Results 61 to 70 of about 598,816 (344)

Mechanosensitive Self-Replication Driven by Self-Organization [PDF]

, 2010
Self-replicating molecules are likely to have played an important role in the origin of life, and a small number of fully synthetic self-replicators have already been described.
Belenguer, Ana M.,, Carnall, Jacqui M.A.,, Otto, Sijbren,, Peyralans, Jérôme J.-P.,, Stuart, Marc C.A.,, Waudby, Christopher A.,   +5 more
core   +3 more sources

The elusive determinants of replication origins [PDF]

EMBO reports, 2007
Despite their central role in maintaining genetic integrity during cell division, there is still considerable uncertainty about how DNA sequences are selected to be replication origins in eukaryotes (DePamphilis, 1999; Gilbert, 2001; Machida et al , 2005). The budding yeast Saccharomyces cerevisiae uses a limited set of efficient origins, each of which
Costa, Silvia, Blow, J Julian
openaire   +3 more sources

Organoids in pediatric cancer research

FEBS Letters, EarlyView.
Organoid technology has revolutionized cancer research, yet its application in pediatric oncology remains limited. Recent advances have enabled the development of pediatric tumor organoids, offering new insights into disease biology, treatment response, and interactions with the tumor microenvironment.
Carla Ríos Arceo, Jarno Drost
wiley   +1 more source

Escherichia coli cells evade inducible parE toxin expression by reducing plasmid copy number

Microbiology Spectrum
Plasmids play important roles in microbial ecosystems, serving as carriers of antibiotic resistance and virulence. In the laboratory, they are essential tools for genetic manipulation and recombinant protein expression.
Shengfeng Ruan, Christina R. Bourne
doaj   +1 more source

Kinetic model of DNA replication in eukaryotic organisms

, 2003
We formulate a kinetic model of DNA replication that quantitatively describes recent results on DNA replication in the in vitro system of Xenopus laevis prior to the mid-blastula transition.
Avrami, Avrami, Avrami, Ben-Naim, Bensimon, Berezney, Blow, Blow, Blow, Blow, Blumenthal, Brewer, Buongiorno-Nardelli, Cahn, Cairns, Christian, Coverley, Friedman, Gilbert, Gomez, Hand, Herrick, Herrick, Huberman, Hyrien, Jackson, Jasny, Johnson, Kolmogorov, Laskey, Lu, Lucas, Mahbubani, Marheineke, Michalet, Mills, Pasero, Pierron, Plischke, Press, Raghuraman, Rowles, Sekimoto, Shinomiya, Shivashankar, Simon, Walter, Wu   +47 more
core   +1 more source

Mapping of origin of replication in Themococcales [PDF]

Bioinformation, 2010
Genome replication is a crucial and essential process for the continuity of life.In all organisms it starts at a specific region of the genome known as origin of replication (Ori) site. The number of Ori sites varies in prokaryotes and eukaryotes. Replication starts at a single Ori site in bacteria, but in eukaryotes multiple Ori sites are used for ...
Krishna K, Ojha, D, Swati
openaire   +2 more sources

Reciprocal control of viral infection and phosphoinositide dynamics

FEBS Letters, EarlyView.
Phosphoinositides, although scarce, regulate key cellular processes, including membrane dynamics and signaling. Viruses exploit these lipids to support their entry, replication, assembly, and egress. The central role of phosphoinositides in infection highlights phosphoinositide metabolism as a promising antiviral target.
Marie Déborah Bancilhon, Bruno Mesmin
wiley   +1 more source

On the Choice of the Right Plasmid Vector(s) in the Times of Synthetic Biology

Microbial Biotechnology
Plasmid vectors are to this day the fundamental tools in molecular biology, but their selection is often guided by convenience rather than informed choice. This article revisits the architectural and functional features that determine plasmid performance
Víctor deLorenzo, Esteban Martínez‐García   +1 more
doaj   +1 more source

Trapping DNA Replication Origins from the Human Genome [PDF]

, 2013
Synthesis of chromosomal DNA is initiated from multiple origins of replication in higher eukaryotes; however, little is known about these origins’ structures. We isolated the origin-derived nascent DNAs from a human repair-deficient cell line by blocking
Aladjem, Altschul, Berezney, Biamonti, Dazy, Dijkwel, Drouin, Eki, Fumio Hanaoka, Hansen, Kent, Okuno, Toshihiko Eki, Vassilev, Yasufumi Murakami   +14 more
core   +2 more sources

Mcm1 Binds Replication Origins [PDF]

Journal of Biological Chemistry, 2003
Mcm1 is an essential protein required for the efficient replication of minichromosomes and the transcriptional regulation of early cell cycle genes in Saccharomyces cerevisiae. In this study, we report that Mcm1 is an abundant protein that associates globally with chromatin in a punctate pattern.
Chang, Victoria K., Fitch, Michael J., Donato, Justin J., Christensen, Tim W., Merchant, Ankit Margaret, Tye, Bik Kwoon   +5 more
openaire   +3 more sources