Results 61 to 70 of about 162,248 (282)
Extensive translation of small Open Reading Frames revealed by Poly-Ribo-Seq
eLife, 2014 Thousands of small Open Reading Frames (smORFs) with the potential to encode small peptides of fewer than 100 amino acids exist in our genomes. However, the number of smORFs actually translated, and their molecular and functional roles are still unclear.
Julie L Aspden +6 more
doaj +1 more source
Maintenance of the correct open reading frame by the ribosome [PDF]
The EMBO Reports, 2003 During translation, a string of non-overlapping triplet codons in messenger RNA is decoded into protein. The ability of a ribosome to decode mRNA without shifting between reading frames is a strict requirement for accurate protein biosynthesis. Despite enormous progress in understanding the mechanism of transfer RNA selection, the mechanism by which ...
Thomas M, Hansen +4 more
openaire +2 more sources
Genomic Signals of Reoriented ORFs
EURASIP Journal on Advances in Signal Processing, 2004 Complex representation of nucleotides is used to convert DNA sequences into complex digital genomic signals. The analysis of the cumulated phase and unwrapped phase of DNA genomic signals reveals large-scale features of eukaryote and prokaryote ...
Paul Dan Cristea
doaj +1 more source
Mini-Exon Epitope Tagging for Analysis of the Protein Coding Potential of Genomic Sequence
BioTechniques, 1997 A novel approach to gene discovery and analysis is described. A small exon flanked by consensus 3′ and 5′ splice sites was synthesized. The exon contains open reading frames encoding 43 amino acid peptides.
Desmond J. Smith
doaj +1 more source
Viruses, 2023 Bacteriophage genomes represent an enormous level of genetic diversity and provide considerable potential to acquire new insights about viral genome evolution. In this study, the genome sequences of sixteen Bacillus-infecting bacteriophages were explored
Ahmed Ismail +6 more
doaj +1 more source
uORF-Tools-Workflow for the determination of translation-regulatory upstream open reading frames.
PLoS ONE, 2019 Ribosome profiling (ribo-seq) provides a means to analyze active translation by determining ribosome occupancy in a transcriptome-wide manner. The vast majority of ribosome protected fragments (RPFs) resides within the protein-coding sequence of mRNAs ...
Anica Scholz +7 more
doaj +1 more source
FEBS Letters, EarlyView.We present robust protocols for the preparation of supported lipid bilayers (SLBs) incorporating either Salmonella smooth LPS or outer membrane vesicles (OMVs). We use a combination of quartz crystal microbalance with dissipation (QCM‐D) and fluorescence microscopy to both characterize the SLBs of various compositions and to probe their interactions ...
Hudson P. Pace +6 more
wiley +1 more source
Enrichment of open reading frames presented on bacteriophage M13 using Hyperphage
BioTechniques, 2006 The enrichment of open reading frames (ORFs) from large gene libraries and the presentation of the corresponding polypeptides on filamentous phage M13 (phage display) is frequently used to identify binding partners of unknown ORFs.
Michael Hust +6 more
doaj +1 more source
Disrupting upstream translation in mRNAs is associated with human disease
Nature Communications, 2021 The significance of translated upstream open reading frames is not well known. Here, the authors investigate genetic variants in these regions, finding that they are under high evolutionary constraint and may contribute to disease.
David S. M. Lee +7 more
doaj +1 more source
Investigating transcription factor dynamics in health and disease using FRAP
FEBS Letters, EarlyView.FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj +3 more
wiley +1 more source