Results 71 to 80 of about 2,719,020 (292)

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

open access: yesFEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz   +6 more
wiley   +1 more source

Polarization‐resolved femtosecond Vis/IR spectroscopy tailored for resolving weak signals in biological samples using minimal sample volume

open access: yesFEBS Open Bio, EarlyView.
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

open access: yesFEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei   +3 more
wiley   +1 more source

Overview of etched calcite

open access: yes, 2008
Image Type: SEM Image, Scale Bar: NA, Magnification: 3000, Etch: No etching, Coating: Au/Pd, Instrument Location: UNM, kV: 15, Spot Sz ...
Melim, L.   +4 more
core  

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

open access: yesFEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee   +3 more
wiley   +1 more source

BONE MARROW TREPHINE BIOPSY: STILL A VITAL DIAGNOSTIC TOOL IN THE 21 CENTURY [PDF]

open access: yesKanem Journal of Medical Sciences, 2017
Trephine biopsy is an indispensable diagnostic tool in the 21 century, even though the technique has an ancient origin, its value in developing countries is still innovative.
Zarami AB, Bukar AA, Satumari NA
doaj  

Overview of etched crystals

open access: yes, 2005
Image Type: SEM Image, Scale Bar: 10um, Magnification: 1000, Etch: No etching, Coating: Au, Instrument Location: U.
Jones, Brian   +2 more
core  

Design as translation activity: a semiotic overview [PDF]

open access: yes, 2016
The paper moves from the following question: can the design activity, intended as inventive and project-making activity, be viewed also as a form of translation? The answer such question compels us to overcome a paradox, because design does not involve a
ZINGALE, SALVATORE, Salvatore Zingale
core   +1 more source

Refinement of amino‐acid conformation vs. difference density maps in time‐resolved serial femtosecond crystallography data analysis

open access: yesFEBS Open Bio, EarlyView.
The dFoCC pipeline starts with observed DED and resting‐state coordinates, which are then used to generate a library of triggered states. Correlation analysis of the calculated DED features of each candidate vs observed DED permits quantitative evaluation of candidate structural quality.
Meng Iao Fong   +3 more
wiley   +1 more source

Overview of entire sample

open access: yes, 2005
Image Type: SEM Image, Scale Bar: 1mm, Magnification: 14, Etch: No etching, Coating: Au, Instrument Location: U.
Jones, Brian   +2 more
core  

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