Building a bacterial orisome: emergence of new regulatory features for replication origin unwinding [PDF]
Molecular Microbiology, 2005 SummaryTriggering new rounds of chromosomal DNA replication during the bacterial cell cycle is exquisitely regulated, ensuring both proper timing and one round per cycle stringency. A critical first step is stable unwinding of oriC, the chromosomal replication origin, by multiprotein orisome complexes comprising the AAA+ initiator DnaA and modulator ...
Alan C Leonard, Julia E Grimwade
exaly +6 more sources
The Role of the N-Terminal Domains of Bacterial Initiator DnaA in the Assembly and Regulation of the Bacterial Replication Initiation Complex [PDF]
Genes, 2017 The primary role of the bacterial protein DnaA is to initiate chromosomal replication. The DnaA protein binds to DNA at the origin of chromosomal replication (oriC) and assembles into a filament that unwinds double-stranded DNA.
Anna Zawilak-Pawlik +2 more
exaly +3 more sources
Building the bacterial orisome: high‐affinity DnaA recognition plays a role in setting the conformation of oriC DNA [PDF]
Molecular Microbiology, 2014 SummaryDuring assembly of the E. coli pre‐replicative complex (pre‐RC), initiator DnaA oligomers are nucleated from three widely separated high‐affinity DnaA recognition sites in oriC. Oligomer assembly is then guided by low‐affinity DnaA recognition sites, but is also regulated by a switch‐like conformational change in oriC mediated by sequential ...
Julia E Grimwade, Alan C Leonard
exaly +5 more sources
The role of Helicobacter pylori DnaA domain I in orisome assembly on a bipartite origin of chromosome replication [PDF]
Molecular Microbiology, 2019 AbstractThe main roles of the DnaA protein are to bind the origin of chromosome replication (oriC), to unwind DNA and to provide a hub for the step‐wise assembly of a replisome. DnaA is composed of four domains, with each playing a distinct functional role in the orisome assembly.
Malgorzata Nowaczyk‐Cieszewska +5 more
openaire +3 more sources
Putative Cooperative ATP-DnaA Binding to Double-Stranded DnaA Box and Single-Stranded DnaA-Trio Motif upon Helicobacter pylori Replication Initiation Complex Assembly. [PDF]
Int J Mol Sci, 2021 oriC is a region of the bacterial chromosome at which the initiator protein DnaA interacts with specific sequences, leading to DNA unwinding and the initiation of chromosome replication.
Jaworski P +6 more
europepmc +2 more sources
AfsK-Mediated Site-Specific Phosphorylation Regulates DnaA Initiator Protein Activity in Streptomyces coelicolor. [PDF]
J Bacteriol, 2020 In all organisms, chromosome replication is regulated mainly at the initiation step. Most of the knowledge about the mechanisms that regulate replication initiation in bacteria has come from studies on rod-shaped bacteria, such as Escherichia coli and ...
Łebkowski T +4 more
europepmc +2 more sources
Architecture of bacterial replication initiation complexes: orisomes from four unrelated bacteria [PDF]
Biochemical Journal, 2005 Bacterial chromosome replication is mediated by single initiator protein, DnaA, that interacts specifically with multiple DnaA boxes located within the origin (oriC). We compared the architecture of the DnaA–origin complexes of evolutionarily distantly related eubacteria: two Gram-negative organisms, Escherichia coli and Helicobacter pylori, and two ...
Anna, Zawilak-Pawlik +6 more
openaire +2 more sources
Initiation of DNA Replication [PDF]
EcoSal Plus, 2010 Alan C Leonard, Julia E Grimwade
exaly +2 more sources
, 2022 oriC is a region of the bacterial chromosome at which the initiator protein DnaA interacts with specific sequences, leading to DNA unwinding and the initiation of chromosome replication.
Weigel, Christoph +6 more
core +1 more source
Compaction and Segregation of DNA in Escherichia coli [PDF]
LifeConrad Louis Woldringh +1 more
exaly +1 more source