Results 261 to 270 of about 1,052,584 (308)

SmallTalk: a novel small‐sized fusion tag for peptide expression and purification

FEBS Open Bio, EarlyView.
The SmallTalk fusion tag allows for the efficient expression and purification of soluble recombinant proteins or peptides in Escherichia coli. Testing with SmallTalk‐GFP confirmed that the proteins were soluble and folded correctly, while SmallTalk‐Bin1b maintained its antimicrobial activity against various bacterial isolates. This streamlined workflow
Atika Tariq, Nestor G. Casillas‐Vega, Alma Gomez‐Loredo, Xristo Zarate   +3 more
wiley   +1 more source

Naked cuticle is essential for Drosophila wing development beyond Wingless signaling

FEBS Open Bio, EarlyView.
Naked cuticle (Nkd), a Wnt signaling inhibitor, assumes extensive roles in Drosophila wing development. Overexpressing Nkd causes smaller, crumpled wings, while also perturbing multiple signaling pathways and developmental genes. A specific region (R1S) is critical for Nkd's function as a signaling integrator, offering new insights for studying its ...
Rui Wang, Ping Wang
wiley   +1 more source

The herpes simplex origin-binding protein: mechanisms for sequence-specific DNA binding and dimerization revealed by Cryo-EM. [PDF]

Nucleic Acids Res
Gustavsson E, Grünewald K, Elias P, Hällberg BM.   +3 more
europepmc   +1 more source

Autophagosome marker, LC3, is released extracellularly via several distinct pathways

FEBS Open Bio, EarlyView.
This study establishes a novel HiBiT‐tagging system for ultrasensitive detection of LC3, revealing multiple pathways for its extracellular secretion. It demonstrates that LC3 is released via both autophagy‐dependent and ‐independent mechanisms, including a novel route for nonlipidated LC3‐I.
Koki Saito, Masashi Arakawa, Koki Maeda, Eiji Morita   +3 more
wiley   +1 more source

License to Replicate: Mechanisms of Licensing Eukaryotic Origins for DNA Replication. [PDF]

Bioessays
Frisbie V, Bleichert F.
europepmc   +1 more source

Molecular impacts of Meier-Gorlin syndrome mutations on human origin licensing. [PDF]

J Biol Chem
Yang R, Hunker O, Kim J, Bleichert F.
europepmc   +1 more source

Application and research progress on artificial intelligence in the quality of Traditional Chinese Medicine. [PDF]

Front Pharmacol
Li MY, Zhu JQ, Liu XN, Wu MY, Dong K, Li XY, Gao P, Jiang ZH.   +7 more
europepmc   +1 more source

Mechanism of T7 Primase Selecting Active Priming Sites Among Genome. [PDF]

Biomolecules
Zhang Z, Chen J, Liu W, Wang Y, Cai H, Wang G.   +5 more
europepmc   +1 more source

The Wing Helix Domain Of Orc4 Is The Primary Determinant Of Dna Binding Specificity Of The Origin Recognition Complex

, 2015
Michael Dowicki
openalex   +1 more source

Percutaneous Arterial Closure for Inadvertent Subclavian Artery Cannulation During Central Venous Catheterization: A Case Report. [PDF]

Am J Case Rep
Zi G, Zhu GF.
europepmc   +1 more source