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Nutrition, 2014 The aim of this study was to evaluate the performance of the overall antioxidant of Lactobacillus fermentum LF31 bacterium with prebiotic supplement in human colon cultured cells.The antioxidant capability of L. fermentum LF31 has been assayed in vitro on human colon adenocarcinoma HT-29 cell line using the oxygen radical absorbance capacity method.The
Emanuele Persichetti +3 more
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, 2017 This chapter describes, from a critical point of view, the main in vitro methodologies employed to assess the antioxidant capacity (AC) of food samples. Considering the wide use of the oxygen radical absorbance capacity (ORAC) assay, it presents a chemical examination of this methodology. The methods for AC evaluation are based on different strategies;
Dorta Pérez, Eva +4 more
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Analytical Biochemistry, 2009
A new method is proposed for the evaluation of oxygen radical absorbance capacity (ORAC). The current fluorescence-based ORAC assay (ORAC-FL) is an indirect method that monitors the antioxidant's ability to protect the fluorescent probe from free radical-mediated damage, and an azo-radical initiator, AAPH (2,2-azobis(2-amidinopropane) dihydrochloride),
Hirotada Fujii +2 more
exaly +3 more sources
A new method is proposed for the evaluation of oxygen radical absorbance capacity (ORAC). The current fluorescence-based ORAC assay (ORAC-FL) is an indirect method that monitors the antioxidant's ability to protect the fluorescent probe from free radical-mediated damage, and an azo-radical initiator, AAPH (2,2-azobis(2-amidinopropane) dihydrochloride),
Hirotada Fujii +2 more
exaly +3 more sources
A Device for the Semiautomatic Determination of Oxygen-Radical Absorbance Capacity
Analytical Biochemistry, 2000The oxygen-radical absorbance capacity (ORAC) assay has become a standard method to quantify the antioxidative properties of phytonutrients in fruit and vegetable extracts. However, it is usually not possible to determine directly the contribution of specific phytonutrients to the total ORAC value.
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Journal of Agricultural and Food Chemistry, 2001
An improved method of oxygen radical absorbance capacity (ORAC) assay has been developed and validated using fluorescein (3',6'-dihydroxyspiro[isobenzofuran-1[3H],9'[9H]-xanthen]-3-one) as the fluorescent probe. Our results demonstrate that fluorescein (FL) is superior to B-phycoerythrin.
Boxin Ou, Ronald L Prior
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An improved method of oxygen radical absorbance capacity (ORAC) assay has been developed and validated using fluorescein (3',6'-dihydroxyspiro[isobenzofuran-1[3H],9'[9H]-xanthen]-3-one) as the fluorescent probe. Our results demonstrate that fluorescein (FL) is superior to B-phycoerythrin.
Boxin Ou, Ronald L Prior
exaly +3 more sources
Food Chemistry, 2012
Ovotransferrin-derived peptides showed synergistic effects with vitamin C, epigallocatechin gallate (EGCG), and caffeic acid, but not quercetin in our previous report. In this study, we further investigated the interactions between ovotransferrin-derived peptides and teas, based on the oxygen radical absorbance capacity (ORAC) assay. Our results showed
Wuyang Huang +2 more
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Ovotransferrin-derived peptides showed synergistic effects with vitamin C, epigallocatechin gallate (EGCG), and caffeic acid, but not quercetin in our previous report. In this study, we further investigated the interactions between ovotransferrin-derived peptides and teas, based on the oxygen radical absorbance capacity (ORAC) assay. Our results showed
Wuyang Huang +2 more
exaly +3 more sources
Journal of Physical Chemistry A, 2013
Various radical-scavenging activities (RSA) assessment assays are based on discrete mechanisms and on using different radical sources. Few studies have analyzed the structural significance of flavonoids in their peroxyl radical activities in the oxygen radical absorbance capacity (ORAC) assay.
Di Zhang, Yixiang Liu, Shengbao Cai
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Various radical-scavenging activities (RSA) assessment assays are based on discrete mechanisms and on using different radical sources. Few studies have analyzed the structural significance of flavonoids in their peroxyl radical activities in the oxygen radical absorbance capacity (ORAC) assay.
Di Zhang, Yixiang Liu, Shengbao Cai
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, 2009 Das erste Ziel der Diplomarbeit war die Entwicklung einer auf Mikrotiterplatten basierten Methode zur Erfassung der Oxygen-radical-absorbance-capacity (ORAC).Der zweite Ansatz umfasste die Bestimmung der antioxidativen Kapazität im Plasma von Ironmantriathleten innherhalb des FWF finanzierten Projektes "Risk- assessment of Ironman-triathlon ...
Stadlmayr, Barbara
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Oxygen Radical Absorbing Capacity of Anthocyanins
Journal of Agricultural and Food Chemistry, 1997Anthocyanins are natural colorants belonging to the flavonoid family. They are widely distributed among flowers, fruits, and vegetables. Using the automated oxygen radical absorbance capacity (ORAC) assay developed in our laboratory, we determined the antioxidant capacity of 14 anthocyanins including the aglycons delphinidin, cyanindin, pelargonidin ...
Hong Wang, Guohua Cao, Ronald L. Prior
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Variability of oxygen radical absorbance capacity (ORAC) in different animal species
Free Radical Research, 1998The oxygen radical absorbance capacity (ORAC) was measured both in whole (ORAC-T) and deproteinized (ORAC-AS) plasma samples of human, pig, cow, rabbit, dog, cat, sheep, horse, dolphin, turkey, guinea-hen and chicken. In the 12 species, ORAC-T data, expressed as micromoles of peroxyl radicals trapped by 11 of sample, were found scattered between 8,600 ...
NINFALI, PAOLINO, ALUIGI G.
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