Results 61 to 70 of about 3,449,089 (306)

Investigating transcription factor dynamics in health and disease using FRAP

FEBS Letters, EarlyView.
FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj, Carolina Vazquez Garzon, Jose Joaquin Velasco, Janine N. Post   +3 more
wiley   +1 more source

Marker-Free Tracking for Motion Artifact Compensation and Deformation Measurements in Optical Mapping Videos of Contracting Hearts

Frontiers in Physiology, 2018
Optical mapping is a high-resolution fluorescence imaging technique, which provides highly detailed visualizations of the electrophysiological wave phenomena, which trigger the beating of the heart.
Jan Christoph, Jan Christoph, Jan Christoph, Stefan Luther, Stefan Luther, Stefan Luther, Stefan Luther   +6 more
doaj   +1 more source

Neonatal Deletion of Hand1 and Hand2 within Murine Cardiac Conduction System Reveals a Novel Role for HAND2 in Rhythm Homeostasis

Journal of Cardiovascular Development and Disease, 2022
The cardiac conduction system, a network of specialized cells, is required for the functioning of the heart. The basic helix loop helix factors Hand1 and Hand2 are required for cardiac morphogenesis and have been implicated in cardiac conduction system ...
Rajani M. George, Shuai Guo, Beth A. Firulli, Michael Rubart, Anthony B. Firulli   +4 more
doaj   +1 more source

Conserved binding mode but diverse interfaces of MreC‐PBP2 interactions

FEBS Letters, EarlyView.
The crystal structure of abMreC reveals a conserved two β‐barrel architecture and provides structural insights into its role within the bacterial elongasome. The abMreC–abPBP2 complex model identifies the molecular basis of MreC‐mediated PBP2 recognition, contributing to the regulation of peptidoglycan synthesis.
Hyunseok Jang, Hyo Been Jin, Chang Min Kim, So Yeon Lee, Hyun Ho Park   +4 more
wiley   +1 more source

Simultaneous Quantification of Spatially Discordant Alternans in Voltage and Intracellular Calcium in Langendorff-Perfused Rabbit Hearts and Inconsistencies with Models of Cardiac Action Potentials and Ca Transients

Frontiers in Physiology, 2017
Rationale: Discordant alternans, a phenomenon in which the action potential duration (APDs) and/or intracellular calcium transient durations (CaDs) in different spatial regions of cardiac tissue are out of phase, present a dynamical instability for ...
Ilija Uzelac, Yanyan C. Ji, Daniel Hornung, Johannes Schröder-Scheteling, Stefan Luther, Richard A. Gray, Elizabeth M. Cherry, Flavio H. Fenton   +7 more
doaj   +1 more source

Synergistic perspectives—How single‐molecule biophysics complement biochemical understanding

FEBS Letters, EarlyView.
In this review, we discuss how ensemble biochemistry and single‐molecule approaches are complementary, outline commonly used single‐molecule techniques, and illustrate their relevance through two representative case studies: chromatin organization by SMC complexes and pathway choice during DNA double‐strand break repair.
Sara De Bragança, Eline L. Jansen, Jorine M. Eeftens   +2 more
wiley   +1 more source

Cardiac Optogenetics and Optical Mapping – Overcoming Spectral Congestion in All-Optical Cardiac Electrophysiology

Frontiers in Physiology, 2019
Optogenetic control of the heart is an emergent technology that offers unparalleled spatio-temporal control of cardiac dynamics via light-sensitive ion pumps and channels (opsins). This fast-evolving technique holds broad scope in both clinical and basic
Christopher O’Shea, Christopher O’Shea, Christopher O’Shea, Andrew P. Holmes, Andrew P. Holmes, James Winter, Joao Correia, Xianhong Ou, Ruirui Dong, Shicheng He, Paulus Kirchhof, Paulus Kirchhof, Larissa Fabritz, Larissa Fabritz, Kashif Rajpoot, Davor Pavlovic   +15 more
doaj   +1 more source

Arrhythmic Risk Assessment of Hypokalaemia Using Human Pluripotent Stem Cell-Derived Cardiac Anisotropic Sheets

Frontiers in Cell and Developmental Biology, 2021
Introduction: Hypokalaemia, defined as an extracellular concentration of K+ below 3.5 mM, can cause cardiac arrhythmias by triggered or re-entrant mechanisms.
Bimal Gurung, Gary Tse, Gary Tse, Gary Tse, Wendy Keung, Wendy Keung, Ronald A. Li, Wing Tak Wong, Wing Tak Wong, Wing Tak Wong   +9 more
doaj   +1 more source

Functional comparison of EncB and EncC cargo proteins in iron storage within the Myxococcus xanthus encapsulin

FEBS Letters, EarlyView.
Encapsulins are protein nanocompartments that play an important role in iron storage. In the Myxococcus xanthus encapsulin system, two cargo proteins called EncB and EncC contribute to iron mineralization. Here, we show that EncB and EncC generate iron‐containing minerals with distinct chemical compositions, suggesting that the composition of stored ...
Harry B. McDowell, Thomas Walther, Egbert Hoiczyk   +2 more
wiley   +1 more source

Efficient Image Registration for Underwater Optical Mapping Using Geometric Invariants

Journal of Marine Science and Engineering, 2019
Image registration is one of the most fundamental and widely used tools in optical mapping applications. It is mostly achieved by extracting and matching salient points (features) described by vectors (feature descriptors) from images. While matching the
Armagan Elibol, Nak Young Chong
doaj   +1 more source