Building a bacterial orisome: emergence of new regulatory features for replication origin unwinding [PDF]
Molecular Microbiology, 2005 SummaryTriggering new rounds of chromosomal DNA replication during the bacterial cell cycle is exquisitely regulated, ensuring both proper timing and one round per cycle stringency. A critical first step is stable unwinding of oriC, the chromosomal replication origin, by multiprotein orisome complexes comprising the AAA+ initiator DnaA and modulator ...
Alan Leonard, Julia E Grimwade
exaly +6 more sources
Building the bacterial orisome: high-affinity DnaA recognition plays a role in setting the conformation of oriC DNA [PDF]
Molecular Microbiology, 2014 SummaryDuring assembly of the E. coli pre‐replicative complex (pre‐RC), initiator DnaA oligomers are nucleated from three widely separated high‐affinity DnaA recognition sites in oriC. Oligomer assembly is then guided by low‐affinity DnaA recognition sites, but is also regulated by a switch‐like conformational change in oriC mediated by sequential ...
Alan Leonard, Julia E Grimwade
exaly +5 more sources
The role of Helicobacter pylori DnaA domain I in orisome assembly on a bipartite origin of chromosome replication [PDF]
Molecular Microbiology, 2019 AbstractThe main roles of the DnaA protein are to bind the origin of chromosome replication (oriC), to unwind DNA and to provide a hub for the step‐wise assembly of a replisome. DnaA is composed of four domains, with each playing a distinct functional role in the orisome assembly.
Malgorzata Nowaczyk‐Cieszewska +5 more
openaire +3 more sources
Putative Cooperative ATP-DnaA Binding to Double-Stranded DnaA Box and Single-Stranded DnaA-Trio Motif upon Helicobacter pylori Replication Initiation Complex Assembly. [PDF]
Int J Mol Sci, 2021 oriC is a region of the bacterial chromosome at which the initiator protein DnaA interacts with specific sequences, leading to DNA unwinding and the initiation of chromosome replication.
Jaworski P +6 more
europepmc +2 more sources
AfsK-Mediated Site-Specific Phosphorylation Regulates DnaA Initiator Protein Activity in Streptomyces coelicolor. [PDF]
J Bacteriol, 2020 In all organisms, chromosome replication is regulated mainly at the initiation step. Most of the knowledge about the mechanisms that regulate replication initiation in bacteria has come from studies on rod-shaped bacteria, such as Escherichia coli and ...
Łebkowski T +4 more
europepmc +2 more sources
Initiation of DNA Replication. [PDF]
EcoSal Plus, 2010 Alan Leonard, Julia E Grimwade
exaly +2 more sources
Architecture of bacterial replication initiation complexes: orisomes from four unrelated bacteria [PDF]
Biochemical Journal, 2005 Bacterial chromosome replication is mediated by single initiator protein, DnaA, that interacts specifically with multiple DnaA boxes located within the origin (oriC). We compared the architecture of the DnaA–origin complexes of evolutionarily distantly related eubacteria: two Gram-negative organisms, Escherichia coli and Helicobacter pylori, and two ...
Anna, Zawilak-Pawlik +6 more
openaire +2 more sources
, 2022 oriC is a region of the bacterial chromosome at which the initiator protein DnaA interacts with specific sequences, leading to DNA unwinding and the initiation of chromosome replication.
Weigel, Christoph +6 more
core +1 more source
Identification Of Elements Essential For Replication Origin Activity In Escherichia coli [PDF]
, 2018 The increasing number of drug resistant pathogenic bacteria has created a growing health care crisis. Solving the crisis will require a number of solutions, among them being development of new antibiotics that target novel cell processes.
Rao, Prassanna
core +1 more source
Assembly of Helicobacter pylori initiation complex is determined by sequence-specific and topology-sensitive DnaA-oriC interactions [PDF]
, 2014 In bacteria, chromosome replication is initiated by binding of the DnaA initiator protein to DnaA boxes located in the origin of chromosomal replication (oriC). This leads to DNA helix opening within the DNA-unwinding element.
Mielke, T. +4 more
core +1 more source