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Is the Oxygen Radical Absorbance Capacity (ORAC) Method a Peroxyl-radical Scavenging Assay?

open access: yesZeitschrift Fur Physikalische Chemie, 2010
The oxygen radical absorbance capacity (ORAC) method employs a water soluble azo-radical initiator, AAPH (2,2’-azobis(2-amidinopropane) dihydrochloride) as a free radical generator, by which the fluorescent probe fluorescein is damaged to result in the ...
Yoshimi Sueishi, Yashige Kotake
exaly   +3 more sources
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Oxygen Radical Absorbing Capacity of Anthocyanins

Journal of Agricultural and Food Chemistry, 1997
Anthocyanins are natural colorants belonging to the flavonoid family. They are widely distributed among flowers, fruits, and vegetables. Using the automated oxygen radical absorbance capacity (ORAC) assay developed in our laboratory, we determined the antioxidant capacity of 14 anthocyanins including the aglycons delphinidin, cyanindin, pelargonidin ...
Hong Wang, Guohua Cao, Ronald L. Prior
openaire   +1 more source

Variability of oxygen radical absorbance capacity (ORAC) in different animal species

Free Radical Research, 1998
The oxygen radical absorbance capacity (ORAC) was measured both in whole (ORAC-T) and deproteinized (ORAC-AS) plasma samples of human, pig, cow, rabbit, dog, cat, sheep, horse, dolphin, turkey, guinea-hen and chicken. In the 12 species, ORAC-T data, expressed as micromoles of peroxyl radicals trapped by 11 of sample, were found scattered between 8,600 ...
NINFALI, PAOLINO, ALUIGI G.
openaire   +3 more sources

Extending Applicability of the Oxygen Radical Absorbance Capacity (ORAC−Fluorescein) Assay

Journal of Agricultural and Food Chemistry, 2003
The ORAC-fluorescein (ORAC-FL) method recently validated using automatic liquid handling systems has now been adapted to manual handling and using a conventional fluorescence microplate reader. As calculated for Trolox, the precision of the method was
Dávalos Herrera, Alberto   +2 more
openaire   +3 more sources

Oxygen Radical Absorbing Capacity of Phenolics in Blueberries, Cranberries, Chokeberries, and Lingonberries

Journal of Agricultural and Food Chemistry, 2002
The antioxidant activity of phenolics in fruits of blueberry (Vaccinium corymbosum cv. Sierra), cranberry (Vaccinium macrocarpon cv. Ben Lear), wild chokeberry (Aronia melanocarpa), and lingonberry (Vaccinium vitis-idaea cv. Amberland) was determined in this study.
Wei, Zheng, Shiow Y, Wang
openaire   +2 more sources

Role of alkoxyl radicals on the fluorescein-based ORAC (Oxygen Radical Absorbance Capacity) assay

Free Radical Biology and Medicine, 2014
During the last decades the ORAC (Oxygen Radical Absorbance Capacity) assay has been widely employed to evaluate the in vitro antioxidant capacity of polyphenol-rich fruits, vegetables and beverages. The method employs fluorescein (FLH) as target molecule and AAPH (2,2'-azo-bis(2-amidinopropane)dihydrochloride) as the source of peroxyl radicals (ROO•).
E, Dorta   +5 more
openaire   +2 more sources

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