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Is the Oxygen Radical Absorbance Capacity (ORAC) Method a Peroxyl-radical Scavenging Assay?

Zeitschrift für Physikalische Chemie, 2010
Abstract The oxygen radical absorbance capacity (ORAC) method employs a water soluble azo-radical initiator, AAPH (2,2’-azobis(2-amidinopropane) dihydrochloride) as a free radical generator, by which the fluorescent probe fluorescein is damaged to result in the loss of fluorescence.
Yoshimi Sueishi   +7 more
openaire   +1 more source

An oxygen radical absorbance capacity-like assay that directly quantifies the antioxidant’s scavenging capacity against AAPH-derived free radicals

Analytical Biochemistry, 2009
A new method is proposed for the evaluation of oxygen radical absorbance capacity (ORAC). The current fluorescence-based ORAC assay (ORAC-FL) is an indirect method that monitors the antioxidant's ability to protect the fluorescent probe from free radical-mediated damage, and an azo-radical initiator, AAPH (2,2-azobis(2-amidinopropane) dihydrochloride),
Shunji, Kohri   +7 more
openaire   +2 more sources

A Device for the Semiautomatic Determination of Oxygen-Radical Absorbance Capacity

Analytical Biochemistry, 2000
The oxygen-radical absorbance capacity (ORAC) assay has become a standard method to quantify the antioxidative properties of phytonutrients in fruit and vegetable extracts. However, it is usually not possible to determine directly the contribution of specific phytonutrients to the total ORAC value.
openaire   +2 more sources

Dietary oxygen radical absorbance capacity as a predictor of bone mineral density

Nutrition Research, 2002
Bone mineral density (BMD) was measured at the middle phalange of the non-dominant hand by dual x-ray absorptiometry in a random sample of 123 women aged 19 to 63 years. Monthly intake of foods with high Oxygen Radical Absorbance Capacity (ORAC) was obtained using a Food Frequency Questionnaire (FFQ), ORAC scores were calculated, and lifestyle factors ...
Danielle Lachnicht   +3 more
openaire   +1 more source

Antioxidant Capacity of Different Broccoli (Brassica oleracea) Genotypes Using the Oxygen Radical Absorbance Capacity (ORAC) Assay

Journal of Agricultural and Food Chemistry, 2002
Antioxidant capacity of hydrophilic and lipophilic extracts from eight broccoli genotypes was compared using the oxygen radical absorbance capacity (ORAC) assay. Each genotype was analyzed for carotenoid, tocopherol, ascorbic acid, and flavonoid content.
Anne C, Kurilich   +4 more
openaire   +2 more sources

Oxygen Radical Absorbance Capacity of Phycocyanin and Phycocyanobilin from the Food SupplementAphanizomenon flos-aquae

Journal of Medicinal Food, 2010
The oxygen radical absorbance capacity (ORAC) assay has been widely used to quantify peroxyl radical scavenging capacity of pure antioxidant compounds and antioxidant plant/food extracts. However, it has never been applied to natural compounds derived from microalgae-based dietary supplements, namely, phycocyanin (PC) and phycocyanobilin (PCB), for ...
BENEDETTI, SERENA   +3 more
openaire   +3 more sources

The olive oil oxygen radical absorbance capacity (DPPH assay) as a quality indicator

European Journal of Lipid Science and Technology, 2008
AbstractThe antioxidant properties of some single components and the total antioxidant activity of extra‐virgin olive oil have been evaluated by the oxygen radical absorbance capacity (ORAC) method. The total ORAC of the extra‐virgin olive oil was found to be positively correlated with the concentration of total polyphenols, which are important to the ...
ZULLO B. A, CIAFARDINI, Gino
openaire   +2 more sources

Improvement of the Lipophilic-Oxygen Radical Absorbance Capacity (L-ORAC) Method and Single-Laboratory Validation

Bioscience, Biotechnology, and Biochemistry, 2013
We improved the procedure for lipophilic-oxygen radical absorbance capacity (L-ORAC) measurement for better repeatability and intermediate precision. A sealing film was placed on the assay plate, and glass vials and microdispensers equipped with glass capillaries were used.
Jun, Watanabe   +6 more
openaire   +2 more sources

ORAC-Fluorescein Assay To Determine the Oxygen Radical Absorbance Capacity of Resveratrol Complexed in Cyclodextrins

Journal of Agricultural and Food Chemistry, 2008
The effect of the complexation of resveratrol with hydroxypropyl-beta-cyclodextrins (HP-beta-CDs) on the antioxidant capacity of the polyphenol is studied for the first time by means of the oxygen radical absorbance capacity (ORAC) method, using fluorescein (FL) as the fluorescent probe.
C, Lucas-Abellán   +5 more
openaire   +2 more sources

Antioxidant and Pro-Oxidant Effects of Green Tea Extracts in Oxygen Radical Absorbance Capacity Assay

Journal of Medicinal Food, 2007
Green tea extracts (GTEs) [water (GTE-W) and 75% ethanol (GTE-E)] were investigated to characterize their propensities to act as antioxidants or as pro-oxidants by analyzing oxygen radical absorbance capacity (ORAC) and scavenging capacity for hydroxyl radical.
Jung-Keun, Shin   +2 more
openaire   +2 more sources

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