Ruslan I. Shakurov, Fedor V. Shirshikov, Sizova V. Svetlana, Elena V. Alieva, Valery N. Konopsky, Dmitry V. Basmanov, Julia A. Bespyatykh   +7 more
doaj   +1 more source

Analysis of oligonucleotide microarrays by 3′ end labeling using fluorescent nucleotides and terminal transferase

BioTechniques, 2006
A simple enzymatic labeling procedure is described to determine spot quality in oligonucleotide microarrays. By using fluorescently labeled dideoxynucleotides or ribonucleotides as substrate for terminal deoxynucleotidyl transferase (TdT), a single ...
Cesar E. Guerra
doaj   +1 more source

Use of a Multiplexed CMOS Microarray to Optimize and Compare Oligonucleotide Binding to DNA Probes Synthesized or Immobilized on Individual Electrodes

Sensors, 2010
The CombiMatrix microarray with 12,544 electrodes supports in situ electrochemical synthesis of user-defined DNA probes. As an alternative, we immobilized commercially synthesized DNA probes on individual electrodes coated with electropolymerized ...
David Danley, Wanda Lyon, John Cooper, Jodi Wilmoth, Nina Yazvenko, Karl Maurer   +5 more
doaj   +1 more source

FastTag™ Nucleic Acid Labeling System: A Versatile Method for Incorporating Haptens, Fluorochromes and Affinity Ligands into DNA, RNA and Oligonucleotides

BioTechniques, 1998
The FastTag™ Nucleic Acid Labeling System couples haptens, fluorochromes or affinity ligands to any nucleic acid by attaching a universal, photo- or heat-activatable moiety to which any sulfhydryl-reactive compound can be linked.
Steven G. Daniel, Mark E. Westling, Michele S. Moss, Brian D. Kanagy   +3 more
doaj   +1 more source

Development, characterization and experimental validation of a cultivated sunflower (Helianthus annuus L.) gene expression oligonucleotide microarray. [PDF]

PLoS ONE, 2012
Oligonucleotide-based microarrays with accurate gene coverage represent a key strategy for transcriptional studies in orphan species such as sunflower, H. annuus L., which lacks full genome sequences.
Paula Fernandez, Marcelo Soria, David Blesa, Julio DiRienzo, Sebastian Moschen, Maximo Rivarola, Bernardo Jose Clavijo, Sergio Gonzalez, Lucila Peluffo, Dario Príncipi, Guillermo Dosio, Luis Aguirrezabal, Francisco García-García, Ana Conesa, Esteban Hopp, Joaquín Dopazo, Ruth Amelia Heinz, Norma Paniego   +17 more
doaj   +1 more source

ProbeMaker: an extensible framework for design of sets of oligonucleotide probes

BMC Bioinformatics, 2005
Background Procedures for genetic analyses based on oligonucleotide probes are powerful tools that can allow highly parallel investigations of genetic material.
Nilsson Mats, Stenberg Johan, Landegren Ulf   +2 more
doaj   +1 more source

Dynamic probe selection for studying microbial transcriptome with high-density genomic tiling microarrays

BMC Bioinformatics, 2010
Background Current commercial high-density oligonucleotide microarrays can hold millions of probe spots on a single microscopic glass slide and are ideal for studying the transcriptome of microbial genomes using a tiling probe design.
Chen Tsute, Høvik Hedda
doaj   +1 more source

Generation of Amplifiable Genome-Specific Oligonucleotide Probes and Libraries

BioTechniques, 2002
Here we describe a process for the generation of oligonucleotide libraries representative of a given nucleic acid. Starting from a random pool of DNA oligonucleotides, the technique selects only those that hybridize to the nucleic acid template.
Ivan Brukner, Guy A. Tremblay, Bruno Paquin   +2 more
doaj   +1 more source

Oligonucleotide microarray analysis of aminoallyl-labeled cDNA targets from linear RNA amplification

BioTechniques, 2004
Single-stranded long oligonucleotide-based (50- to 70-mer) microarrays offer several advantages over conventional cDNA microarrays. These include the easy preparation of the probes, low cost of array production, and low cross-contamination during probe ...
Pal Kaposi-Novak, Ju-Seog Lee, Arsen Mikaelyan, Vyomesh Patel, Snorri S. Thorgeirsson   +4 more
doaj   +1 more source