Results 61 to 70 of about 152,381 (261)
Frontiers in Physiology, 2019 Optogenetic control of the heart is an emergent technology that offers unparalleled spatio-temporal control of cardiac dynamics via light-sensitive ion pumps and channels (opsins). This fast-evolving technique holds broad scope in both clinical and basic
Christopher O’Shea +15 more
doaj +1 more source
Optical mapping of electromechanics in intact organs [PDF]
Experimental Biology and Medicine, 2019 Optical mapping has become a widely used and important method in cardiac electrophysiology. The method typically uses voltage-sensitive fluorescent dyes and high-speed cameras to image propagation of electrical waves. However, signals are highly susceptible to artifact caused by motion of the target organ.
Haley W Nesmith +2 more
openaire +2 more sources
Design and analysis strategies for robust microbiome ageing research
FEBS Letters, EarlyView.The gut microbiome changes with age and associates with age‐related morbidity and mortality, establishing it as a potential biomarker and intervention target for ageing. Realising this potential requires methodological rigour, yet distinguishing biological signals from methodological artefacts remains challenging across cohorts. This review provides an
Mark Olenik +5 more
wiley +1 more source
Efficient Image Registration for Underwater Optical Mapping Using Geometric Invariants
Journal of Marine Science and Engineering, 2019 Image registration is one of the most fundamental and widely used tools in optical mapping applications. It is mostly achieved by extracting and matching salient points (features) described by vectors (feature descriptors) from images. While matching the
Armagan Elibol, Nak Young Chong
doaj +1 more source
Optical mapping at increased illumination intensities [PDF]
Journal of Biomedical Optics, 2012 Voltage-sensitive fluorescent dyes have become a major tool in cardiac and neuro-electrophysiology. Achieving high signal-to-noise ratios requires increased illumination intensities, which may cause photobleaching and phototoxicity. The optimal range of illumination intensities varies for different dyes and must be evaluated individually.
Giedrius, Kanaporis +7 more
openaire +2 more sources
Investigating transcription factor dynamics in health and disease using FRAP
FEBS Letters, EarlyView.FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj +3 more
wiley +1 more source
Synapse-specific inhibitory control of hippocampal feedback inhibitory circuit
Frontiers in Cellular Neuroscience, 2010 Local circuit and long-range GABAergic projections provide powerful inhibitory control over the operation of hippocampal inhibitory circuits, yet little is known about the input- and target-specific organization of interacting inhibitory networks in ...
Simon eChamberland +6 more
doaj +1 more source
Conserved binding mode but diverse interfaces of MreC‐PBP2 interactions
FEBS Letters, EarlyView.The crystal structure of abMreC reveals a conserved two β‐barrel architecture and provides structural insights into its role within the bacterial elongasome. The abMreC–abPBP2 complex model identifies the molecular basis of MreC‐mediated PBP2 recognition, contributing to the regulation of peptidoglycan synthesis.
Hyunseok Jang +4 more
wiley +1 more source
Optical mapping in plant comparative genomics [PDF]
GigaScience, 2015 Optical mapping has been widely used to improve de novo plant genome assemblies, including rice, maize, Medicago, Amborella, tomato and wheat, with more genomes in the pipeline. Optical mapping provides long-range information of the genome and can more easily identify large structural variations.
Tang, Haibao +2 more
openaire +2 more sources
The role of miR‐335‐5p in the redifferentiation of BRAF p.V600E thyroid cancers
Molecular Oncology, EarlyView.The BRAF p.V600E mutation promotes thyroid cancer dedifferentiation and radioiodine resistance. Using a network approach, we identified miR‐335‐5p as a key regulator of BRAF‐mutated thyroid tumors. Restoring miR‐335‐5p increased thyroid‐specific gene expression and iodine uptake in cells and organoids.
Valeria Pecce +11 more
wiley +1 more source