Results 81 to 90 of about 2,111,052 (290)

Improvement of the Optimum pH of Aspergillus niger Xylanase towards an Alkaline pH by Site-Directed Mutagenesis

Journal of Microbiology and Biotechnology, 2015
In an attempt to shift the optimal pH of the xylanase B (XynB) from Aspergillus niger towards alkalinity, target mutation sites were selected by alignment between Aspergillus niger xylanase B and other xylanases that have alkalophilic pH optima that highlight charged residues in the eight-residues-longer loop in the alkalophilic xylanase.
Fei, Li, Jingcong, Xie, Xuesong, Zhang, Linguo, Zhao   +3 more
openaire   +2 more sources

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

The optimum pH values of AR/BDH from different microorganisms.

, 2014
The optimum pH values of AR/BDH from different microorganisms.
Xian Zhang (191440), Teng Bao (532118), Zhiming Rao (465690), Shangtian Yang (532119), Huazhong Li (296644), Zhenghong Xu (412193), Taowei Yang (465689)   +6 more
core   +1 more source

The Analysis of the Ability NaOH Activated Rice Husk Ash Silica to Reduce Cl Ion Levels

Indonesian Journal of Chemical Research
Research has been carried out to analyze the ability of NaOH-activated rice husk Ash Silica to reduce Cl Ion Levels. This study aimed to determine the optimum mass, optimum pH, optimum contact time and apply NaOH-activated rice husk ash silica to reduce
Nurul Widya Febryzha, La Harimu, Wa Ode Mulyana, Ekacahyana Mandasari   +3 more
doaj   +1 more source

Acidic Shift of Optimum pH of Bovine Serum Amine Oxidase upon Immobilization onto Nanostructured Ferric Tannates. [PDF]

Int J Mol Sci, 2022
Rilievo G, Cecconello A, Molinari S, Venerando A, Rutigliano L, Govardhan GT, Kariyawasam DH, Arusei RJ, Zennaro L, Di Paolo ML, Agostinelli E, Vianello F, Magro M.   +12 more
europepmc   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Cloning, purification and comparative characterization of two digestive lysozymes from Musca domestica larvae

Brazilian Journal of Medical and Biological Research, 2008
cDNA coding for two digestive lysozymes (MdL1 and MdL2) of the Musca domestica housefly was cloned and sequenced. MdL2 is a novel minor lysozyme, whereas MdL1 is the major lysozyme thus far purified from M. domestica midgut.
F.C. Cançado, P Chimoy Effio, W.R. Terra, S.R. Marana   +3 more
doaj  

A Novel In Vitro Model for Determining the Optimum pH and Dose Volume of New Liquid Alginate for Infant Reflux Suppression. [PDF]

Drugs R D, 2021
Fisher J, McLaughlin F, Fawkes N, Tipple H, Coyle C, Dettmar PW.   +5 more
europepmc   +1 more source

Aquaporin‐3 and aquaporin‐5 impact the development of pancreatic ductal adenocarcinoma spheroids

FEBS Open Bio, EarlyView.
Schematic representation of the role of aquaporin‐3 (AQP3) and aquaporin‐5 (AQP5) in pancreatic ductal adenocarcinoma (PDAC). Both proteins are upregulated in PDAC and are associated with tumor progression and metastatic potential. Silencing AQP3 or AQP5 in PDAC spheroids results in decreased diameter, area, and overall growth, underscoring their key ...
Catarina Pimpão, Diogo M. Engrácia, Graça Soveral, Filipa Mendes   +3 more
wiley   +1 more source

Giresun Yöresinde Yetişen Fındık Mantarı (Lactarius pyragalus)’ndan Katalaz Enziminin Saflaştırılması Ve Karakterizasyonu

Karadeniz Fen Bilimleri Dergisi, 2018
Katalaz (E.C. 1.11.1.6), kararlı ve güçlü bir yükseltgen olan hidrojen peroksitin su ve moleküler oksijene dönüşümünü katalizleyerek parçalanmasını sağlayan enzimdir.
Bahar BİLGİN SÖKMEN, Ahmet AHISKALI
doaj