Results 101 to 110 of about 47,430 (223)

Efficient production of 2′-fucosyllactose in Pichia pastoris through metabolic engineering and constructing an orthogonal energy supply system

Synthetic and Systems Biotechnology
2′-fucosyllactose (2′-FL) holds significant role in the infants' nutrition. While microbial production of 2′-FL has predominantly utilized Escherichia coli and Saccharomyces cerevisiae, the potential of Pichia pastoris, renowned for its robust NADPH ...
Yi Li, Xiang Wang, Kaidi Chen, Zhoukang Zhuang, Hongting Tang, Tao Yu, Wenbing Cao   +6 more
doaj   +1 more source

Large-scale functional expression of WT and truncated human adenosine A_2A receptor in Pichia pastoris bioreactor cultures

Microbial Cell Factories, 2008
Background The large-scale production of G-protein coupled receptors (GPCRs) for functional and structural studies remains a challenge. Recent successes have been made in the expression of a range of GPCRs using Pichia pastoris as an expression host.
Strange Philip G, Rana Rohini, Martinez Magdalena, Ruprecht Jonathan, Fiez-Vandal Cédric, Gras Adrien, Singh Shweta, Wagner Renaud, Byrne Bernadette   +8 more
doaj   +1 more source

Shear‐Wave Splitting Measured for Permanent Reservoir Monitoring Systems: An Example From the Snorre Field

Geophysical Prospecting, Volume 74, Issue 4, May 2026.
ABSTRACT Microseismic monitoring of offshore CO2$\rm CO_2$ storage projects is likely to include some deployment of offshore sensors. To improve the value proposition of this monitoring infrastructure, it is important to consider what other information can be gained about the CO2$\rm CO_2$ storage complex and the surrounding region.
Joseph Asplet, J‐Michael Kendall, Annie Jerkins, Tom Kettlety   +3 more
wiley   +1 more source

Comparing the expression of human DNA topoisomerase I in KM71H and X33 strains of Pichia pastoris

Electronic Journal of Biotechnology, 2016
Background: Human is an essential cellular enzyme that is found in all human cells. As this enzyme is upregulated in cancer cells exceedingly, it is used as a target for cancer chemotherapeutic drug development. As such, producing the in-house enzyme for
Ruo Ping Ang, Leong Sin Teoh, Mooi Kwai Chan, Noorizan Miswan, Boon Yin Khoo   +4 more
doaj   +1 more source

Structural and functional comparison of SARS-CoV-2-spike receptor binding domain produced in Pichia pastoris and mammalian cells

Scientific Reports, 2020
The yeast Pichia pastoris is a cost-effective and easily scalable system for recombinant protein production. In this work we compared the conformation of the receptor binding domain (RBD) from severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2 ...
Argentinian AntiCovid Consortium
doaj   +1 more source

Developments in the tools and methodologies of synthetic biology. [PDF]

, 2014
Synthetic biology is principally concerned with the rational design and engineering of biologically based parts, devices, or systems. However, biological systems are generally complex and unpredictable, and are therefore, intrinsically difficult to ...
Freemont, P, Kelwick, R, MacDonald, JT, Webb, AJ   +3 more
core   +1 more source

Utilization of Fermentation Process for the Production of Novel Plant‐Based Meat Analogs: A Review of Microorganisms, Processing, and Regulations

Comprehensive Reviews in Food Science and Food Safety, Volume 25, Issue 3, May 2026.
ABSTRACT The production of plant‐based meat analogues from various protein sources is an emerging application worldwide, driven by growing population and health concerns. Many microorganisms during fermentation play a crucial role in fermented plant‐based meat alternatives, together with structuring and analytical approaches in terms of nutritional ...
Fatma Beyza Özpınar, Sevda Dere, Serdar Altunay, Osman Sagdic, Enes Dertli   +4 more
wiley   +1 more source

Ekspresija rekombinantne ksiloza reduktaze na površini stanice kvasca P. pastoris

, 2018
Pichia pastoris je metilotrofni kvasac koji se upotrebljava kao uspješni sistem za ekspresiju heterolognih proteina. U ovom radu, P. pastoris je upotrijebljena za ekspresiju heterolognog rekombinantnog proteina ksiloza reduktaze na površini stanične stijenke kvasca.
openaire   +3 more sources

Tenebrio molitor‐Derived Enzyme Systems Enable Solvent‐Reduced Recovery of Intracellular Polyhydroxyalkanoates

Microbial Biotechnology, Volume 19, Issue 5, May 2026.
Multi‐enzyme systems from Tenebrio molitor enable solvent‐free recovery of microbial PHAs directly from wet biomass under mild aqueous conditions, achieving high polymer yields and integrity while reducing carbon footprint up to seven‐fold compared with conventional extraction methods.
Sanja Skaro Bogojevic, Brana Pantelic, Marijana Ponjavic, Tatjana Ilic‐Tomic, Dusan Milivojevic, Maciej Guzik, Marcin Rychwalski, Romanos Siaperas, Evangelos Topakas, Mónica Carvalheira, Filomena Freitas, Patrícia Concórdio‐Reis, Jasmina Nikodinovic‐Runic   +12 more
wiley   +1 more source

Vector for Pop-In/Pop-Out Gene Replacement in Pichia pastoris

BioTechniques, 2001
Gene replacement in yeast is often accomplished by using a counterselectable marker such as URA3. Although ura3 strains of Pichia pastoris have been generated, these strains are inconvenient to work with because they grow slowly, even in the presence of ...
Jon Soderholm, Brooke J. Bevis, Benjamin S. Glick   +2 more
doaj   +1 more source