Results 11 to 20 of about 587,545 (300)

Identification of ORC1/CDC6-interacting factors in trypanosoma brucei reveals critical features of origin recognition complex architecture [PDF]

, 2012
DNA Replication initiates by formation of a pre-replication complex on sequences termed origins. In eukaryotes, the pre-replication complex is composed of the Origin Recognition Complex (ORC), Cdc6 and the MCM replicative helicase in conjunction with ...
Barry, J.D., Bell, S.D., Burchmore, R., Farr, H., Gadelha, C., Marcello, L., McCulloch, R., Tiengwe, C.   +7 more
core   +7 more sources

An ATR and CHK1 kinase signaling mechanism that limits origin firing during unperturbed DNA replication

Proceedings of the National Academy of Sciences of the United States of America, 2019
Significance The 50,000 origins that replicate the human genome are selected from an excess of licensed origins. Firing licensed origins that would otherwise be passively replicated is a simple mechanism to recover DNA replication between stalled ...
T. Moiseeva, Yandong Yin, Michael J. Calderon, Chenao Qian, Sandy Schamus-Haynes, Norie Sugitani, Hatice U. Osmanbeyoglu, E. Rothenberg, Simon C Watkins, C. Bakkenist   +9 more
semanticscholar   +1 more source

Identification of a Novel 81-kDa Component of the Xenopus Origin Recognition Complex [PDF]

, 1998
The Xenopus origin recognition complex is essential for chromosomal DNA replication in cell-free extracts. We have immunopurified the Xenopus origin recognition complex with anti-Xorc2 antibodies and analyzed its composition and properties.
Carpenter, Phillip B., Dunphy, William G.   +1 more
core   +1 more source

The level of origin firing inversely affects the rate of replication fork progression

Journal of Cell Biology, 2013
Cells with reduced origin firing have an increased rate of replication fork progression, whereas fork progression is slowed in cells with excess origins.
Yuan Zhong, T. Nellimoottil, Jared M. Peace, S. Knott, Sandra K. Villwock, Janis M. Yee, Jeffrey M. Jancuska, Sanket V. Rege, M. Tecklenburg, R. Sclafani, S. Tavaré, O. Aparicio   +11 more
semanticscholar   +1 more source

Mechanism of DNA Recognition at a Viral Replication Origin [PDF]

, 2006
Recognition of the DNA origin by the Epstein-Barr nuclear antigen 1 (EBNA1) protein is the primary event in latentphase genome replication of the Epstein-Barr virus, a model for replication initiation in eukaryotes.
de Prat Gay, Gonzalo, Frappier, Lori, Freire Espeleta, Eleonora, Oddo, Cristian   +3 more
core   +1 more source

Chk1 inhibits replication factory activation but allows dormant origin firing in existing factories [PDF]

, 2010
Replication origins are licensed by loading MCM2-7 hexamers before entry into S phase. However, only ∼10% of licensed origins are normally used in S phase, with the others remaining dormant.
Anglana, Arias, Bartek, Berezney, Blow, Blow, Blow, Bourke, Boutros, Branzei, Chung, Cseresnyes, Dijkwel, Dimitrova, Doksani, Ge, Gilbert, Gillespie, Hiratani, Ibarra, J. Julian Blow, Jackson, Kitamura, Krasinska, Kunnev, Lambert, Leonhardt, Lopes, Maya-Mendoza, Ockey, Pruitt, Sadoni, Santocanale, Santocanale, Shima, Shirahige, Sporbert, Taylor, Tercero, Thomson, Timofeev, Tuduri, Woodward, Xin Quan Ge, Zachos   +44 more
core   +4 more sources

Human RIF1 and protein phosphatase 1 stimulate DNA replication origin licensing but suppress origin activation

EMBO Reports, 2017
The human RIF1 protein controls DNA replication, but the molecular mechanism is largely unknown. Here, we demonstrate that human RIF1 negatively regulates DNA replication by forming a complex with protein phosphatase 1 (PP1) that limits phosphorylation ...
S. Hiraga, T. Ly, J. Garzón, Z. Hořejší, Yoshinobu Ohkubo, Akinori Endo, C. Obuse, S. Boulton, A. Lamond, A. Donaldson   +9 more
semanticscholar   +1 more source

USF binding sequences from the HS4 insulator element impose early replication timing on a vertebrate replicator [PDF]

, 2012
The nuclear genomes of vertebrates show a highly organized program of DNA replication where GC-rich isochores are replicated early in S-phase, while AT-rich isochores are late replicating.
Boggetto, N., Cadoret, J-C., Chilaka, S., Hassan-Zadeh, V., Ma, M., Prioleau, M-N., West, A. G.   +6 more
core   +9 more sources

Nucleosomes influence multiple steps during replication initiation [PDF]

, 2016
Eukaryotic replication origin licensing, activation and timing are influenced by chromatin but a mechanistic understanding is lacking. Using reconstituted nucleosomal DNA replication assays, we assessed the impact of nucleosomes on replication initiation.
Bell, Belsky, Berbenetz, Biswas, Boyle, Clapier, Clapier, Cole, Devbhandari, Dimitrova, Donovan, Eaton, Euskirchen, Flanagan, Flaus, Flores, Foltman, Fragkos, Francis, Frigola, Gros, Gros, Hamiche, Hargreaves, Heller, Huang, Ilves, Kang, Kassabov, Kurat, Langmead, Li, Lipford, Longtine, Lorch, Längst, Lõoke, MacAlpine, MacAlpine, Marahrens, Mizuguchi, Mizuguchi, Monahan, Müller, On, Papamichos-Chronakis, Papamichos-Chronakis, Papamichos-Chronakis, Peace, Poot, Raghuraman, Randell, Rhind, Santocanale, Schnitzler, Shen, Siddiqui, Simpson, Smith, Struhl, Tanaka, Tsukiyama, Ulyanova, Vary, Watanabe, Whitehouse, Wyrick, Xu, Yang, Yeeles, Yeeles, Yen   +71 more
core   +3 more sources

Mutant p53 establishes targetable tumor dependency by promoting unscheduled replication [PDF]

, 2017
Gain-of-function (GOF) p53 mutations are observed frequently in most intractable human cancers and establish dependency for tumor maintenance and progression.
Beckerman, Bronte, Catherine A. Vaughan, Frum, Lin, Luzhna, Olivier, Oren, Rebecca A. Frum, Shilpa Singh, Steven R. Grossman, Sumitra Deb, Swati Palit Deb, Zeman, Zuazua-Villar   +14 more
core   +2 more sources