Targeting the Bacterial Orisome in the Search for New Antibiotics [PDF]
Frontiers in Microbiology, 2017 There is an urgent need for new antibiotics to combat drug resistant bacteria. Existing antibiotics act on only a small number of proteins and pathways in bacterial cells, and it seems logical that expansion of the target set could lead to development of
Julia E. Grimwade, Alan C. Leonard
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The Orisome: Structure and Function [PDF]
Frontiers in Microbiology, 2015 During the cell division cycle of all bacteria, DNA-protein complexes termed orisomes trigger the onset of chromosome duplication. Orisome assembly is both staged and stringently regulated to ensure that DNA synthesis begins at a precise time and only ...
Alan C Leonard, Julia E Grimwade
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Changing Perspectives on the Role of DnaA-ATP in Orisome Function and Timing Regulation [PDF]
Frontiers in Microbiology, 2019 Bacteria, like all cells, must precisely duplicate their genomes before they divide. Regulation of this critical process focuses on forming a pre-replicative nucleoprotein complex, termed the orisome.
Alan C. Leonard +3 more
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Blocking the Trigger: Inhibition of the Initiation of Bacterial Chromosome Replication as an Antimicrobial Strategy [PDF]
Antibiotics, 2019 All bacterial cells must duplicate their genomes prior to dividing into two identical daughter cells. Chromosome replication is triggered when a nucleoprotein complex, termed the orisome, assembles, unwinds the duplex DNA, and recruits the proteins ...
Alan Leonard, Julia E Grimwade
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Blocking, Bending, and Binding: Regulation of Initiation of Chromosome Replication During the Escherichia coli Cell Cycle by Transcriptional Modulators That Interact With Origin DNA [PDF]
Frontiers in Microbiology, 2021 Genome duplication is a critical event in the reproduction cycle of every cell. Because all daughter cells must inherit a complete genome, chromosome replication is tightly regulated, with multiple mechanisms focused on controlling when chromosome ...
Alan Leonard, Julia E Grimwade
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Low Affinity DnaA-ATP Recognition Sites in E. coli oriC Make Non-equivalent and Growth Rate-Dependent Contributions to the Regulated Timing of Chromosome Replication [PDF]
Frontiers in Microbiology, 2018 Although the mechanisms that precisely time initiation of chromosome replication in bacteria remain unclear, most clock models are based on accumulation of the active initiator protein, DnaA-ATP.
Prassanna Rao +4 more
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Link Between Antibiotic Persistence and Antibiotic Resistance in Bacterial Pathogens [PDF]
Frontiers in Cellular and Infection Microbiology, 2022 Both, antibiotic persistence and antibiotic resistance characterize phenotypes of survival in which a bacterial cell becomes insensitive to one (or even) more antibiotic(s).
Wolfgang Eisenreich +3 more
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The Role of the N-Terminal Domains of Bacterial Initiator DnaA in the Assembly and Regulation of the Bacterial Replication Initiation Complex [PDF]
Genes, 2017 The primary role of the bacterial protein DnaA is to initiate chromosomal replication. The DnaA protein binds to DNA at the origin of chromosomal replication (oriC) and assembles into a filament that unwinds double-stranded DNA.
Jolanta Zakrzewska-Czerwińska +1 more
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Unique and universal features of Epsilonproteobacterial origins of chromosome replication and DnaA-DnaA box interactions [PDF]
Frontiers in Microbiology, 2016 In bacteria, chromosome replication is initiated by the interaction of the initiator protein DnaA with a defined region of a chromosome at which DNA replication starts (oriC).
Pawel Jaworski +6 more
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Structure and Function of the Campylobacter jejuni Chromosome Replication Origin [PDF]
Frontiers in Microbiology, 2018 Campylobacter jejuni is the leading bacterial cause of foodborne infections worldwide. However, our understanding of its cell cycle is poor. We identified the probable C.
Pawel Jaworski +5 more
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