Results 51 to 60 of about 1,544,426 (289)

Calcium signaling in oocyte quality and functionality and its application

Frontiers in Endocrinology
Calcium (Ca2+) is a second messenger for many signal pathways, and changes in intracellular Ca2+ concentration ([Ca2+]i) are an important signaling mechanism in the oocyte maturation, activation, fertilization, function regulation of granulosa and ...
Chen Chen, Zefan Huang, Shijue Dong, Mengqian Ding, Jinran Li, Miaomiao Wang, Xuhui Zeng, Xiaoning Zhang, Xiaoli Sun   +8 more
doaj   +1 more source

Oocyte activation and phospholipase C zeta (PLCζ): diagnostic and therapeutic implications for assisted reproductive technology

Cell Communication and Signaling, 2012
Infertility affects one in seven couples globally and has recently been classified as a disease by the World Health Organisation (WHO). While in-vitro fertilisation (IVF) offers effective treatment for many infertile couples, cases exhibiting severe male
Ramadan Walaa M, Kashir Junaid, Jones Celine, Coward Kevin   +3 more
doaj   +1 more source

Comparison of Human Oocyte Activation Between Round-Headed Sperm Injection Followed by Calcium Ionophore Treatment and Normal Sperm Injection in a Patient With Globozoospermia

Frontiers in Endocrinology, 2020
Fertilization failure is common in patients with round-headed sperm, a form of globozoospermia. Artificial oocyte activation is able to assist oocyte fertilization after sperm injection in these patients.
Xiangli Niu, Qiuyan Ruan, Craig A. Witz, Weihua Wang   +3 more
doaj   +1 more source

Paternal Sperm Gnas‐ICR Epigenetic Programming Contributes to PPP‐Like Phenotypes in Female Offspring

Advanced Science, EarlyView.
A paternal glucocorticoid–sperm Gnas‐ICR axis is linked to ovarian endocrine programming in female offspring. Paternal preconception caffeine exposure elevates corticosterone, promotes sperm Gnas‐ICR hypermethylation, and is associated with ovarian Gnas upregulation, cAMP/PKA/CREB–StAR activation, enhanced estradiol synthesis, and PPP‐like phenotypes ...
Jing Huang, Lu Chen, Tiancheng Wu, Yating Li, Hui Wang   +4 more
wiley   +1 more source

Effects of protein kinase C on parthenogenetic activation of pig oocytes using calcium ionophore or nitric oxide-donor

Czech Journal of Animal Science, 2007
Porcine oocytes matured in vitro were activated for parthenogenetic development using either calcium ionophore (50μM for 10 min) or nitric oxide donor SNAP (2mM for 23.5 hours).
J. Petr, E. Chmelíková, A. Dörflerová, M. Ješeta, Z. Kuthanová   +4 more
doaj   +1 more source

How to improve the clinical outcome of round spermatid injection (ROSI) into the oocyte: Correction of epigenetic abnormalities

Reproductive Medicine and Biology, 2023
Background First successful human round spermatid injection (ROSI) was conducted by Tesarik et al. in 1996 for the sole treatment of nonobstructive azoospermic men whose most advanced spermatogenic cells were elongating round spermatids.
Atsushi Tanaka, Seiji Watanabe
doaj   +1 more source

Spatially Organized Human Ovarian Spheroids Instruct Endometrial Morphogenesis

Advanced Science, EarlyView.
A spatially organized human follicle‐like spheroid (SPHEGaT) composed of granulosa cells (GCs) and stromal‐derived theca‐like cells (TLCs) establishes sustained steroidogenesis in vitro. When co‐cultured with human endometrial organoids (EMOs), estradiol (E2) and progesterone (P4) exchange induces secretory/progesterone‐responsive differentiation ...
Maria João Sousa, Silke De Vriendt, Lixian Liu, Thalles Fernando Rocha Ruiz, Hugo Vankelecom, Christiani Andrade Amorim   +5 more
wiley   +1 more source

Fertilization and activation currents in bovine oocytes [PDF]

Reproduction, 2002
Abstract One of the first events that occurs at fertilization is a transient modification of the electrical properties of the oocyte plasma membrane. The whole-cell voltage clamp technique was used to demonstrate an outward ion current and a hyperpolarization of the plasma membrane after fertilization in bovine oocytes.
TOSTI E., BONI, Raffaele, CUOMO A.
openaire   +2 more sources

Deciphering Anti‐Cancer Drug Efficacy Through Nanomechanical Vibrations in Living Gastric Cancer Organoids

Advanced Science, EarlyView.
This study developed an efficacy assessment platform that integrates patient‐derived gastric cancer organoids, atomic force microscopy (AFM)‐based nanomechanical vibration detection, deep learning analysis, and organoid mechanical modeling. It detects picomolar drug effects within 0.1 s signal, achieves 97% classification accuracy, and offers non ...
Ting Zhang, Qiubo Chen, Shihai Lan, Liang Zhu, Shuaiyu Liu, Yan Xia, Yalu Zhang, Hanhui Yao, Yongman Liu, Shangquan Wu, Qingchuan Zhang   +10 more
wiley   +1 more source

Participação do fator de crescimento fibroblástico 18 (FGF18) na maturação nuclear oocitária, expansão das células do cumulus e produção in vitro de embriões bovinos [PDF]

, 2015
Dissertação (mestrado) - Universidade Federal de Santa Catarina, Centro de Ciências Biológicas, Programa de Pós-Graduação em Biologia Celular e do Desenvolvimento, Florianópolis, 2015.Este trabalho propôs estudar a ação do Fator de Crescimento ...
Silva, Yago Pinto da
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